Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SOJ16_RS09375 Genome accession   NZ_CP139957
Coordinates   1929540..1930595 (-) Length   351 a.a.
NCBI ID   WP_045175342.1    Uniprot ID   -
Organism   Caldicellulosiruptor danielii strain DSM 8977     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1924540..1935595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOJ16_RS09340 (SOJ16_001868) - 1925083..1925658 (-) 576 WP_045175335.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SOJ16_RS09345 (SOJ16_001869) - 1925655..1926209 (-) 555 WP_045175336.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SOJ16_RS09350 (SOJ16_001870) - 1926199..1926489 (-) 291 WP_045175337.1 late competence development ComFB family protein -
  SOJ16_RS09355 (SOJ16_001871) - 1926497..1927273 (-) 777 WP_045175338.1 prepilin peptidase -
  SOJ16_RS09360 (SOJ16_001872) - 1927327..1927767 (-) 441 WP_045175339.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SOJ16_RS09365 (SOJ16_001873) - 1927802..1928218 (-) 417 WP_045175340.1 type II secretion system protein -
  SOJ16_RS09370 (SOJ16_001874) - 1928258..1929469 (-) 1212 WP_045175341.1 type II secretion system F family protein -
  SOJ16_RS09375 (SOJ16_001875) pilT 1929540..1930595 (-) 1056 WP_045175342.1 type IV pilus twitching motility protein PilT Machinery gene
  SOJ16_RS09380 (SOJ16_001876) - 1930650..1932362 (-) 1713 WP_082054727.1 GspE/PulE family protein -
  SOJ16_RS09385 (SOJ16_001877) - 1932390..1935581 (-) 3192 WP_045175345.1 response regulator -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39650.76 Da        Isoelectric Point: 7.6394

>NTDB_id=911976 SOJ16_RS09375 WP_045175342.1 1929540..1930595(-) (pilT) [Caldicellulosiruptor danielii strain DSM 8977]
MDINSILKEAFLKEATDIHITPGVPPIYRIHGRLVRTDDSILTPEMVEEFVRQITNENQFKILEQKGEIDFSYSIKGVSR
FRVNVYKQRGSYSIAFRVIPVNIPPFETLGLPPVLKEFTKLNKGLVLVTGPTGSGKSTTLASLIDIINKERDVHIITLED
PIEYLHRHNKSIINQREIGSDTLSFANALRAALREDPDVILVGEMRDLETIAIALTAAETGHLVFSTLHTIGAAKTIDRI
IDVFPPHQQQQIRIQLSTVLQGVVSQQLLTRRDGKGRVVATEVMIVNPAIRNLIREAKTYQIQSIIQTHQRQGMITMEQS
LIDLYKRGLISREDAFNYATDFDFMQRLLSA

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=911976 SOJ16_RS09375 WP_045175342.1 1929540..1930595(-) (pilT) [Caldicellulosiruptor danielii strain DSM 8977]
ATGGATATTAATTCAATTCTCAAAGAAGCATTTCTCAAAGAGGCAACAGATATACATATTACACCAGGTGTTCCTCCAAT
TTATCGAATTCACGGTAGATTGGTACGGACAGACGATTCAATTTTGACGCCTGAAATGGTAGAGGAGTTTGTGCGACAGA
TTACTAATGAAAATCAGTTTAAAATTCTTGAGCAGAAAGGTGAGATAGACTTTTCTTACAGCATAAAAGGCGTAAGCAGA
TTCAGAGTAAATGTTTATAAACAAAGAGGTTCATATTCTATAGCTTTTAGAGTGATTCCAGTAAATATACCACCATTTGA
GACACTTGGCCTTCCACCAGTATTGAAGGAATTTACAAAATTGAACAAAGGACTTGTTTTAGTTACAGGTCCAACTGGTT
CAGGAAAATCCACAACTCTGGCATCATTGATTGATATAATCAATAAAGAAAGAGATGTACATATAATAACCTTAGAGGAT
CCAATAGAATATTTGCACAGGCATAACAAAAGTATTATTAACCAAAGAGAAATAGGGAGTGACACACTCAGTTTTGCAAA
TGCGCTGAGGGCAGCTTTGAGAGAAGATCCTGACGTAATCCTTGTCGGAGAGATGAGGGATTTAGAGACAATTGCGATAG
CTTTAACAGCTGCTGAAACAGGGCATCTTGTGTTTTCCACACTTCACACAATTGGAGCTGCGAAGACAATAGACCGTATA
ATTGACGTTTTTCCACCGCATCAGCAACAACAGATAAGAATTCAGCTATCAACAGTTTTACAGGGAGTTGTTTCTCAGCA
ACTTTTGACCCGTCGAGATGGTAAAGGTAGAGTTGTTGCGACAGAGGTAATGATAGTAAATCCAGCAATAAGAAACCTCA
TAAGGGAGGCTAAAACATATCAGATTCAGTCAATTATTCAGACCCATCAGCGACAGGGCATGATAACAATGGAACAGTCA
CTCATAGACTTGTACAAAAGAGGGCTTATCAGTCGTGAAGATGCATTCAATTATGCTACTGACTTTGATTTTATGCAAAG
ACTACTCAGTGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

51.445

98.575

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.149

99.145

0.507
  pilT Neisseria gonorrhoeae MS11

50.725

98.291

0.499
  pilT Acinetobacter baumannii D1279779

52.239

95.442

0.499
  pilT Acinetobacter nosocomialis M2

52.239

95.442

0.499
  pilT Acinetobacter baumannii strain A118

52.239

95.442

0.499
  pilT Legionella pneumophila strain Lp02

51.622

96.581

0.499
  pilT Legionella pneumophila strain ERS1305867

51.622

96.581

0.499
  pilT Vibrio cholerae strain A1552

51.796

95.157

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.796

95.157

0.493
  pilT Acinetobacter baylyi ADP1

51.964

94.302

0.49
  pilT Pseudomonas aeruginosa PAK

51.343

95.442

0.49
  pilT Pseudomonas stutzeri DSM 10701

51.045

95.442

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.076

100

0.413
  pilU Acinetobacter baylyi ADP1

40.882

96.866

0.396
  pilU Vibrio cholerae strain A1552

38.395

99.43

0.382