Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SJH90_RS04365 Genome accession   NZ_CP139937
Coordinates   877611..878591 (+) Length   326 a.a.
NCBI ID   WP_048254059.1    Uniprot ID   A0AAW8HTD0
Organism   Pluralibacter gergoviae strain L1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 872611..883591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SJH90_RS04330 ansB 872657..873703 (+) 1047 WP_048254055.1 L-asparaginase 2 -
  SJH90_RS04335 - 873886..874233 (+) 348 WP_045287464.1 hypothetical protein -
  SJH90_RS04340 hemW 874282..875421 (-) 1140 WP_048254056.1 radical SAM family heme chaperone HemW -
  SJH90_RS04345 - 875421..876008 (-) 588 WP_048254057.1 XTP/dITP diphosphatase -
  SJH90_RS04350 yggU 875996..876307 (-) 312 WP_043082074.1 DUF167 family protein YggU -
  SJH90_RS04355 - 876304..876870 (-) 567 WP_043082075.1 YggT family protein -
  SJH90_RS04360 - 876892..877596 (-) 705 WP_048254058.1 YggS family pyridoxal phosphate-dependent enzyme -
  SJH90_RS04365 pilT 877611..878591 (+) 981 WP_048254059.1 type IV pilus twitching motility protein PilT Machinery gene
  SJH90_RS04370 - 879015..879668 (+) 654 WP_043082078.1 LuxR C-terminal-related transcriptional regulator -
  SJH90_RS04375 ruvX 879705..880121 (-) 417 WP_048254060.1 Holliday junction resolvase RuvX -
  SJH90_RS04380 - 880121..880684 (-) 564 WP_045287471.1 YqgE/AlgH family protein -
  SJH90_RS04385 gshB 880783..881730 (-) 948 WP_043082081.1 glutathione synthase -
  SJH90_RS04390 rsmE 881753..882484 (-) 732 WP_043082082.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SJH90_RS04395 endA 882505..883242 (-) 738 WP_043082083.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35526.84 Da        Isoelectric Point: 7.2385

>NTDB_id=911875 SJH90_RS04365 WP_048254059.1 877611..878591(+) (pilT) [Pluralibacter gergoviae strain L1]
MDIEEIVALSVKHNVSDLHLCSAGQVRWRRRGSLERAPFPAPDLQLLLARWLGEAGVQEWLLQRQCDFSVETAGGRRLRA
SAFQHIRGCSLVLRLLPDACPQLSELWAPQALGELLHEESGLILVTGATGSGKSTTLAAMVEHLNRQRDGHILTLEDPVE
FIHRSARCLIQQREVGAHCASFADALRVALRQDPDVILIGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLLDA
FPAGEKEQVRGQLAGSLCAVLAQKLLPDKLGGRVALYELLVNTPAAANLIREGKSHQLPGIMQTGQQAGMLTFSQSFQQR
QAAGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=911875 SJH90_RS04365 WP_048254059.1 877611..878591(+) (pilT) [Pluralibacter gergoviae strain L1]
ATGGATATTGAAGAAATCGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCGGGCAGGTGCG
ATGGCGGCGGCGGGGGAGCCTCGAGCGGGCGCCATTCCCGGCGCCGGATCTGCAGCTCCTGCTGGCCCGCTGGCTCGGAG
AGGCGGGGGTACAGGAGTGGCTGCTGCAGCGGCAGTGCGACTTTTCCGTCGAAACGGCGGGCGGCAGGCGGCTGCGCGCC
AGCGCTTTCCAGCATATTCGCGGCTGCTCGTTGGTGCTGCGCCTGCTGCCCGACGCGTGCCCACAGCTCTCTGAACTGTG
GGCGCCGCAGGCCCTTGGCGAGCTGCTCCACGAGGAGAGCGGGCTTATCCTCGTCACCGGAGCGACCGGCAGCGGCAAGT
CCACCACGCTTGCCGCGATGGTGGAACACCTCAACCGGCAGCGGGACGGGCATATTCTGACCCTGGAAGACCCGGTAGAG
TTTATTCACCGCAGCGCCCGCTGCCTGATCCAGCAGCGGGAGGTCGGCGCCCACTGCGCCTCGTTTGCGGACGCGCTGCG
GGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGATTGGCGAACTGCGCGACAGCGAGACTATTCGCCTGGCGCTGACCG
CTGCGGAGACCGGGCACCTGGTTCTGGCGACGCTGCACACCCGGGGCGCGGCCCAGGCCGTTGAGCGGCTGCTGGACGCC
TTCCCCGCCGGCGAAAAAGAGCAGGTGCGCGGCCAGCTGGCGGGCAGCCTGTGCGCGGTGCTGGCCCAGAAGCTGCTGCC
AGATAAGCTGGGCGGGCGGGTGGCGCTGTACGAACTGCTGGTAAATACGCCCGCCGCCGCCAACCTGATCCGCGAGGGCA
AGAGCCATCAGCTGCCGGGCATCATGCAGACCGGGCAGCAGGCGGGCATGCTGACCTTCAGCCAGAGTTTTCAGCAGCGG
CAGGCCGCCGGGCGGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Neisseria meningitidis 8013

48.137

98.773

0.475
  pilT Neisseria gonorrhoeae MS11

47.826

98.773

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Legionella pneumophila strain Lp02

47.17

97.546

0.46
  pilT Legionella pneumophila strain ERS1305867

47.17

97.546

0.46
  pilT Acinetobacter nosocomialis M2

46.729

98.466

0.46
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.164

97.239

0.429
  pilU Vibrio cholerae strain A1552

41.925

98.773

0.414
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

38.318

98.466

0.377