Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SK095_RS02555 Genome accession   NZ_CP139195
Coordinates   556142..557290 (+) Length   382 a.a.
NCBI ID   WP_320547735.1    Uniprot ID   -
Organism   Pseudomonas sp. AN-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 551142..562290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SK095_RS02530 (SK095_02530) - 552029..552268 (+) 240 WP_136488340.1 helix-hairpin-helix domain-containing protein -
  SK095_RS02535 (SK095_02535) - 552378..553580 (-) 1203 WP_320546462.1 ISL3 family transposase -
  SK095_RS02540 (SK095_02540) - 554313..554510 (+) 198 WP_320547733.1 hypothetical protein -
  SK095_RS02545 (SK095_02545) - 554737..555096 (-) 360 WP_320547734.1 hypothetical protein -
  SK095_RS02550 (SK095_02550) - 555355..555858 (-) 504 WP_136488586.1 hypothetical protein -
  SK095_RS02555 (SK095_02555) pilU 556142..557290 (+) 1149 WP_320547735.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SK095_RS02560 (SK095_02560) - 557350..557628 (-) 279 WP_320547736.1 peptidylprolyl isomerase -
  SK095_RS02565 (SK095_02565) - 557761..559128 (-) 1368 WP_320547737.1 phosphate ABC transporter substrate-binding/OmpA family protein -
  SK095_RS02570 (SK095_02570) xthA 559229..560041 (-) 813 WP_320547738.1 exodeoxyribonuclease III -
  SK095_RS02575 (SK095_02575) tpx 560258..560755 (+) 498 WP_136488582.1 thiol peroxidase -
  SK095_RS02580 (SK095_02580) ppk2 560900..561721 (+) 822 WP_320547739.1 polyphosphate kinase 2 -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42570.53 Da        Isoelectric Point: 6.1969

>NTDB_id=907798 SK095_RS02555 WP_320547735.1 556142..557290(+) (pilU) [Pseudomonas sp. AN-1]
MDITAMLRILASQDGSDLYLSTGAPPCAKFNGVLRALNKDVMAPGDVGKIAHELMDAEQRADFERELEMNLAISLPNVGR
FRVNIFKQRNEVSIVARNIKLDIPRFEDLKLPPVLLDVILEKRGLVLFVGGTGSGKSTSLAALIDHRNRNHSGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFQAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVLLGTATIRDLVKRGEFSLIKEVMEKSRPLGMQTFDQAL
IDLVNEGAIDEEEAVKNADSANNVRLKLKLHRDGEGSQARPDDTSTWSLAPTADEEEEPQFF

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=907798 SK095_RS02555 WP_320547735.1 556142..557290(+) (pilU) [Pseudomonas sp. AN-1]
ATGGACATCACGGCAATGCTGCGCATCCTGGCCAGCCAGGATGGATCGGACCTCTACCTGTCGACCGGCGCGCCGCCCTG
CGCCAAGTTCAACGGAGTGCTGCGCGCCCTGAACAAGGACGTGATGGCGCCCGGAGATGTGGGGAAGATTGCCCACGAAC
TGATGGACGCCGAGCAGCGGGCGGACTTCGAGCGCGAGCTGGAGATGAACCTGGCGATCTCGCTGCCCAACGTCGGGCGC
TTCCGGGTCAACATCTTCAAGCAGCGCAACGAGGTGTCGATAGTCGCGCGCAACATCAAGCTCGACATCCCGCGCTTCGA
GGATCTCAAGCTGCCGCCGGTGTTGCTCGACGTGATCCTCGAGAAGCGCGGCCTGGTGCTGTTCGTCGGGGGTACCGGCT
CGGGCAAGTCGACTTCGCTGGCGGCGCTGATCGACCACCGCAACCGCAACCACAGCGGCCACATCATCACCATCGAGGAC
CCTGTGGAGTATGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCAGGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATACTGATCGGCGAGATCCGTGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGAGACCGGACACCTTGCGATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGTCGCCCGCAGCTGCTCAACGACCTCGGCAACAACCTCAAGGCGTTCGTTTCCCAGCG
CCTGGTCAAGACCGTCGACGGCAAGCGCCGCGCGGCGGTGGAGGTGCTGCTGGGCACCGCGACCATCCGCGATCTGGTCA
AGCGCGGCGAGTTCAGTCTGATCAAGGAGGTCATGGAGAAGTCGCGGCCGCTGGGCATGCAGACCTTCGACCAGGCGCTG
ATCGATCTGGTCAACGAGGGCGCGATCGACGAGGAGGAAGCTGTGAAGAACGCCGACTCGGCGAACAACGTGCGCCTCAA
GCTCAAGCTGCACCGCGACGGCGAGGGCAGCCAGGCCAGGCCCGACGATACCTCGACCTGGAGCCTGGCGCCGACGGCGG
ACGAGGAAGAAGAGCCGCAGTTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.022

93.455

0.524
  pilU Vibrio cholerae strain A1552

50.693

94.503

0.479
  pilU Acinetobacter baylyi ADP1

51.42

92.147

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.493

92.408

0.393
  pilT Pseudomonas stutzeri DSM 10701

41.983

89.791

0.377
  pilT Pseudomonas aeruginosa PAK

41.691

89.791

0.374
  pilT Legionella pneumophila strain Lp02

42.515

87.435

0.372
  pilT Legionella pneumophila strain ERS1305867

42.515

87.435

0.372
  pilT Neisseria meningitidis 8013

41.159

90.314

0.372
  pilT Neisseria gonorrhoeae MS11

41.159

90.314

0.372
  pilT Vibrio cholerae strain A1552

42.342

87.173

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.342

87.173

0.369
  pilT Acinetobacter baumannii strain A118

41.916

87.435

0.366
  pilT Acinetobacter nosocomialis M2

41.916

87.435

0.366
  pilT Acinetobacter baumannii D1279779

41.916

87.435

0.366
  pilT Acinetobacter baylyi ADP1

41.818

86.387

0.361