Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R0L47_RS14490 Genome accession   NZ_CP139173
Coordinates   3068622..3069638 (-) Length   338 a.a.
NCBI ID   WP_137741248.1    Uniprot ID   -
Organism   Pectobacterium polonicum strain LMG 31077     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3063622..3074638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R0L47_RS14475 (R0L47_14410) nsrR 3064017..3064442 (-) 426 WP_103160677.1 nitric oxide-sensing transcriptional repressor NsrR -
  R0L47_RS14480 (R0L47_14415) - 3064737..3066308 (+) 1572 WP_137741250.1 methyl-accepting chemotaxis protein -
  R0L47_RS14485 (R0L47_14420) - 3066821..3068653 (+) 1833 WP_137741249.1 diguanylate cyclase -
  R0L47_RS14490 (R0L47_14425) pilT 3068622..3069638 (-) 1017 WP_137741248.1 type IV pilus twitching motility protein PilT Machinery gene
  R0L47_RS14495 (R0L47_14430) - 3069660..3070373 (+) 714 WP_137741247.1 YggS family pyridoxal phosphate-dependent enzyme -
  R0L47_RS14500 (R0L47_14435) proC 3070460..3071281 (+) 822 WP_137741246.1 pyrroline-5-carboxylate reductase -
  R0L47_RS14505 (R0L47_14440) - 3071331..3071886 (+) 556 Protein_2843 YggT family protein -
  R0L47_RS14510 (R0L47_14445) yggU 3071883..3072173 (+) 291 WP_010295005.1 DUF167 family protein YggU -
  R0L47_RS14515 (R0L47_14450) - 3072205..3072798 (+) 594 WP_137741244.1 XTP/dITP diphosphatase -
  R0L47_RS14520 (R0L47_14455) hemW 3072791..3073933 (+) 1143 WP_137741243.1 radical SAM family heme chaperone HemW -
  R0L47_RS14525 (R0L47_14460) - 3074161..3074433 (+) 273 WP_137741242.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36938.28 Da        Isoelectric Point: 6.2762

>NTDB_id=907530 R0L47_RS14490 WP_137741248.1 3068622..3069638(-) (pilT) [Pectobacterium polonicum strain LMG 31077]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPCLTPEQVAQWCDVWLEPTQKEQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSVALRIIPFVQPSLDELHAPPVLATLLEKPNGLILITGATGSGKSTTLAAMVGTLNDSSDRHVITLE
DPIEFIHTSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGTAVGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=907530 R0L47_RS14490 WP_137741248.1 3068622..3069638(-) (pilT) [Pectobacterium polonicum strain LMG 31077]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGGCATCCGCCGGT
ATTACGTGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCATGTTTAACGCCGGAACAGGTGGCGCAATGGTGTGATG
TCTGGCTAGAGCCGACTCAGAAAGAACAATTGCGACAGGCTGGGCAGGTGGATGGCGCGTTGATGCTGCCTGATGGTCAG
CGCCTGCGGGTAAATGTGTTTCGTCAACGGGAAGGGTTATCTGTCGCGCTGCGTATTATCCCGTTTGTGCAACCTTCATT
GGATGAGCTACATGCGCCACCCGTCCTCGCGACACTGCTGGAGAAACCAAACGGGCTGATTCTGATTACCGGTGCGACGG
GCAGCGGTAAATCCACGACGCTGGCGGCAATGGTTGGAACGTTAAACGACAGCAGCGATCGCCATGTGATCACGCTGGAA
GATCCGATCGAGTTTATTCATACCAGCCGGCGCTGTCTGATCCAACAGCGAGAGATCGGTGCACACAGCGCGTCTTTTGC
TCAGGCGCTACGAGCGGCATTGCGGGAAGATCCCGATGTTATTCTACTGGGAGAACTGCGCGATACGGAGACGATTCGTC
TGGCGTTGACAGCGGCGGAAACCGGCCATTTGGTGCTATCAACGCTACATACACGCAGTGCATCGCAGGCCGTTGATCGG
CTAATTGATGTCTTCCCCGGTGAAGAGAAAGCGTATGTTCGCAGCCAGTTAGCGACCTGTTTGCAGGCTGTAGTGACGCA
AAAGCTGTTGCCCGCCGCGCAAGGTGGCCGAATCGCCCTTTATGAAGTGCTGACGGCGACAGCAGCAGTCAGCAACTTGA
TTCGTGAAGGGAAAACGCACCAGCTTCCCGGCTTGATTCAGACGGGAACCGCAGTGGGAATGCAAACGTTCGAACAAAGC
TATCAGCAGCGCTGTCGGGACGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.606

97.633

0.494
  pilT Vibrio cholerae strain A1552

50.606

97.633

0.494
  pilT Neisseria meningitidis 8013

50

98.225

0.491
  pilT Neisseria gonorrhoeae MS11

49.699

98.225

0.488
  pilT Legionella pneumophila strain Lp02

48.036

97.929

0.47
  pilT Legionella pneumophila strain ERS1305867

48.036

97.929

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.036

97.929

0.47
  pilT Pseudomonas aeruginosa PAK

48.036

97.929

0.47
  pilT Acinetobacter baylyi ADP1

47.734

97.929

0.467
  pilT Acinetobacter nosocomialis M2

47.13

97.929

0.462
  pilT Acinetobacter baumannii D1279779

47.13

97.929

0.462
  pilT Acinetobacter baumannii strain A118

47.13

97.929

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Pseudomonas stutzeri DSM 10701

40.24

98.521

0.396
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367