Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIM97_RS20095 Genome accession   NZ_CP139172
Coordinates   4488465..4489481 (-) Length   338 a.a.
NCBI ID   WP_129706501.1    Uniprot ID   -
Organism   Pectobacterium zantedeschiae strain CFBP 1357     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4483465..4494481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIM97_RS20080 (SIM97_20065) nsrR 4483845..4484270 (-) 426 WP_129706495.1 nitric oxide-sensing transcriptional repressor NsrR -
  SIM97_RS20085 (SIM97_20070) - 4484566..4486137 (+) 1572 WP_129706497.1 methyl-accepting chemotaxis protein -
  SIM97_RS20090 (SIM97_20075) - 4486664..4488496 (+) 1833 WP_129706499.1 diguanylate cyclase -
  SIM97_RS20095 (SIM97_20080) pilT 4488465..4489481 (-) 1017 WP_129706501.1 type IV pilus twitching motility protein PilT Machinery gene
  SIM97_RS20100 (SIM97_20085) - 4489503..4490216 (+) 714 WP_129706503.1 YggS family pyridoxal phosphate-dependent enzyme -
  SIM97_RS20105 (SIM97_20090) proC 4490314..4491135 (+) 822 WP_129707184.1 pyrroline-5-carboxylate reductase -
  SIM97_RS20110 (SIM97_20095) - 4491186..4491740 (+) 555 WP_010295003.1 YggT family protein -
  SIM97_RS20115 (SIM97_20100) yggU 4491737..4492027 (+) 291 WP_005974847.1 DUF167 family protein YggU -
  SIM97_RS20120 (SIM97_20105) rdgB 4492045..4492638 (+) 594 WP_129706505.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SIM97_RS20125 (SIM97_20110) hemW 4492631..4493773 (+) 1143 WP_129706507.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36914.14 Da        Isoelectric Point: 6.4954

>NTDB_id=907513 SIM97_RS20095 WP_129706501.1 4488465..4489481(-) (pilT) [Pectobacterium zantedeschiae strain CFBP 1357]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPCLTSEQVAQWCDAWLEPTQKEQLRLAGQVDGALMLPDGR
RLRINVFRQREGLSAALRIIPSVQPSLDALHAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMIGELNDSSDRHVITLE
DPIEFIHDSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKTYVRSQLATCLQAVVTQRLLPSAHGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGSATGMQTFEQS
YQQRCRDGFISHSCALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=907513 SIM97_RS20095 WP_129706501.1 4488465..4489481(-) (pilT) [Pectobacterium zantedeschiae strain CFBP 1357]
ATGGAATTGGATGAATGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGGCACCCACCGGT
GTTACGTGTGGATGGGAGGCTACAGCCTGAAAATACCTTACCATGTTTAACGTCGGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTAGAACCGACTCAGAAAGAGCAACTACGGCTGGCAGGGCAGGTGGACGGCGCGTTAATGTTGCCTGATGGACGG
CGCCTGCGGATAAATGTATTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGCATCATCCCATCTGTTCAACCTTCATT
GGATGCGCTACATGCGCCACCCGTTCTCTCGACACTTCTGGAAAAACCGAACGGACTGATCCTGATTACCGGTGCGACGG
GCAGCGGTAAATCCACGACGCTGGCGGCTATGATTGGGGAATTAAACGATAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATTCATGACAGTCGACGCTGCCTGATTCAACAACGAGAGATCGGTGCGCACAGTGCGTCTTTTGC
TCAGGCGCTACGGGCAGCGTTGAGGGAGGATCCTGATGTCATTCTGTTAGGAGAGCTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACAGCCGCGGAAACGGGTCATTTGGTGCTATCAACGTTACATACGCGCAGTGCATCACAGGCGGTCGACCGT
TTAATTGATGTCTTTCCAGGTGAAGAGAAAACCTATGTTCGCAGCCAGTTAGCAACCTGTTTGCAGGCGGTGGTGACGCA
AAGGTTGTTGCCTTCTGCACACGGCGGCCGCATCGCGCTTTATGAAGTGCTGACGGCGACAGCGGCAGTCAGCAACCTGA
TTCGTGAGGGAAAAACGCACCAGCTTCCCGGCCTTATTCAGACCGGATCTGCTACGGGAATGCAAACGTTCGAACAAAGC
TACCAGCAGCGCTGTCGGGATGGGTTTATTTCACACAGTTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

49.405

99.408

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.303

97.633

0.491
  pilT Vibrio cholerae strain A1552

50.303

97.633

0.491
  pilT Neisseria gonorrhoeae MS11

49.107

99.408

0.488
  pilT Legionella pneumophila strain Lp02

48.358

99.112

0.479
  pilT Legionella pneumophila strain ERS1305867

48.358

99.112

0.479
  pilT Acinetobacter baylyi ADP1

47.633

100

0.476
  pilT Pseudomonas stutzeri DSM 10701

47.761

99.112

0.473
  pilT Pseudomonas aeruginosa PAK

47.164

99.112

0.467
  pilT Acinetobacter nosocomialis M2

47.164

99.112

0.467
  pilT Acinetobacter baumannii D1279779

47.164

99.112

0.467
  pilT Acinetobacter baumannii strain A118

47.164

99.112

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.543

99.704

0.414
  pilU Pseudomonas stutzeri DSM 10701

39.763

99.704

0.396
  pilU Vibrio cholerae strain A1552

38.279

99.704

0.382
  pilU Acinetobacter baylyi ADP1

37.87

100

0.379