Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB83_RS07180 Genome accession   NZ_CP138970
Coordinates   1358329..1359405 (+) Length   358 a.a.
NCBI ID   WP_413747218.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9507     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1353329..1364405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB83_RS07155 rsgA 1353439..1354320 (-) 882 WP_413404603.1 ribosome small subunit-dependent GTPase A -
  SIB83_RS07160 - 1354340..1354582 (-) 243 WP_413404605.1 sulfurtransferase TusA family protein -
  SIB83_RS07165 dnaJ 1354585..1355715 (-) 1131 WP_413747216.1 molecular chaperone DnaJ -
  SIB83_RS07170 grpE 1355758..1356495 (-) 738 WP_413747318.1 nucleotide exchange factor GrpE -
  SIB83_RS07175 pilB/pilB1 1356591..1358318 (+) 1728 WP_413747217.1 GspE/PulE family protein Machinery gene
  SIB83_RS07180 pilT 1358329..1359405 (+) 1077 WP_413747218.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB83_RS07185 - 1359409..1360833 (+) 1425 WP_413747219.1 type II secretion system F family protein -
  SIB83_RS07190 - 1360847..1362136 (-) 1290 WP_413747220.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB83_RS07195 msrB 1362066..1362575 (-) 510 WP_413404618.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB83_RS07200 dusA 1362645..1363649 (+) 1005 WP_413747221.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB83_RS07205 - 1363674..1364252 (-) 579 WP_413747222.1 RNA recognition motif domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39323.01 Da        Isoelectric Point: 7.0976

>NTDB_id=906365 SIB83_RS07180 WP_413747218.1 1358329..1359405(+) (pilT) [Synechococcus sp. MIT S9507]
MELMIEDLMQELVKNGGSDLHIASGQPPYGRFSGKLRPMRDAALPEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIEMLNMPPVVVETSQRPRGLVLVTGPTGSGKTTTLAALLDHINHSRAEHILTI
EDPIEFVYKSDRSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPGQQTQIRVQLSGSLLAVFSQTLCQRNNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGELGM
QTLEKALANLVKNNTITKTEALSKASKEGELDRLISSP

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=906365 SIB83_RS07180 WP_413747218.1 1358329..1359405(+) (pilT) [Synechococcus sp. MIT S9507]
ATGGAGCTGATGATCGAGGACCTGATGCAGGAACTGGTGAAAAACGGAGGCAGTGACCTGCATATCGCCAGCGGCCAGCC
CCCCTACGGACGTTTCAGCGGCAAGCTGCGGCCGATGCGTGATGCAGCTCTGCCGGAGGAGAGCTGTAACCGGCTGATCT
TCTCGATGCTCAACAACAGCCAACGCAAGACGCTTGAACAGACATGGGAACTTGACTGCGCCTACGGACTCAAGGGCGTT
GCCCGCTTCCGGGTGAATGTGTATCGCCAGAAAGGAAGCTATGCGGCCTGCCTGCGAGCACTGGGAAGCACAATCCCAAG
CATCGAAATGCTGAATATGCCTCCGGTGGTGGTGGAAACCAGTCAACGACCGCGGGGGCTCGTTTTGGTGACTGGACCCA
CAGGCTCAGGCAAGACAACCACCCTGGCCGCATTGCTGGATCACATCAACCACAGCCGGGCCGAACACATTCTCACCATC
GAAGATCCGATCGAGTTTGTTTACAAAAGCGATCGCAGCCTGGTGCACCAGCGTCAGCTCAATGAGGACACTCGCAGCTT
TGGAAATGCACTCAGAGCAGCCCTGCGGGAAGACCCCGACGTGATCCTGGTCGGAGAGATGCGCGACCTGGAAACCATTC
AGCTGGCCATCACCGCCGCGGAGACCGGTCACCTCGTCTTCGGCACTCTGCACACCAGTTCCGCTGCCCAGACGGTGGAC
CGCATGGTGGATGTGTTTCCTCCCGGCCAGCAGACCCAGATCCGTGTGCAACTCTCCGGAAGCCTGTTGGCGGTGTTTTC
CCAGACGCTTTGCCAACGCAACAACCCCGCTCCAGGTCAGTTCGGCCGAGTGATGGCTCAGGAAATCATGATCAACACCC
CTGCCATTGCCAACCTGATCCGCGAGGGCAAGACCGCTCAGCTGTATTCACAGATTCAGACCGGCGGCGAGCTGGGGATG
CAGACCCTGGAAAAAGCACTGGCGAATCTGGTGAAAAACAACACAATCACCAAAACGGAAGCCCTATCCAAAGCCTCCAA
AGAAGGCGAACTGGATCGGCTGATCAGCTCTCCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.959

95.251

0.514
  pilT Pseudomonas stutzeri DSM 10701

53.644

95.81

0.514
  pilT Legionella pneumophila strain ERS1305867

53.709

94.134

0.506
  pilT Legionella pneumophila strain Lp02

53.709

94.134

0.506
  pilT Acinetobacter nosocomialis M2

51.603

95.81

0.494
  pilT Acinetobacter baumannii D1279779

51.603

95.81

0.494
  pilT Acinetobacter baumannii strain A118

51.603

95.81

0.494
  pilT Neisseria meningitidis 8013

50.729

95.81

0.486
  pilT Neisseria gonorrhoeae MS11

50.437

95.81

0.483
  pilT Acinetobacter baylyi ADP1

51.506

92.737

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.294

94.972

0.478
  pilT Vibrio cholerae strain A1552

50.294

94.972

0.478
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.857

97.765

0.478
  pilU Pseudomonas stutzeri DSM 10701

38.746

98.045

0.38
  pilU Vibrio cholerae strain A1552

38.235

94.972

0.363