Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB85_RS02590 Genome accession   NZ_CP138963
Coordinates   495750..496823 (+) Length   357 a.a.
NCBI ID   WP_413743373.1    Uniprot ID   -
Organism   Synechococcus sp. WH 6501     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 490750..501823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB85_RS02565 rsgA 490857..491738 (-) 882 WP_186991651.1 ribosome small subunit-dependent GTPase A -
  SIB85_RS02570 - 491758..492000 (-) 243 WP_067093421.1 sulfurtransferase TusA family protein -
  SIB85_RS02575 dnaJ 492003..493133 (-) 1131 WP_067093422.1 molecular chaperone DnaJ -
  SIB85_RS02580 grpE 493176..493913 (-) 738 WP_186991648.1 nucleotide exchange factor GrpE -
  SIB85_RS02585 pilB/pilB1 494009..495739 (+) 1731 WP_186989867.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  SIB85_RS02590 pilT 495750..496823 (+) 1074 WP_413743373.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB85_RS02595 - 496840..498117 (+) 1278 WP_186989863.1 type II secretion system F family protein -
  SIB85_RS02600 - 498092..499420 (-) 1329 WP_186989861.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB85_RS02605 msrB 499350..499859 (-) 510 WP_186989859.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB85_RS02610 dusA 499929..500933 (+) 1005 WP_186989857.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB85_RS02615 - 500959..501633 (-) 675 WP_186989855.1 RNA recognition motif domain-containing protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39174.73 Da        Isoelectric Point: 5.9350

>NTDB_id=906298 SIB85_RS02590 WP_413743373.1 495750..496823(+) (pilT) [Synechococcus sp. WH 6501]
MELMIEDLMQELVEKGGSDLHIASGQPPFGRFSGQLRAMREEALQEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIELLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHTRAEHILTI
EDPIEFVYKSDRSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRSNPEPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGEQGM
QTLEKALANLVSDGDIALQEALAKASKPAELERLVGS

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=906298 SIB85_RS02590 WP_413743373.1 495750..496823(+) (pilT) [Synechococcus sp. WH 6501]
ATGGAGCTGATGATCGAAGACCTCATGCAGGAACTGGTGGAGAAGGGAGGCAGTGACCTGCATATCGCCAGCGGCCAGCC
CCCCTTTGGACGCTTCAGCGGGCAACTGCGAGCAATGCGTGAGGAAGCTCTACAGGAAGAGAGCTGCAACCGGCTGATCT
TCTCGATGCTCAACAACAGCCAACGCAAGACTTTGGAGCAAACTTGGGAACTCGACTGCGCCTACGGACTCAAAGGAGTG
GCGCGTTTCCGGGTGAATGTTTATCGCCAGAAAGGCAGCTATGCGGCCTGCCTGAGAGCACTGGGCAGCACGATCCCAAG
CATCGAATTGCTGAACCTCCCACCCGTGGTGGTGGAAACCAGCAAGCGACCAAGAGGACTGGTGCTGGTCACCGGACCAA
CAGGCTCAGGCAAGACCACCACCCTGGCGGCGCTGCTCGATCACATCAATCACACCCGTGCCGAACACATCCTCACCATT
GAGGACCCAATCGAATTCGTCTACAAAAGCGACCGAAGCCTGGTGCACCAGCGCCAGCTCAATGAAGACACCCGCAGTTT
TGGCAATGCACTGAGAGCAGCCCTGCGCGAAGACCCGGACGTGATCCTGGTGGGTGAAATGCGCGACTTGGAAACCATTC
AGCTGGCCATCAGTGCCGCGGAGACCGGTCACCTCGTCTTCGGCACTCTGCACACCAGTTCTGCCGCCCAAACCGTGGAC
CGCATGGTGGATGTGTTCCCACCCGCACAGCAAACCCAGATCCGCGTGCAACTGTCCGGAAGTCTGGTGGCGGTCTTCTC
CCAGACGCTCTGTCGTCGCAGCAATCCCGAACCTGGTCAGTTTGGCCGAGTCATGGCGCAGGAAATCATGATCAATACGC
CGGCCATCGCCAACCTGATTCGCGAAGGCAAGACCGCCCAGCTGTACTCACAGATCCAAACCGGCGGTGAACAGGGGATG
CAGACCCTGGAAAAAGCCCTGGCAAATCTCGTGAGCGATGGCGACATCGCTCTACAGGAAGCATTGGCCAAAGCCAGCAA
ACCTGCTGAGCTTGAGCGACTTGTCGGCAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.198

96.359

0.513
  pilT Pseudomonas aeruginosa PAK

53.198

96.359

0.513
  pilT Acinetobacter nosocomialis M2

51.744

96.359

0.499
  pilT Acinetobacter baylyi ADP1

51.744

96.359

0.499
  pilT Acinetobacter baumannii D1279779

51.453

96.359

0.496
  pilT Acinetobacter baumannii strain A118

51.453

96.359

0.496
  pilT Legionella pneumophila strain ERS1305867

51.929

94.398

0.49
  pilT Legionella pneumophila strain Lp02

51.929

94.398

0.49
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

98.039

0.49
  pilT Neisseria meningitidis 8013

50.437

96.078

0.485
  pilT Neisseria gonorrhoeae MS11

50.146

96.078

0.482
  pilT Vibrio cholerae strain A1552

50

95.238

0.476
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

95.238

0.476
  pilU Vibrio cholerae strain A1552

40.299

93.838

0.378
  pilU Pseudomonas stutzeri DSM 10701

38.462

98.319

0.378