Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SIB85_RS02585 Genome accession   NZ_CP138963
Coordinates   494009..495739 (+) Length   576 a.a.
NCBI ID   WP_186989867.1    Uniprot ID   -
Organism   Synechococcus sp. WH 6501     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 489009..500739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB85_RS02555 murB 489584..490516 (+) 933 WP_186989871.1 UDP-N-acetylmuramate dehydrogenase -
  SIB85_RS02560 - 490541..490882 (+) 342 WP_186989870.1 YbaB/EbfC family nucleoid-associated protein -
  SIB85_RS02565 rsgA 490857..491738 (-) 882 WP_186991651.1 ribosome small subunit-dependent GTPase A -
  SIB85_RS02570 - 491758..492000 (-) 243 WP_067093421.1 sulfurtransferase TusA family protein -
  SIB85_RS02575 dnaJ 492003..493133 (-) 1131 WP_067093422.1 molecular chaperone DnaJ -
  SIB85_RS02580 grpE 493176..493913 (-) 738 WP_186991648.1 nucleotide exchange factor GrpE -
  SIB85_RS02585 pilB/pilB1 494009..495739 (+) 1731 WP_186989867.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  SIB85_RS02590 pilT 495750..496823 (+) 1074 WP_413743373.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB85_RS02595 - 496840..498117 (+) 1278 WP_186989863.1 type II secretion system F family protein -
  SIB85_RS02600 - 498092..499420 (-) 1329 WP_186989861.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB85_RS02605 msrB 499350..499859 (-) 510 WP_186989859.1 peptide-methionine (R)-S-oxide reductase MsrB -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62967.31 Da        Isoelectric Point: 4.7200

>NTDB_id=906297 SIB85_RS02585 WP_186989867.1 494009..495739(+) (pilB/pilB1) [Synechococcus sp. WH 6501]
MSDDRRHLELELLLQRTIPGELVSADEPSLLQELKAKGFDPVRHAALAERLTELLSKNVGIKDEVPAQEQAITETSQRSN
SAVATNSQTSAPEGSESESVAETATSYLKDFTVGGILEADPDEKAEDSTGPQDLESGLNDAERSPVIDLVDRILMQALEL
DASDIHVEPQQAGLQLRFRQDGVLQSQIETLPSRLVPAVTSRFKIMADLDIAERRVPQDGRIRRRFQNRTVDFRVNSLPS
RYGEKIVLRLLDSSATQLGLDKLITNAEALQLVRTLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIE
FALPGITQCQVNREKGFDFSQALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDAPSAIARLDEMGVE
PFMVSASLIGIVSQRLLRRVCPQCRIAYRPDPEELGRFGLMASNEADVSFFRANHHEGQADPCPMCQGSGYKGRVGVYEV
LRMNEALATTVAKGATTDMVRQLALEGGMKTLLGYSLDLVREGHTTLEEVGRMVLTDSGLESERRARALSTLTCSGCGGG
LQEGWLECPYCLTPRH

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=906297 SIB85_RS02585 WP_186989867.1 494009..495739(+) (pilB/pilB1) [Synechococcus sp. WH 6501]
GTGAGCGACGACCGCCGGCATCTGGAACTGGAACTTCTGTTGCAGCGAACCATCCCAGGCGAGCTCGTCAGCGCCGATGA
ACCCTCACTGCTCCAGGAACTGAAAGCGAAGGGGTTTGATCCTGTTCGTCACGCCGCGCTTGCCGAACGCCTGACGGAGC
TGCTCAGCAAGAACGTAGGGATTAAGGATGAAGTTCCAGCCCAAGAACAAGCGATCACAGAAACATCGCAGCGATCCAAC
TCCGCCGTCGCAACCAACAGCCAGACCTCGGCTCCGGAAGGTTCAGAAAGCGAAAGCGTGGCGGAAACAGCCACGTCTTA
CCTGAAGGATTTCACCGTCGGCGGGATTCTGGAAGCCGATCCAGACGAGAAAGCTGAGGACTCCACAGGACCGCAGGATC
TTGAATCAGGCCTCAACGATGCAGAACGCTCCCCCGTGATCGATCTGGTGGATCGCATCCTGATGCAGGCCCTTGAGCTG
GATGCAAGCGACATCCATGTGGAGCCTCAGCAAGCCGGACTTCAACTGCGCTTCCGTCAGGACGGAGTGCTGCAGAGTCA
GATCGAAACCCTTCCGAGCCGACTGGTGCCGGCCGTGACCTCCAGGTTCAAGATCATGGCGGACCTGGACATCGCCGAAC
GGCGCGTGCCCCAGGACGGTCGCATCCGGCGAAGGTTCCAGAACCGCACGGTGGATTTCCGCGTGAACAGCCTGCCGAGC
CGCTACGGGGAAAAGATCGTGCTGCGGCTGCTGGACAGTTCCGCCACTCAACTGGGACTCGACAAGCTGATTACCAATGC
TGAAGCACTTCAGCTCGTGCGCACCCTGGGATCCAAACCCTTCGGGATGATCCTGGTGACAGGGCCGACTGGATCGGGCA
AATCCACCACGCTGTATTCCCTGCTGGCCGAACGCAATGAACCGGGGATCAACATCTCCACAGTTGAGGATCCGATCGAA
TTCGCCCTACCAGGTATTACCCAATGCCAGGTGAACCGGGAAAAAGGCTTCGATTTCAGTCAGGCACTGAGAGCCTTCAT
GCGCCAGGACCCAGACGTGCTGCTGGTCGGCGAAACACGCGATCTGGAAACAGCGAAAACAGCCATCGAAGCTGCTCTCA
CTGGTCACCTGGTGCTGACCACGCTCCACTGCAATGACGCCCCTAGCGCCATCGCCAGACTCGACGAGATGGGTGTGGAG
CCTTTCATGGTCAGCGCCTCACTGATCGGGATCGTGTCCCAGCGTCTACTTCGGCGGGTCTGTCCCCAATGCCGTATTGC
CTACCGGCCAGATCCCGAAGAACTAGGTCGCTTCGGGCTGATGGCAAGCAACGAGGCGGACGTCAGTTTCTTCCGTGCCA
ACCACCATGAAGGCCAGGCCGATCCTTGCCCCATGTGTCAGGGCAGTGGTTACAAAGGGCGAGTCGGGGTCTACGAAGTG
CTGCGCATGAACGAAGCGCTGGCGACCACCGTGGCCAAGGGCGCCACCACCGACATGGTGCGCCAGCTGGCTCTTGAGGG
AGGCATGAAAACACTTCTGGGTTACAGCCTCGATCTAGTGAGAGAGGGGCACACAACCCTGGAAGAAGTGGGCCGCATGG
TGCTCACCGATTCCGGCCTGGAATCAGAGCGGCGGGCCAGAGCCCTGAGCACGCTCACCTGCAGTGGCTGTGGTGGCGGA
CTTCAGGAAGGTTGGCTGGAATGTCCTTACTGCCTGACCCCACGTCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(153-419)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

56.537

98.264

0.556