Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB74_RS11465 Genome accession   NZ_CP138959
Coordinates   2301261..2302337 (-) Length   358 a.a.
NCBI ID   WP_063396538.1    Uniprot ID   A0A162DI20
Organism   Prochlorococcus sp. MIT 1306     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2296261..2307337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB74_RS11440 - 2296548..2297150 (+) 603 WP_074159153.1 RNA recognition motif domain-containing protein -
  SIB74_RS11445 dusA 2297160..2298164 (-) 1005 WP_063396541.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB74_RS11450 msrB 2298234..2298740 (+) 507 WP_011824706.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB74_RS11455 - 2298667..2299989 (+) 1323 WP_063396540.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB74_RS11460 - 2299964..2301244 (-) 1281 WP_063396539.1 type II secretion system F family protein -
  SIB74_RS11465 pilT 2301261..2302337 (-) 1077 WP_063396538.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB74_RS11470 pilB/pilB1 2302348..2304264 (-) 1917 WP_063396537.1 GspE/PulE family protein Machinery gene
  SIB74_RS11475 grpE 2304263..2304976 (+) 714 WP_063396536.1 nucleotide exchange factor GrpE -
  SIB74_RS11480 dnaJ 2305021..2306157 (+) 1137 WP_011824711.1 molecular chaperone DnaJ -
  SIB74_RS11485 - 2306154..2306399 (+) 246 WP_011824712.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39343.82 Da        Isoelectric Point: 6.1032

>NTDB_id=906261 SIB74_RS11465 WP_063396538.1 2301261..2302337(-) (pilT) [Prochlorococcus sp. MIT 1306]
MDLMIEDLMEQLVQGGGSDLHIATGQPPYGRFSGELRPMTNTPLSEETCNKLIFSILNNSHRKTLEQIWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIESLNLPSVVIETSKRPRGLVLVTGPTGSGKSTTLASLLDHINHTRSEHILTI
EDPIEFIYKSDLSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPNQQTQIRVQLSGSLLAIFSQTLCRRLNPQPEQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGELGM
QTLEKALANLTKQGEISRSEAIAKASKPSELEQLLNDG

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=906261 SIB74_RS11465 WP_063396538.1 2301261..2302337(-) (pilT) [Prochlorococcus sp. MIT 1306]
ATGGATCTAATGATCGAGGACCTGATGGAACAGCTGGTGCAAGGTGGCGGCAGTGATCTGCACATTGCTACCGGCCAGCC
CCCGTATGGACGATTCAGCGGTGAATTAAGGCCAATGACCAATACCCCCCTATCCGAAGAAACATGCAACAAGCTGATTT
TCTCAATACTCAACAACAGTCATAGAAAGACTCTTGAACAAATCTGGGAGCTTGACTGTGCCTATGGGCTAAAGGGTGTG
GCGCGCTTTCGTGTGAATGTCTATCGACAGAAGGGAAGTTACGCCGCCTGTCTACGTGCTCTTGGCAGCACTATCCCGAG
TATTGAAAGTCTCAACCTGCCATCAGTAGTCATAGAAACCAGCAAACGACCAAGGGGATTAGTGCTAGTAACAGGACCAA
CCGGATCAGGCAAATCAACAACCCTTGCTTCCCTGCTGGACCACATCAATCACACCAGAAGCGAACATATCCTCACGATC
GAAGATCCAATCGAATTCATATACAAAAGCGATCTCAGCCTTGTGCACCAGCGACAGCTCAATGAAGACACACGCAGTTT
TGGCAATGCCCTGCGTGCGGCACTACGCGAAGATCCAGATGTGATCCTCGTGGGCGAAATGCGCGACCTCGAAACCATTC
AACTGGCCATCAGCGCCGCCGAAACCGGTCACCTCGTATTCGGCACCCTGCACACCAGCTCAGCGGCACAAACGGTCGAT
CGCATGGTTGATGTTTTCCCCCCAAATCAGCAAACCCAAATCAGAGTTCAACTCTCAGGAAGCCTGCTAGCCATTTTTTC
GCAAACCCTCTGCCGTCGCCTCAATCCACAACCAGAACAATTTGGGCGGGTCATGGCTCAAGAAATCTTGATCAACACCC
CAGCCACAGCAAACCTGATCAGAGAAGGCAAAACCGCACAGCTCTACTCCCAAATTCAAACCGGAGGAGAACTAGGGATG
CAAACACTAGAAAAAGCCCTTGCGAATCTGACTAAACAGGGTGAAATTAGCCGTTCAGAAGCCATCGCCAAAGCCAGTAA
ACCCAGCGAACTGGAGCAACTCCTCAACGATGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A162DI20

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.907

96.089

0.508
  pilT Pseudomonas stutzeri DSM 10701

52.326

96.089

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.292

98.603

0.486
  pilT Acinetobacter baylyi ADP1

50.291

96.089

0.483
  pilT Legionella pneumophila strain Lp02

51.039

94.134

0.48
  pilT Legionella pneumophila strain ERS1305867

51.039

94.134

0.48
  pilT Neisseria gonorrhoeae MS11

49.425

97.207

0.48
  pilT Acinetobacter baumannii strain A118

49.28

96.927

0.478
  pilT Acinetobacter nosocomialis M2

49.28

96.927

0.478
  pilT Acinetobacter baumannii D1279779

49.28

96.927

0.478
  pilT Neisseria meningitidis 8013

49.854

95.81

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.529

94.972

0.461
  pilT Vibrio cholerae strain A1552

48.529

94.972

0.461
  pilU Pseudomonas stutzeri DSM 10701

40.395

98.883

0.399
  pilU Acinetobacter baylyi ADP1

37.931

97.207

0.369