Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB66_RS03580 Genome accession   NZ_CP138958
Coordinates   700816..701892 (-) Length   358 a.a.
NCBI ID   WP_063406042.1    Uniprot ID   -
Organism   Prochlorococcus sp. MIT 1318     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 695816..706892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB66_RS03555 - 696133..696705 (+) 573 WP_413681386.1 RNA recognition motif domain-containing protein -
  SIB66_RS03560 dusA 696715..697719 (-) 1005 WP_063406045.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB66_RS03565 msrB 697789..698295 (+) 507 WP_063406054.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB66_RS03570 - 698222..699544 (+) 1323 WP_063406044.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB66_RS03575 - 699519..700799 (-) 1281 WP_063406043.1 type II secretion system F family protein -
  SIB66_RS03580 pilT 700816..701892 (-) 1077 WP_063406042.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB66_RS03585 - 701903..703945 (-) 2043 WP_236070174.1 GspE/PulE family protein -
  SIB66_RS03590 grpE 703944..704657 (+) 714 WP_063406040.1 nucleotide exchange factor GrpE -
  SIB66_RS03595 dnaJ 704702..705838 (+) 1137 WP_063406039.1 molecular chaperone DnaJ -
  SIB66_RS03600 - 705835..706080 (+) 246 WP_011129402.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39337.83 Da        Isoelectric Point: 6.1704

>NTDB_id=906246 SIB66_RS03580 WP_063406042.1 700816..701892(-) (pilT) [Prochlorococcus sp. MIT 1318]
MDLMIEDLMEQLVQGGGSDLHIATGQPPYGRFSGELKPMTNTPLSEETCNKLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIESLNLPPVVIETSKRPRGLVLVTGPTGSGKTTTLASLLDHINHTRSEHILTI
EDPIEFVYKSDLSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTNSAAQTVD
RMVDVFPPNQQTQIRVQLSGSLLAIFSQTLCRRQNPQPEQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGELGM
QTLEKALASLTKKGEISRSEAIAKASKPSELEQLLNDG

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=906246 SIB66_RS03580 WP_063406042.1 700816..701892(-) (pilT) [Prochlorococcus sp. MIT 1318]
ATGGATCTAATGATCGAGGACCTGATGGAACAGCTTGTTCAAGGTGGCGGCAGTGATCTGCACATTGCTACCGGCCAGCC
CCCGTATGGACGATTCAGCGGTGAATTAAAGCCAATGACCAATACCCCCCTCTCCGAAGAAACATGCAACAAGCTGATTT
TCTCAATGCTCAACAACAGTCAGAGAAAGACTCTTGAACAAACCTGGGAGCTTGACTGTGCCTATGGGCTAAAGGGTGTG
GCGCGCTTTCGTGTAAATGTCTATCGACAGAAGGGAAGTTACGCCGCCTGTCTACGGGCTCTTGGCAGCACGATCCCGAG
TATTGAAAGTCTCAACCTGCCACCAGTAGTCATAGAAACCAGCAAACGACCAAGGGGATTAGTGCTAGTAACAGGACCAA
CCGGATCAGGCAAAACAACAACCCTGGCTTCCCTGCTGGACCACATCAATCACACCAGAAGCGAACATATCCTCACGATC
GAAGATCCAATCGAATTCGTATACAAAAGCGATCTCAGCCTTGTGCACCAGCGACAGCTCAATGAAGACACACGCAGTTT
TGGCAATGCCCTGCGTGCGGCACTACGTGAAGATCCTGATGTGATCCTAGTGGGCGAAATGCGCGACCTGGAAACCATTC
AACTGGCCATCAGCGCCGCCGAAACAGGTCACCTCGTGTTCGGCACCCTGCACACCAACTCAGCGGCACAAACGGTCGAT
CGCATGGTCGATGTTTTCCCCCCAAACCAACAAACCCAAATCAGAGTTCAACTCTCAGGAAGCCTGCTAGCCATTTTTTC
GCAAACACTCTGCCGTCGTCAAAATCCACAACCAGAACAGTTTGGGCGAGTCATGGCTCAAGAAATCTTGATCAACACCC
CAGCCACAGCAAACCTGATCAGAGAAGGCAAAACTGCACAGCTCTACTCCCAAATTCAAACCGGAGGAGAACTAGGGATG
CAAACACTAGAAAAAGCCCTTGCGAGTCTGACTAAAAAGGGTGAAATTAGCCGCTCTGAAGCCATCGCCAAAGCCAGTAA
ACCCAGCGAACTGGAGCAACTCCTCAACGATGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.907

96.089

0.508
  pilT Pseudomonas stutzeri DSM 10701

52.326

96.089

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.858

98.603

0.492
  pilT Legionella pneumophila strain Lp02

51.632

94.134

0.486
  pilT Legionella pneumophila strain ERS1305867

51.632

94.134

0.486
  pilT Acinetobacter baylyi ADP1

50.291

96.089

0.483
  pilT Neisseria gonorrhoeae MS11

49.138

97.207

0.478
  pilT Neisseria meningitidis 8013

49.563

95.81

0.475
  pilT Acinetobacter nosocomialis M2

48.991

96.927

0.475
  pilT Acinetobacter baumannii D1279779

48.991

96.927

0.475
  pilT Acinetobacter baumannii strain A118

48.991

96.927

0.475
  pilT Vibrio cholerae strain A1552

47.941

94.972

0.455
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.941

94.972

0.455
  pilU Pseudomonas stutzeri DSM 10701

39.831

98.883

0.394
  pilU Acinetobacter baylyi ADP1

37.931

97.207

0.369
  pilU Vibrio cholerae strain A1552

38.053

94.693

0.36