Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB46_RS08395 Genome accession   NZ_CP138956
Coordinates   1582707..1583783 (+) Length   358 a.a.
NCBI ID   WP_413295703.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9452     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1577707..1588783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB46_RS08365 - 1577965..1578300 (+) 336 WP_011934443.1 YbaB/EbfC family nucleoid-associated protein -
  SIB46_RS08370 rsgA 1578302..1579168 (-) 867 WP_413295698.1 ribosome small subunit-dependent GTPase A -
  SIB46_RS08375 - 1579161..1579388 (-) 228 WP_413295699.1 sulfurtransferase TusA family protein -
  SIB46_RS08380 dnaJ 1579389..1580498 (-) 1110 WP_413295700.1 molecular chaperone DnaJ -
  SIB46_RS08385 grpE 1580498..1581184 (-) 687 WP_413295701.1 nucleotide exchange factor GrpE -
  SIB46_RS08390 pilB/pilB1 1581196..1582686 (+) 1491 WP_413295702.1 GspE/PulE family protein Machinery gene
  SIB46_RS08395 pilT 1582707..1583783 (+) 1077 WP_413295703.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB46_RS08400 - 1583783..1585012 (+) 1230 WP_413295704.1 type II secretion system F family protein -
  SIB46_RS08405 - 1585005..1586261 (-) 1257 WP_413295705.1 aminoacetone oxidase family FAD-binding enzyme -
  SIB46_RS08410 msrB 1586254..1586742 (-) 489 WP_043737539.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB46_RS08415 dusA 1586776..1587783 (+) 1008 WP_413295706.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB46_RS08420 - 1587787..1588452 (-) 666 WP_413295707.1 RNA recognition motif domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39283.86 Da        Isoelectric Point: 5.6934

>NTDB_id=906230 SIB46_RS08395 WP_413295703.1 1582707..1583783(+) (pilT) [Synechococcus sp. MIT S9452]
MELQIETLMEQLVEKGGSDLHLSAGLPPYGRFSGALRPMHEAPLAEESCNRLIFSLLNNNQRKQLEQNWELDCSYGLKGV
ARFRVNVYREKGCYAACLRALGSSIPSLEQLGLPDVVREISERPRGLVLVTGPTGSGKTTTLAALLNHINQQRAEHILTV
EDPIEFVYKSERSVIHQRELGEDTRSFANALKAALREDPDVILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQIRVQLSGSLVAVFAQTLCRRLNPPAGAFGRVMAQEIMVNTPALANLIREGKTAQIYSQLQTGGQYGM
QTLERALANLVDGGQVERGEALSKASKPDELMRLLGEA

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=906230 SIB46_RS08395 WP_413295703.1 1582707..1583783(+) (pilT) [Synechococcus sp. MIT S9452]
ATGGAGCTGCAGATCGAAACCCTGATGGAGCAGTTAGTGGAGAAAGGGGGCAGCGATCTGCACCTCTCCGCTGGATTACC
CCCCTACGGCCGCTTCAGTGGTGCCTTACGGCCCATGCATGAAGCGCCTCTGGCCGAGGAGAGCTGCAACCGCTTGATCT
TCAGCCTGCTCAACAACAACCAGCGCAAGCAGCTGGAGCAGAACTGGGAGCTGGATTGCTCCTACGGGCTCAAAGGCGTG
GCCCGTTTTCGGGTCAACGTCTACCGGGAAAAGGGGTGCTATGCCGCCTGCCTGCGGGCCCTGGGCAGCAGCATCCCCAG
CCTGGAGCAACTGGGCCTGCCCGATGTAGTGCGCGAGATCAGCGAGCGGCCGCGGGGTTTGGTACTGGTGACCGGACCCA
CGGGATCAGGCAAAACCACCACCTTGGCGGCACTGCTGAACCACATCAATCAACAGCGGGCCGAACACATCCTCACGGTG
GAAGATCCCATCGAGTTTGTGTACAAGTCAGAGCGCAGCGTGATTCACCAGCGTGAGCTCGGTGAAGACACCCGCAGCTT
TGCCAATGCCCTCAAGGCCGCCCTGCGGGAAGACCCCGACGTGATCCTGGTGGGGGAAATGCGTGATCTAGAAACCATCC
AACTCGCCATCACCGCCGCAGAAACGGGCCATTTGGTGTTTGGCACCCTGCACACCAGCTCAGCAGCGCAAACGGTGGAT
CGCATGGTGGACGTGTTTCCGCCCGAGCAACAAACCCAGATCCGCGTGCAACTCAGCGGCAGCCTGGTGGCCGTGTTTGC
TCAAACCCTCTGCCGGCGGCTCAACCCCCCTGCTGGGGCCTTTGGCCGGGTGATGGCCCAAGAAATCATGGTCAACACTC
CGGCGCTGGCCAACCTGATTCGCGAGGGCAAAACCGCCCAGATCTATTCGCAGCTGCAAACCGGCGGCCAATACGGCATG
CAAACCCTCGAGCGCGCCCTGGCCAACCTGGTGGACGGCGGCCAAGTGGAGCGGGGCGAAGCCCTGAGCAAAGCCAGCAA
ACCCGATGAACTGATGCGGTTGCTCGGTGAGGCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.149

97.486

0.508
  pilT Pseudomonas stutzeri DSM 10701

51.576

97.486

0.503
  pilT Vibrio cholerae strain A1552

52.187

95.81

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.187

95.81

0.5
  pilT Acinetobacter baylyi ADP1

50.716

97.486

0.494
  pilT Acinetobacter baumannii strain A118

50.571

97.765

0.494
  pilT Acinetobacter nosocomialis M2

50.571

97.765

0.494
  pilT Acinetobacter baumannii D1279779

50.571

97.765

0.494
  pilT Neisseria meningitidis 8013

52.395

93.296

0.489
  pilT Neisseria gonorrhoeae MS11

52.395

93.296

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.157

99.441

0.489
  pilT Legionella pneumophila strain Lp02

51.622

94.693

0.489
  pilT Legionella pneumophila strain ERS1305867

51.622

94.693

0.489
  pilU Pseudomonas stutzeri DSM 10701

38.395

97.486

0.374
  pilU Vibrio cholerae strain A1552

37.11

98.603

0.366