Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SIB46_RS08390 Genome accession   NZ_CP138956
Coordinates   1581196..1582686 (+) Length   496 a.a.
NCBI ID   WP_413295702.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9452     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1576196..1587686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB46_RS08360 murB 1577023..1577940 (+) 918 WP_413295697.1 UDP-N-acetylmuramate dehydrogenase -
  SIB46_RS08365 - 1577965..1578300 (+) 336 WP_011934443.1 YbaB/EbfC family nucleoid-associated protein -
  SIB46_RS08370 rsgA 1578302..1579168 (-) 867 WP_413295698.1 ribosome small subunit-dependent GTPase A -
  SIB46_RS08375 - 1579161..1579388 (-) 228 WP_413295699.1 sulfurtransferase TusA family protein -
  SIB46_RS08380 dnaJ 1579389..1580498 (-) 1110 WP_413295700.1 molecular chaperone DnaJ -
  SIB46_RS08385 grpE 1580498..1581184 (-) 687 WP_413295701.1 nucleotide exchange factor GrpE -
  SIB46_RS08390 pilB/pilB1 1581196..1582686 (+) 1491 WP_413295702.1 GspE/PulE family protein Machinery gene
  SIB46_RS08395 pilT 1582707..1583783 (+) 1077 WP_413295703.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB46_RS08400 - 1583783..1585012 (+) 1230 WP_413295704.1 type II secretion system F family protein -
  SIB46_RS08405 - 1585005..1586261 (-) 1257 WP_413295705.1 aminoacetone oxidase family FAD-binding enzyme -
  SIB46_RS08410 msrB 1586254..1586742 (-) 489 WP_043737539.1 peptide-methionine (R)-S-oxide reductase MsrB -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 54167.49 Da        Isoelectric Point: 5.2633

>NTDB_id=906229 SIB46_RS08390 WP_413295702.1 1581196..1582686(+) (pilB/pilB1) [Synechococcus sp. MIT S9452]
MQCCHVAGRPAPGSRSENPAILKAEKVRGAAVERFELDDSFESFAPPPPPDGPDLVESANDREASPVIQLADRILVQALS
RQASDIHIEPQEDGLHVRLRLDGVLQNAFETLPAKVIPALTSRFKIMADLDIAERRQPQDGRIRRQYQGNTVDFRVSVLP
SRFGEKIVLRLLDSSATELGLDLLITDSQALAEVRDMGSKPYGILLVTGPTGSGKSTTLYSLLAERNDPGLNISTVEDPI
EYTLAGITQTQVNREKGVDFAKALRAFMRQDPDVLLVGETRDLETAKTALEAALTGHLVLTTLHCNDAPSAVARLEEMGV
ETYLLSASLIGVLSQRLLRKLCTHCRQSYQPKEATLARFGLEASQNSAINFYQPPANNTCSHCHGLGYKGRIGVYEVMRV
NEEMAEAIGQGAGTDQLRHLALSNGMKTLLGYGLELARQGHTSLEEVERVLLTDSGLEQEIRARNLSTATCSTCGAGLRD
EWLDCPYCLTPRGTLQ

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=906229 SIB46_RS08390 WP_413295702.1 1581196..1582686(+) (pilB/pilB1) [Synechococcus sp. MIT S9452]
GTGCAGTGCTGCCATGTTGCTGGGAGGCCCGCCCCTGGATCAAGGTCGGAAAACCCAGCCATCCTGAAAGCAGAGAAAGT
CCGAGGAGCAGCGGTGGAACGCTTCGAACTCGACGACAGCTTTGAGAGCTTTGCGCCGCCGCCGCCGCCAGATGGGCCGG
ACCTGGTGGAGAGCGCCAACGATCGCGAGGCCTCGCCGGTGATCCAGCTGGCGGATCGCATCTTGGTGCAAGCCCTGAGC
CGCCAGGCCAGCGACATCCATATCGAACCGCAGGAGGACGGACTGCATGTGCGGTTGCGGCTTGATGGCGTGCTGCAGAA
CGCTTTTGAAACCCTGCCGGCCAAGGTGATTCCGGCACTCACCAGCCGCTTCAAAATCATGGCGGACCTGGACATCGCCG
AGCGCCGCCAACCCCAAGACGGCCGCATTCGCCGGCAGTATCAAGGCAACACCGTTGATTTCCGCGTCAGCGTGCTGCCG
AGCCGCTTCGGCGAAAAAATTGTGCTGCGGCTGCTGGATTCCAGTGCCACCGAACTGGGGCTGGATCTGCTGATCACCGA
CTCCCAGGCCTTGGCCGAGGTGCGAGACATGGGCAGCAAGCCCTACGGAATCCTGCTTGTCACAGGCCCAACCGGCTCAG
GAAAATCCACCACCCTCTACTCGCTGCTGGCTGAGCGCAACGATCCAGGGCTGAACATCTCCACGGTGGAAGACCCGATC
GAATACACCCTGGCTGGGATCACGCAAACCCAGGTGAACCGGGAGAAGGGGGTGGACTTCGCCAAGGCCCTGCGGGCCTT
CATGCGCCAAGACCCAGATGTGTTGCTGGTGGGGGAAACCCGTGATCTCGAAACGGCCAAAACCGCGCTGGAGGCGGCGC
TGACCGGCCACCTGGTGCTCACCACCTTGCACTGCAACGACGCGCCCAGTGCTGTAGCGCGGCTTGAGGAGATGGGGGTG
GAGACCTACCTGCTGAGCGCCTCACTGATCGGCGTGCTCTCCCAGCGGTTGCTGCGCAAGCTCTGCACCCACTGCCGCCA
GTCCTACCAACCCAAGGAGGCCACCTTGGCCCGCTTCGGTTTGGAGGCCAGCCAAAACAGCGCCATCAACTTCTACCAAC
CTCCGGCCAACAACACCTGCAGCCATTGCCATGGCCTTGGTTACAAGGGCCGCATCGGCGTGTATGAGGTGATGCGCGTC
AATGAGGAGATGGCAGAGGCCATCGGCCAAGGCGCCGGCACCGACCAGCTGCGCCACCTGGCCCTCAGCAATGGCATGAA
GACCCTGCTGGGCTACGGGTTGGAACTGGCCCGCCAAGGCCACACCAGCCTGGAGGAGGTGGAGCGGGTGCTGCTGACGG
ACTCCGGGCTCGAGCAGGAAATCAGGGCCCGCAACCTCAGCACTGCGACCTGTTCAACCTGCGGCGCTGGGCTGAGAGAT
GAATGGCTTGATTGCCCCTATTGCCTAACTCCTAGGGGCACACTGCAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-340)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

61.659

89.919

0.554
  pilB Vibrio campbellii strain DS40M4

47.772

81.452

0.389
  pilB Vibrio parahaemolyticus RIMD 2210633

47.407

81.653

0.387
  pilF Thermus thermophilus HB27

45.476

84.677

0.385
  pilB Vibrio cholerae strain A1552

47.512

81.048

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.891

77.823

0.365