Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OQH79_RS10705 Genome accession   NZ_AP025227
Coordinates   2400788..2401840 (-) Length   350 a.a.
NCBI ID   WP_124045616.1    Uniprot ID   -
Organism   Clostridium perfringens E strain CP118     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2395788..2406840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OQH79_RS10685 (CP118TE_21110) sigE 2396381..2397088 (-) 708 WP_003449468.1 RNA polymerase sporulation sigma factor SigE -
  OQH79_RS10690 (CP118TE_21120) spoIIGA 2397102..2397908 (-) 807 WP_124045614.1 sigma-E processing peptidase SpoIIGA -
  OQH79_RS10695 (CP118TE_21130) ftsZ 2398313..2399458 (-) 1146 WP_061415801.1 cell division protein FtsZ -
  OQH79_RS10700 (CP118TE_21140) - 2399472..2400710 (-) 1239 WP_124045615.1 cell division FtsA domain-containing protein -
  OQH79_RS10705 (CP118TE_21150) pilT 2400788..2401840 (-) 1053 WP_124045616.1 type IV pilus twitching motility protein PilT Machinery gene
  OQH79_RS10710 (CP118TE_21160) sigK 2402021..2402722 (-) 702 WP_061428820.1 RNA polymerase sporulation sigma factor SigK -
  OQH79_RS10715 (CP118TE_21170) - 2402785..2404452 (-) 1668 WP_124045617.1 penicillin-binding transpeptidase domain-containing protein -
  OQH79_RS10720 (CP118TE_21180) udk 2404570..2405196 (-) 627 WP_060794470.1 uridine kinase -
  OQH79_RS10725 (CP118TE_21190) - 2405196..2406425 (-) 1230 WP_003459619.1 U32 family peptidase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39020.68 Da        Isoelectric Point: 5.4480

>NTDB_id=90618 OQH79_RS10705 WP_124045616.1 2400788..2401840(-) (pilT) [Clostridium perfringens E strain CP118]
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTESYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQTIISQQLVETVDGDRTAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=90618 OQH79_RS10705 WP_124045616.1 2400788..2401840(-) (pilT) [Clostridium perfringens E strain CP118]
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTACGCCCTGAAGATACTGAATCATATGCTAGAG
AGATATTACAGGATGCATATGAAAAGTATGATGCTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGAGTTATATCTTTAGATATGCCAACTCTAGA
TAGTTTAGGATATCCTGAGACTTTAAAGGATATATGTAATCTTAAAAGGGGCCTTGTATTAGTTACAGGACCTACTGGAA
GTGGTAAAAGTACAACTTTAGCTGCTTTAATAAATGAAATAAATAGTAATAGGGAATCTCATATAATAACAATAGAAGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAGAGAGAGATAGGGAAGGACACTCTAAGTTATGAAAG
AGCCTTAAAAGCAGCACTTAGAGAGGATCCTGATGTTATTTTAATTGGAGAAATGAGAGATTTAGAGACTATTTCAACAG
CTATAACAGCAGCAGAGACTGGACACTTAGTGTTTTCAACTCTTCATACTATAGGAGCAGCTAAGACTATAGATAGAATA
GTAGATGTTTTTCCACCACATCAACAAGAGCAAATAAAAATACAGTTAGCTTCCGTTTTACAAACAATTATATCACAGCA
GTTAGTAGAAACAGTTGATGGAGATAGAACTGCGGCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAATTTAATAA
GAGAAGGAAAAACTCATCAAATAGAATCATCCATACAAACAGGAAGTAAATATGGAATGAGAACAATGGACATGGAACTT
GCTAATCTTTATAGAGAAGGTATAATAACACAGGAAACTGCAATGAACTCTGCTATAGATAGGGAGATTCTAAGTCGATT
ATTGATGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.711

98.857

0.491
  pilT Neisseria meningitidis 8013

49.709

98.286

0.489
  pilT Neisseria gonorrhoeae MS11

49.709

98.286

0.489
  pilT Legionella pneumophila strain Lp02

49.415

97.714

0.483
  pilT Legionella pneumophila strain ERS1305867

49.415

97.714

0.483
  pilT Pseudomonas aeruginosa PAK

50

96

0.48
  pilT Acinetobacter baylyi ADP1

50.602

94.857

0.48
  pilT Acinetobacter baumannii strain A118

49.702

96

0.477
  pilT Acinetobacter baumannii D1279779

49.702

96

0.477
  pilT Acinetobacter nosocomialis M2

49.702

96

0.477
  pilT Pseudomonas stutzeri DSM 10701

49.702

96

0.477
  pilT Vibrio cholerae strain A1552

50.765

93.429

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

93.429

0.474
  pilU Pseudomonas stutzeri DSM 10701

43.976

94.857

0.417
  pilU Vibrio cholerae strain A1552

40.356

96.286

0.389
  pilU Acinetobacter baylyi ADP1

39.823

96.857

0.386

Multiple sequence alignment