Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB69_RS12080 Genome accession   NZ_CP138949
Coordinates   2444541..2445617 (-) Length   358 a.a.
NCBI ID   WP_036913090.1    Uniprot ID   A0A0A2CN62
Organism   Prochlorococcus sp. MIT 0701     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2439541..2450617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB69_RS12055 - 2439822..2440430 (+) 609 WP_413314380.1 RNA recognition motif domain-containing protein -
  SIB69_RS12060 dusA 2440440..2441444 (-) 1005 WP_036913079.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB69_RS12065 msrB 2441514..2442020 (+) 507 WP_036913082.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB69_RS12070 - 2441947..2443269 (+) 1323 WP_036913084.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB69_RS12075 - 2443244..2444524 (-) 1281 WP_036913087.1 type II secretion system F family protein -
  SIB69_RS12080 pilT 2444541..2445617 (-) 1077 WP_036913090.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB69_RS12085 pilB/pilB1 2445628..2447544 (-) 1917 WP_036913091.1 GspE/PulE family protein Machinery gene
  SIB69_RS12090 grpE 2447543..2448256 (+) 714 WP_036913095.1 nucleotide exchange factor GrpE -
  SIB69_RS12095 dnaJ 2448301..2449437 (+) 1137 WP_036913098.1 molecular chaperone DnaJ -
  SIB69_RS12100 - 2449434..2449679 (+) 246 WP_036913100.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39439.97 Da        Isoelectric Point: 6.1032

>NTDB_id=906165 SIB69_RS12080 WP_036913090.1 2444541..2445617(-) (pilT) [Prochlorococcus sp. MIT 0701]
MDLMIEDLMEQLVQGGGSDLHIATGQPPYGRFSGELRPMTNTPLSEETCNKLIFSMLNNSQRKTLEQIWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIEILNLPPVVIETSKRPRGLVLVTGPTGSGKTTTLASLLDHINHTRSEHILTI
EDPIEFVYKSDLSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTNSAAQTVD
RMVDVFPPNQQTQIRVQLSGSLLAIFSQTLCRRQNPQPEHFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGELGM
QTLEKALANLTKQGEISRSEAIAKASKPSELEQLLNDG

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=906165 SIB69_RS12080 WP_036913090.1 2444541..2445617(-) (pilT) [Prochlorococcus sp. MIT 0701]
ATGGATCTAATGATCGAGGACCTGATGGAACAGCTGGTTCAAGGTGGCGGCAGTGATCTGCACATTGCTACCGGCCAGCC
CCCGTATGGACGATTCAGCGGCGAATTAAGGCCAATGACCAATACCCCCCTATCCGAAGAAACATGCAACAAGCTGATTT
TCTCAATGCTCAACAACAGTCAGAGAAAGACTCTTGAACAAATCTGGGAGCTTGACTGTGCCTATGGGCTAAAGGGTGTG
GCGCGCTTTCGTGTGAATGTCTATCGACAGAAGGGAAGTTACGCCGCCTGTCTACGTGCTCTGGGCAGCACTATCCCGAG
TATTGAAATTCTCAACCTGCCACCTGTAGTCATAGAAACCAGCAAACGACCAAGGGGATTAGTGCTAGTAACAGGACCAA
CCGGATCAGGCAAAACAACAACGCTTGCGTCCCTACTGGACCACATCAATCACACCAGAAGCGAACACATCCTCACGATC
GAAGATCCAATCGAATTCGTATACAAAAGCGATCTCAGTCTTGTGCACCAGCGACAGCTCAATGAAGACACACGCAGTTT
TGGCAATGCCCTGCGTGCGGCACTACGCGAGGATCCAGATGTGATCCTCGTGGGCGAAATGCGCGACCTGGAAACCATTC
AACTGGCCATCAGCGCCGCCGAAACAGGTCACCTCGTATTCGGCACCCTGCACACCAACTCGGCGGCACAAACGGTCGAT
CGCATGGTCGATGTTTTCCCCCCAAACCAGCAAACCCAAATCAGAGTTCAACTCTCAGGAAGCCTGCTAGCCATTTTTTC
GCAAACCCTCTGCCGGCGCCAAAATCCACAACCAGAACACTTTGGGCGGGTCATGGCTCAAGAAATCTTGATCAACACCC
CAGCCACAGCAAACCTGATCAGAGAAGGCAAAACCGCACAGCTCTACTCCCAAATTCAAACCGGAGGAGAACTAGGGATG
CAAACACTAGAAAAAGCCCTTGCGAATCTGACTAAACAGGGTGAAATTAGCCGCTCTGAAGCCATCGCCAAAGCCAGTAA
ACCCAGCGAACTGGAGCAACTCCTCAACGATGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A2CN62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.907

96.089

0.508
  pilT Pseudomonas stutzeri DSM 10701

52.326

96.089

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.858

98.603

0.492
  pilT Legionella pneumophila strain Lp02

51.632

94.134

0.486
  pilT Legionella pneumophila strain ERS1305867

51.632

94.134

0.486
  pilT Acinetobacter baylyi ADP1

49.568

96.927

0.48
  pilT Neisseria gonorrhoeae MS11

49.425

97.207

0.48
  pilT Acinetobacter nosocomialis M2

49.28

96.927

0.478
  pilT Acinetobacter baumannii strain A118

49.28

96.927

0.478
  pilT Acinetobacter baumannii D1279779

49.28

96.927

0.478
  pilT Neisseria meningitidis 8013

49.854

95.81

0.478
  pilT Vibrio cholerae strain A1552

48.235

94.972

0.458
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.235

94.972

0.458
  pilU Pseudomonas stutzeri DSM 10701

40.395

98.883

0.399
  pilU Acinetobacter baylyi ADP1

38.286

97.765

0.374
  pilU Vibrio cholerae strain A1552

37.941

94.972

0.36