Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SIB42_RS02790 Genome accession   NZ_CP138941
Coordinates   545001..546074 (+) Length   357 a.a.
NCBI ID   WP_413328354.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9508     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540001..551074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIB42_RS02765 rsgA 540108..540989 (-) 882 WP_067093780.1 ribosome small subunit-dependent GTPase A -
  SIB42_RS02770 - 541009..541251 (-) 243 WP_067093421.1 sulfurtransferase TusA family protein -
  SIB42_RS02775 dnaJ 541254..542384 (-) 1131 WP_067093422.1 molecular chaperone DnaJ -
  SIB42_RS02780 grpE 542427..543164 (-) 738 WP_067093423.1 nucleotide exchange factor GrpE -
  SIB42_RS02785 pilB/pilB1 543260..544990 (+) 1731 WP_067093424.1 GspE/PulE family protein Machinery gene
  SIB42_RS02790 pilT 545001..546074 (+) 1074 WP_413328354.1 type IV pilus twitching motility protein PilT Machinery gene
  SIB42_RS02795 - 546091..547368 (+) 1278 WP_067093426.1 type II secretion system F family protein -
  SIB42_RS02800 - 547343..548671 (-) 1329 WP_067093427.1 NAD(P)/FAD-dependent oxidoreductase -
  SIB42_RS02805 msrB 548601..549110 (-) 510 WP_067093428.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SIB42_RS02810 dusA 549180..550184 (+) 1005 WP_067093430.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SIB42_RS02815 - 550210..550839 (-) 630 WP_413328358.1 RNA recognition motif domain-containing protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39089.64 Da        Isoelectric Point: 5.4599

>NTDB_id=906078 SIB42_RS02790 WP_413328354.1 545001..546074(+) (pilT) [Synechococcus sp. MIT S9508]
MELMIEDLMQELVEKGGSDLHIASGQPPYGRFSGHLRAMRDEALQEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVCRQKGSYAACLRALGSTIPSIELLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDDINHTRAEHILTI
EDPIEFVYKSDRSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRSNPEPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGELGM
QTLEKALADLVSDGDIALQEALAKASKPAELERLVGS

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=906078 SIB42_RS02790 WP_413328354.1 545001..546074(+) (pilT) [Synechococcus sp. MIT S9508]
ATGGAGCTGATGATCGAGGACCTCATGCAGGAACTGGTGGAGAAGGGAGGCAGTGACCTGCATATCGCCAGCGGCCAGCC
CCCCTATGGACGCTTCAGCGGACACCTACGAGCGATGCGGGATGAGGCTCTGCAGGAGGAGAGTTGCAACCGTTTGATCT
TCTCGATGCTCAACAACAGCCAACGCAAGACTTTGGAGCAAACTTGGGAACTCGACTGCGCCTACGGACTCAAAGGAGTG
GCGCGTTTCCGGGTGAATGTTTGTCGCCAGAAAGGCAGCTATGCGGCCTGCCTTAGAGCACTGGGCAGCACGATCCCAAG
CATCGAATTGCTGAACCTCCCACCCGTGGTGGTGGAAACCAGCAAGCGACCACGAGGACTGGTGCTGGTCACCGGACCAA
CAGGCTCAGGCAAGACGACCACCCTGGCGGCGCTGCTCGATGACATCAATCACACCCGCGCCGAGCACATCCTCACGATC
GAAGACCCAATCGAATTCGTTTACAAAAGCGATCGAAGCCTGGTGCACCAGCGCCAGCTCAATGAAGACACCCGCAGTTT
TGGCAATGCACTCAGAGCAGCCCTGCGTGAAGACCCGGACGTGATCCTGGTGGGTGAAATGCGCGACCTGGAAACCATTC
AGCTGGCCATCAGTGCCGCGGAAACCGGTCACCTCGTCTTCGGCACTCTGCACACCAGTTCTGCCGCCCAAACCGTGGAC
CGCATGGTGGATGTGTTCCCGCCCGCACAGCAAACCCAGATCCGCGTGCAACTGTCCGGAAGTCTGGTGGCGGTCTTCTC
CCAGACGCTTTGTCGTCGCAGCAATCCCGAGCCTGGTCAGTTCGGCCGAGTAATGGCGCAGGAAATCATGATCAATACGC
CTGCCATCGCCAACCTGATTCGCGAAGGCAAGACCGCTCAGCTGTATTCACAGATTCAAACCGGAGGCGAACTGGGGATG
CAGACCCTGGAAAAAGCCCTGGCAGATCTTGTAAGTGATGGCGACATCGCTCTACAGGAAGCATTGGCGAAAGCCAGCAA
ACCTGCTGAGCTTGAACGGCTTGTCGGCAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.488

96.359

0.515
  pilT Pseudomonas stutzeri DSM 10701

53.488

96.359

0.515
  pilT Acinetobacter nosocomialis M2

51.744

96.359

0.499
  pilT Acinetobacter baylyi ADP1

51.744

96.359

0.499
  pilT Acinetobacter baumannii D1279779

51.453

96.359

0.496
  pilT Acinetobacter baumannii strain A118

51.453

96.359

0.496
  pilT Legionella pneumophila strain ERS1305867

51.929

94.398

0.49
  pilT Legionella pneumophila strain Lp02

51.929

94.398

0.49
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.714

98.039

0.487
  pilT Neisseria meningitidis 8013

50.437

96.078

0.485
  pilT Neisseria gonorrhoeae MS11

50.146

96.078

0.482
  pilT Vibrio cholerae strain A1552

50

95.238

0.476
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

95.238

0.476
  pilU Vibrio cholerae strain A1552

40.299

93.838

0.378
  pilU Pseudomonas stutzeri DSM 10701

38.462

98.319

0.378