Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RZE78_RS24100 Genome accession   NZ_CP138515
Coordinates   5119250..5120293 (+) Length   347 a.a.
NCBI ID   WP_015867574.1    Uniprot ID   -
Organism   Variovorax paradoxus strain 2u118     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5114250..5125293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZE78_RS24080 (RZE78_24080) - 5114476..5114937 (+) 462 WP_319427904.1 hypothetical protein -
  RZE78_RS24085 (RZE78_24085) - 5114959..5117931 (-) 2973 WP_319427905.1 L-glutamate gamma-semialdehyde dehydrogenase -
  RZE78_RS24090 (RZE78_24090) - 5118020..5118493 (+) 474 WP_319427906.1 Lrp/AsnC ligand binding domain-containing protein -
  RZE78_RS24095 (RZE78_24095) - 5118500..5119198 (-) 699 WP_319427907.1 YggS family pyridoxal phosphate-dependent enzyme -
  RZE78_RS24100 (RZE78_24100) pilT 5119250..5120293 (+) 1044 WP_015867574.1 type IV pilus twitching motility protein PilT Machinery gene
  RZE78_RS24105 (RZE78_24105) pilU 5120322..5121458 (+) 1137 WP_124958857.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RZE78_RS24110 (RZE78_24110) - 5121502..5122410 (+) 909 WP_319427908.1 NAD(P)-dependent oxidoreductase -
  RZE78_RS24115 (RZE78_24115) - 5122446..5123210 (-) 765 WP_319427909.1 BON domain-containing protein -
  RZE78_RS24120 (RZE78_24120) - 5123207..5123851 (-) 645 WP_319427910.1 SIS domain-containing protein -
  RZE78_RS24125 (RZE78_24125) - 5123935..5124306 (-) 372 WP_319427911.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38111.82 Da        Isoelectric Point: 6.8885

>NTDB_id=903290 RZE78_RS24100 WP_015867574.1 5119250..5120293(+) (pilT) [Variovorax paradoxus strain 2u118]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKSVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFAELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSAIQTGQGAGMQTLDQN
LTDLVRRNAISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=903290 RZE78_RS24100 WP_015867574.1 5119250..5120293(+) (pilT) [Variovorax paradoxus strain 2u118]
GTGGACATTACCCAGTTGCTGGCATTCAGCGTCAAGAACAAAGCCTCCGACCTGCATCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCCACGGCGACGTGCGGCGCATCAACGTCGATGCGCTCGACCACAAGTCGGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTATGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAATGCCTTCAACCAGGCGCGCGGCGCGGCCGCGGTGTTCCGGACCATTCCCTCGAAGATCCTCACGCTGGA
GCAGCTCAACGCGCCCAAGATTTTCGCGGAACTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACCGGGCCTACCGGCT
CGGGCAAGTCGACCACCCTGGCCGCGATGGTCAACTACCTGAACGAAAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTGTCGTTCTCCAA
CGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACGCCATCCTGGTGGGCGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CCATGACCGCGGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAAAAGGAAATGATCCGCGCCATGCTGTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGGGCGCGGGCATGCAGACGCTGGACCAGAAC
CTGACCGACCTCGTGCGCCGCAATGCCATTTCGGCGGCGGAAGCACGCGGCAAGGCCAAGATCCCCGAGAATTTCCCTGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395