Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R5H22_RS00590 Genome accession   NZ_CP137764
Coordinates   110810..111844 (+) Length   344 a.a.
NCBI ID   WP_012698997.1    Uniprot ID   M9YCN3
Organism   Azotobacter sp. NL3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 105810..116844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5H22_RS00555 (R5H22_00555) - 105944..106363 (-) 420 WP_012698990.1 DUF4426 domain-containing protein -
  R5H22_RS00560 (R5H22_00560) metW 106420..107013 (-) 594 WP_041807492.1 methionine biosynthesis protein MetW -
  R5H22_RS00565 (R5H22_00565) - 107021..108160 (-) 1140 WP_012698992.1 homoserine O-acetyltransferase -
  R5H22_RS00570 (R5H22_00570) - 108289..108588 (-) 300 WP_012698993.1 DUF167 domain-containing protein -
  R5H22_RS00575 (R5H22_00575) - 108588..109181 (-) 594 WP_012698994.1 YggT family protein -
  R5H22_RS00580 (R5H22_00580) proC 109196..110014 (-) 819 WP_012698995.1 pyrroline-5-carboxylate reductase -
  R5H22_RS00585 (R5H22_00585) - 110033..110737 (-) 705 WP_012698996.1 YggS family pyridoxal phosphate-dependent enzyme -
  R5H22_RS00590 (R5H22_00590) pilT 110810..111844 (+) 1035 WP_012698997.1 type IV pilus twitching motility protein PilT Machinery gene
  R5H22_RS00595 (R5H22_00595) - 112424..112846 (+) 423 WP_072432472.1 NINE protein -
  R5H22_RS00600 (R5H22_00600) - 112907..114178 (-) 1272 WP_012698999.1 dihydroorotase -
  R5H22_RS00605 (R5H22_00605) - 114178..115188 (-) 1011 WP_012699000.1 aspartate carbamoyltransferase catalytic subunit -
  R5H22_RS00610 (R5H22_00610) pyrR 115471..115974 (-) 504 WP_012699001.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  R5H22_RS00615 (R5H22_00615) ruvX 116018..116446 (-) 429 WP_012699002.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37918.62 Da        Isoelectric Point: 6.8403

>NTDB_id=900592 R5H22_RS00590 WP_012698997.1 110810..111844(+) (pilT) [Azotobacter sp. NL3]
MDITELLAFGARQGASDLHLSAGLPPMIRVDGDVRRIDLPPLDHKQVHALIHDIMNDKQRKDYETFLETDFSFEVPSVAR
FRVNAFNQNRGAGAVFRTIPSRVPSMDELEMGEVFRKIAEVPRGLVLVTGATGSGKSTTLAAMLDYLNGSRHQHILTIED
PIEFVHEPKKCLINQREVHRDTLGFAEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVISQTLLKRVGGGRVAAREIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMAL
KSLLSRGAISRESAREKAKIPDSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=900592 R5H22_RS00590 WP_012698997.1 110810..111844(+) (pilT) [Azotobacter sp. NL3]
ATGGACATCACCGAACTGCTCGCCTTCGGCGCCAGGCAGGGCGCGTCGGACCTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCGACCTGCCGCCGCTGGACCACAAGCAGGTGCACGCACTGATCCACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAAACGTTTCTCGAAACCGACTTCTCCTTCGAGGTGCCGAGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTGTTCCGCACCATTCCGTCGCGCGTCCCGAGCATGGA
CGAGCTGGAGATGGGCGAGGTGTTCAGGAAGATCGCCGAGGTGCCGCGCGGCCTGGTGCTGGTCACCGGCGCCACTGGCT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTACCTGAATGGCAGCAGGCACCAGCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAGCCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCTGGGCTTCGCCGA
GGCGCTGCGTTCGGCGCTGCGCGAGGACCCGGACGTCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTTCCCGCGGAGGAGAAGGCGATGGTCCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAGGGTCGGCGGCGGCCGGGTGGCGGCCCGCGAGATCATGATCGGCACCCCGGCGATCCGCAATCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTGGACATGGCGTTG
AAGAGCCTGCTGTCCAGGGGCGCGATCAGCCGCGAGAGCGCCCGCGAGAAGGCGAAGATTCCCGACAGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M9YCN3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

80.233

100

0.802
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

71.302

98.256

0.701
  pilT Legionella pneumophila strain ERS1305867

71.302

98.256

0.701
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.941

98.837

0.672
  pilT Vibrio cholerae strain A1552

67.941

98.837

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Vibrio cholerae strain A1552

41.888

98.547

0.413
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398