Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LDO91_RS16765 Genome accession   NZ_AP025149
Coordinates   3730704..3731741 (-) Length   345 a.a.
NCBI ID   WP_101112564.1    Uniprot ID   A0AAV5NWA1
Organism   Vibrio penaeicida strain IFO 15642     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3725704..3736741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO91_RS16735 - 3725921..3726622 (+) 702 WP_126608178.1 endonuclease -
  LDO91_RS16740 rsmE 3726721..3727452 (+) 732 WP_126608179.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LDO91_RS16745 gshB 3727467..3728417 (+) 951 WP_101112561.1 glutathione synthase -
  LDO91_RS16750 - 3728453..3729016 (+) 564 WP_101112562.1 YqgE/AlgH family protein -
  LDO91_RS16755 ruvX 3729063..3729491 (+) 429 WP_101112582.1 Holliday junction resolvase RuvX -
  LDO91_RS16760 pilU 3729589..3730695 (-) 1107 WP_126608180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LDO91_RS16765 pilT 3730704..3731741 (-) 1038 WP_101112564.1 type IV pilus twitching motility protein PilT Machinery gene
  LDO91_RS16770 - 3731765..3732475 (+) 711 WP_126608181.1 YggS family pyridoxal phosphate-dependent enzyme -
  LDO91_RS16775 proC 3732538..3733356 (+) 819 WP_126608182.1 pyrroline-5-carboxylate reductase -
  LDO91_RS16780 - 3733385..3733942 (+) 558 WP_126608183.1 YggT family protein -
  LDO91_RS16785 yggU 3733942..3734232 (+) 291 WP_126608184.1 DUF167 family protein YggU -
  LDO91_RS16790 - 3734256..3734687 (+) 432 WP_126608185.1 DUF4426 domain-containing protein -
  LDO91_RS16795 - 3734778..3735377 (+) 600 WP_101112570.1 XTP/dITP diphosphatase -
  LDO91_RS16800 hemW 3735384..3736559 (+) 1176 WP_126608186.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37994.16 Da        Isoelectric Point: 6.0472

>NTDB_id=90052 LDO91_RS16765 WP_101112564.1 3730704..3731741(-) (pilT) [Vibrio penaeicida strain IFO 15642]
MDIAELLDFSVKHNASDLHLSAGVPPMVRVDGDVRKLGVPAFTHADVHRLVFEIMNDAQRAEFEEKLETDFSFELPNVGR
FRVNAFNQSRGSSAVFRTIPTEVPSLEQLDAPDIFTQISNYEKGLVLVTGPTGSGKSTTLAAMVDNINRNHNKHILTIED
PIEFVHQSNKCLINQREVHRDTHSFNAALRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGTDKDMVRSMLSESLRAVISQKLLKRLGGGRAACHEIMLATPAIRNLIREDKVAQMYSVIQTGGAMGMQTTEQHA
KQLIAQGVVDADEVRAKLESDSFGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=90052 LDO91_RS16765 WP_101112564.1 3730704..3731741(-) (pilT) [Vibrio penaeicida strain IFO 15642]
ATGGATATCGCTGAGTTACTGGATTTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCTGCTGGAGTTCCTCCAAT
GGTGCGTGTAGATGGCGATGTACGTAAGTTAGGTGTCCCTGCTTTTACACACGCCGATGTTCATCGACTGGTCTTTGAAA
TCATGAACGATGCGCAACGAGCAGAGTTTGAAGAAAAGCTGGAAACCGACTTTTCCTTCGAGCTGCCAAATGTCGGGCGT
TTTCGTGTCAATGCGTTTAACCAATCCCGTGGCAGTTCTGCGGTGTTCCGTACGATTCCTACTGAAGTACCTAGCTTGGA
GCAACTTGATGCCCCAGATATCTTTACTCAAATTTCCAATTATGAGAAAGGGCTTGTTCTGGTAACAGGACCAACAGGTT
CAGGTAAGTCAACCACACTGGCAGCCATGGTCGACAATATCAATCGCAACCATAACAAACACATTTTGACGATTGAAGAC
CCGATCGAATTCGTACACCAGAGCAATAAATGTTTGATCAACCAGCGTGAAGTACACCGCGATACACACAGCTTTAATGC
GGCACTTCGAAGCGCACTGCGTGAAGATCCGGATGTGATTCTTGTGGGCGAGCTCCGTGATAAAGAAACCATTAGCTTGG
CATTGACGGCGGCGGAAACCGGACACCTCGTATTTGGCACCTTGCACACCAGTAGCGCGGCGAAAACCGTCGACCGAATC
ATTGATGTGTTCCCGGGTACCGATAAAGACATGGTGCGTTCTATGCTTTCTGAATCATTACGTGCGGTGATTTCGCAAAA
GTTGTTGAAGCGTTTGGGTGGTGGCCGAGCTGCGTGTCACGAAATCATGTTAGCGACACCTGCGATTCGCAACCTTATCC
GTGAAGACAAAGTCGCTCAAATGTATTCCGTGATCCAAACGGGCGGGGCGATGGGAATGCAAACGACAGAGCAACACGCA
AAGCAGCTGATTGCACAAGGTGTGGTAGATGCAGATGAAGTCCGCGCCAAGCTTGAATCAGACTCCTTCGGCTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.377

100

0.864
  pilT Vibrio cholerae strain A1552

86.377

100

0.864
  pilT Acinetobacter baumannii D1279779

71.802

99.71

0.716
  pilT Acinetobacter baumannii strain A118

71.802

99.71

0.716
  pilT Acinetobacter nosocomialis M2

71.512

99.71

0.713
  pilT Acinetobacter baylyi ADP1

70.058

99.71

0.699
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Pseudomonas aeruginosa PAK

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

69.301

95.362

0.661
  pilT Legionella pneumophila strain ERS1305867

69.301

95.362

0.661
  pilT Neisseria meningitidis 8013

63.689

100

0.641
  pilT Neisseria gonorrhoeae MS11

63.401

100

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.299

96.812

0.487
  pilU Pseudomonas stutzeri DSM 10701

40.525

99.42

0.403
  pilU Vibrio cholerae strain A1552

42.154

94.203

0.397
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment