Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R6I08_RS04005 Genome accession   NZ_CP137696
Coordinates   813961..814941 (+) Length   326 a.a.
NCBI ID   WP_098717180.1    Uniprot ID   -
Organism   Escherichia coli isolate FELIX_MS533     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 808961..819941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6I08_RS03975 (R6I08_03975) yggM 809476..810483 (+) 1008 WP_000745217.1 DUF1202 family protein -
  R6I08_RS03980 (R6I08_03980) hemW 810638..811774 (-) 1137 WP_098717179.1 radical SAM family heme chaperone HemW -
  R6I08_RS03985 (R6I08_03985) rdgB 811767..812360 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  R6I08_RS03990 (R6I08_03990) yggU 812368..812658 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  R6I08_RS03995 (R6I08_03995) yggT 812655..813221 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  R6I08_RS04000 (R6I08_04000) yggS 813239..813943 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  R6I08_RS04005 (R6I08_04005) pilT 813961..814941 (+) 981 WP_098717180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R6I08_RS04010 (R6I08_04010) ruvX 815116..815532 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  R6I08_RS04015 (R6I08_04015) yqgE 815532..816095 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  R6I08_RS04020 (R6I08_04020) gshB 816204..817154 (-) 951 WP_000593273.1 glutathione synthase -
  R6I08_RS04025 (R6I08_04025) rsmE 817167..817898 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R6I08_RS04030 (R6I08_04030) endA 817978..818685 (-) 708 WP_000286517.1 deoxyribonuclease I -
  R6I08_RS04035 (R6I08_04035) yggI 818780..819277 (-) 498 WP_032142436.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35931.15 Da        Isoelectric Point: 5.5857

>NTDB_id=899921 R6I08_RS04005 WP_098717180.1 813961..814941(+) (pilT) [Escherichia coli isolate FELIX_MS533]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAVQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLICEGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=899921 R6I08_RS04005 WP_098717180.1 813961..814941(+) (pilT) [Escherichia coli isolate FELIX_MS533]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCATTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGCTGTTGCCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGAAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGCGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGACACCTGGTACTGGCAACCTTACATACACGTGGTGCCGTGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCAGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTTGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362