Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LDO37_RS16405 Genome accession   NZ_AP025144
Coordinates   3647335..3648372 (-) Length   345 a.a.
NCBI ID   WP_101112564.1    Uniprot ID   A0AAV5NWA1
Organism   Vibrio penaeicida strain IFO 15640T     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3642335..3653372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO37_RS16375 - 3642552..3643253 (+) 702 WP_126608178.1 endonuclease -
  LDO37_RS16380 rsmE 3643352..3644083 (+) 732 WP_126608179.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LDO37_RS16385 gshB 3644098..3645048 (+) 951 WP_101112561.1 glutathione synthase -
  LDO37_RS16390 - 3645084..3645647 (+) 564 WP_101112562.1 YqgE/AlgH family protein -
  LDO37_RS16395 ruvX 3645694..3646122 (+) 429 WP_101112582.1 Holliday junction resolvase RuvX -
  LDO37_RS16400 pilU 3646220..3647326 (-) 1107 WP_126608180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LDO37_RS16405 pilT 3647335..3648372 (-) 1038 WP_101112564.1 type IV pilus twitching motility protein PilT Machinery gene
  LDO37_RS16410 - 3648396..3649106 (+) 711 WP_126608181.1 YggS family pyridoxal phosphate-dependent enzyme -
  LDO37_RS16415 proC 3649169..3649987 (+) 819 WP_126608182.1 pyrroline-5-carboxylate reductase -
  LDO37_RS16420 - 3650016..3650573 (+) 558 WP_126608183.1 YggT family protein -
  LDO37_RS16425 yggU 3650573..3650863 (+) 291 WP_126608184.1 DUF167 family protein YggU -
  LDO37_RS16430 - 3650887..3651318 (+) 432 WP_126608185.1 DUF4426 domain-containing protein -
  LDO37_RS16435 - 3651409..3652008 (+) 600 WP_101112570.1 XTP/dITP diphosphatase -
  LDO37_RS16440 hemW 3652015..3653190 (+) 1176 WP_126608186.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37994.16 Da        Isoelectric Point: 6.0472

>NTDB_id=89965 LDO37_RS16405 WP_101112564.1 3647335..3648372(-) (pilT) [Vibrio penaeicida strain IFO 15640T]
MDIAELLDFSVKHNASDLHLSAGVPPMVRVDGDVRKLGVPAFTHADVHRLVFEIMNDAQRAEFEEKLETDFSFELPNVGR
FRVNAFNQSRGSSAVFRTIPTEVPSLEQLDAPDIFTQISNYEKGLVLVTGPTGSGKSTTLAAMVDNINRNHNKHILTIED
PIEFVHQSNKCLINQREVHRDTHSFNAALRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGTDKDMVRSMLSESLRAVISQKLLKRLGGGRAACHEIMLATPAIRNLIREDKVAQMYSVIQTGGAMGMQTTEQHA
KQLIAQGVVDADEVRAKLESDSFGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=89965 LDO37_RS16405 WP_101112564.1 3647335..3648372(-) (pilT) [Vibrio penaeicida strain IFO 15640T]
ATGGATATCGCTGAGTTACTGGATTTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCTGCTGGAGTTCCTCCAAT
GGTGCGTGTAGATGGCGATGTACGTAAGTTAGGTGTCCCTGCTTTTACACACGCCGATGTTCATCGACTGGTCTTTGAAA
TCATGAACGATGCGCAACGAGCAGAGTTTGAAGAAAAGCTGGAAACCGACTTTTCCTTCGAGCTGCCAAATGTCGGGCGT
TTTCGTGTCAATGCGTTTAACCAATCCCGTGGCAGTTCTGCGGTGTTCCGTACGATTCCTACTGAAGTACCTAGCTTGGA
GCAACTTGATGCCCCAGATATCTTTACTCAAATTTCCAATTATGAGAAAGGGCTTGTTCTGGTAACAGGACCAACAGGTT
CAGGTAAGTCAACCACACTGGCAGCCATGGTCGACAATATCAATCGCAACCATAACAAACACATTTTGACGATTGAAGAC
CCGATCGAATTCGTACACCAGAGCAATAAATGTTTGATCAACCAGCGTGAAGTACACCGCGATACACACAGCTTTAATGC
GGCACTTCGAAGCGCACTGCGTGAAGATCCGGATGTGATTCTTGTGGGCGAGCTCCGTGATAAAGAAACCATTAGCTTGG
CATTGACGGCGGCGGAAACCGGACACCTCGTATTTGGCACCTTGCACACCAGTAGCGCGGCGAAAACCGTCGACCGAATC
ATTGATGTGTTCCCGGGTACCGATAAAGACATGGTGCGTTCTATGCTTTCTGAATCATTACGTGCGGTGATTTCGCAAAA
GTTGTTGAAGCGTTTGGGTGGTGGCCGAGCTGCGTGTCACGAAATCATGTTAGCGACACCTGCGATTCGCAACCTTATCC
GTGAAGACAAAGTCGCTCAAATGTATTCCGTGATCCAAACGGGCGGGGCGATGGGAATGCAAACGACAGAGCAACACGCA
AAGCAGCTGATTGCACAAGGTGTGGTAGATGCAGATGAAGTCCGCGCCAAGCTTGAATCAGACTCCTTCGGCTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.377

100

0.864
  pilT Vibrio cholerae strain A1552

86.377

100

0.864
  pilT Acinetobacter baumannii D1279779

71.802

99.71

0.716
  pilT Acinetobacter baumannii strain A118

71.802

99.71

0.716
  pilT Acinetobacter nosocomialis M2

71.512

99.71

0.713
  pilT Acinetobacter baylyi ADP1

70.058

99.71

0.699
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Pseudomonas aeruginosa PAK

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

69.301

95.362

0.661
  pilT Legionella pneumophila strain ERS1305867

69.301

95.362

0.661
  pilT Neisseria meningitidis 8013

63.689

100

0.641
  pilT Neisseria gonorrhoeae MS11

63.401

100

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.299

96.812

0.487
  pilU Pseudomonas stutzeri DSM 10701

40.525

99.42

0.403
  pilU Vibrio cholerae strain A1552

42.154

94.203

0.397
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment