Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R4T22_RS04985 Genome accession   NZ_CP137485
Coordinates   842994..843974 (+) Length   326 a.a.
NCBI ID   WP_020996312.1    Uniprot ID   -
Organism   Citrobacter portucalensis strain F2221     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 837994..848974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4T22_RS04955 (R4T22_04955) ansB 838509..839555 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  R4T22_RS04960 (R4T22_04960) hemW 839670..840806 (-) 1137 WP_020996313.1 radical SAM family heme chaperone HemW -
  R4T22_RS04965 (R4T22_04965) - 840799..841392 (-) 594 WP_003825414.1 XTP/dITP diphosphatase -
  R4T22_RS04970 (R4T22_04970) yggU 841400..841690 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  R4T22_RS04975 (R4T22_04975) - 841687..842253 (-) 567 WP_003825417.1 YggT family protein -
  R4T22_RS04980 (R4T22_04980) - 842272..842976 (-) 705 WP_008785772.1 YggS family pyridoxal phosphate-dependent enzyme -
  R4T22_RS04985 (R4T22_04985) pilT 842994..843974 (+) 981 WP_020996312.1 type IV pilus twitching motility protein PilT Machinery gene
  R4T22_RS04990 (R4T22_04990) ruvX 843971..844387 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  R4T22_RS04995 (R4T22_04995) - 844387..844950 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  R4T22_RS05000 (R4T22_05000) gshB 845127..846074 (-) 948 WP_008785769.1 glutathione synthase -
  R4T22_RS05005 (R4T22_05005) rsmE 846094..846825 (-) 732 WP_008785768.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R4T22_RS05010 (R4T22_05010) endA 846900..847607 (-) 708 WP_008785767.1 deoxyribonuclease I -
  R4T22_RS05015 (R4T22_05015) - 847702..848199 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35994.16 Da        Isoelectric Point: 6.4103

>NTDB_id=898241 R4T22_RS04985 WP_020996312.1 842994..843974(+) (pilT) [Citrobacter portucalensis strain F2221]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTTPDVENLLMCWLSEQQQVQWQEQGQIDFAIALAGSQRLRA
SAFVHQQGTSLALRLLPLDCPRLDDLQTPEAIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQHVARHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAIVRLVDT
FSATEKEPVRNQLADSLRAVLSQKLEEDKLGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=898241 R4T22_RS04985 WP_020996312.1 842994..843974(+) (pilT) [Citrobacter portucalensis strain F2221]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCAGGAACAGGGGCAGATTGATTTTGCTATTGCGCTGGCAGGTTCCCAGCGGCTGCGCGCC
AGTGCTTTTGTTCATCAACAGGGAACCTCACTGGCGCTAAGATTGCTCCCGCTTGATTGTCCACGTTTAGACGATCTCCA
GACTCCCGAGGCCATACCTGAACTGCTGAGGAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTAGAGTACCTTAATCAGCATGTTGCGAGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAACGGGAGGTGGGCGTACACTGCGCTTCTTTTGCCGCCGGTTTACG
CGGCGCGCTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAACTGCGCGACGCGGAAACCATTCGTCTGGCACTAACGG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCACAGGCCATCGTGCGGCTGGTGGACACC
TTTTCGGCAACAGAGAAAGAGCCGGTGCGTAACCAGTTAGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCTGGGAGGACGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTGCCCGGCGTAATTCAAACCGGGCAACAGACAGGTATGCAGACATTTGCACAAAGTCAGCAGCAGCGT
CAGGCGCAGGGACGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

46.667

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.364

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

45.593

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.289

100

0.457
  pilT Acinetobacter baylyi ADP1

45.289

100

0.457
  pilT Acinetobacter baumannii strain A118

44.985

100

0.454
  pilT Acinetobacter baumannii D1279779

44.985

100

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Acinetobacter nosocomialis M2

44.681

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365