Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R6H26_RS22870 Genome accession   NZ_CP137338
Coordinates   4801930..4803045 (-) Length   371 a.a.
NCBI ID   WP_404785833.1    Uniprot ID   -
Organism   Altericista sp. CCNU0014     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4796930..4808045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6H26_RS22845 (R6H26_22685) cdaA 4797709..4798623 (+) 915 WP_404785824.1 diadenylate cyclase CdaA -
  R6H26_RS22850 (R6H26_22690) uppS 4798620..4799372 (+) 753 WP_404785826.1 polyprenyl diphosphate synthase -
  R6H26_RS22855 (R6H26_22695) - 4799402..4799680 (-) 279 WP_404785828.1 DUF3143 domain-containing protein -
  R6H26_RS22860 (R6H26_22700) - 4799840..4800409 (-) 570 WP_404785829.1 J domain-containing protein -
  R6H26_RS22865 (R6H26_22705) - 4800591..4801817 (-) 1227 WP_404785831.1 type II secretion system F family protein -
  R6H26_RS22870 (R6H26_22710) pilT 4801930..4803045 (-) 1116 WP_404785833.1 type IV pilus twitching motility protein PilT Machinery gene
  R6H26_RS22875 (R6H26_22715) pilB/pilB1 4803115..4805115 (-) 2001 WP_404785835.1 GspE/PulE family protein Machinery gene
  R6H26_RS22880 (R6H26_22720) grpE 4805656..4806411 (+) 756 WP_404785837.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40793.69 Da        Isoelectric Point: 6.7638

>NTDB_id=897514 R6H26_RS22870 WP_404785833.1 4801930..4803045(-) (pilT) [Altericista sp. CCNU0014]
MDYMIEDLMEEVVERGGSDLHVSAGLPPYIRVNGKLTPTDRDPLSPEQCQKLIFSMLNNTQRKNLEQNWELDCSYGVRGL
ARFRVNVYKDRGTYAAALRALSSKIPTIETLGLPNIVREMSEKPRGLILVTGPTGSGKSTTLASMINNINMTRAEHILTI
EDPIEFVYEPIKSVIHQRQVGEDTRSFANALRAALREDPDVVLVGELRDLETIQLAITAAETGHLVFGTLHTSSASQTVD
RMVDVFPPEQQTQVRVQLSGSLVAVFSQTLVPRKNVKPGEYGRTLAQEIMVVTPAIANLIREGKTAQIYSAIQTGGKVGM
VTLEKVLADLYRGGLISFESAVSKTSRPDELQRLMGSGPVGAEPVGNQRRH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=897514 R6H26_RS22870 WP_404785833.1 4801930..4803045(-) (pilT) [Altericista sp. CCNU0014]
ATGGACTACATGATTGAAGACTTGATGGAAGAAGTGGTGGAGCGAGGCGGATCCGATCTCCACGTTTCGGCTGGGCTGCC
CCCGTACATTCGAGTCAACGGCAAGCTAACGCCTACAGATCGCGATCCGCTGTCACCGGAGCAGTGCCAAAAACTCATCT
TCAGCATGCTCAACAATACCCAGCGCAAAAACCTAGAGCAAAACTGGGAGCTGGACTGTTCCTACGGCGTGCGCGGTCTA
GCGCGGTTTCGGGTTAATGTCTATAAGGATCGCGGTACCTACGCCGCAGCGTTGCGGGCGCTGAGTTCTAAAATTCCGAC
CATTGAGACATTAGGGCTGCCTAATATCGTTCGGGAGATGTCGGAGAAGCCTAGGGGCTTGATTTTGGTAACGGGCCCTA
CCGGTTCGGGGAAATCTACGACCCTAGCCTCGATGATCAACAACATCAACATGACTCGAGCCGAACACATTCTGACCATT
GAAGATCCCATCGAGTTTGTATACGAACCGATCAAGAGCGTCATCCACCAGCGACAAGTAGGGGAAGACACCCGCAGTTT
TGCCAATGCACTTCGAGCGGCGCTGCGAGAGGATCCCGACGTCGTCCTCGTGGGGGAACTCCGGGACTTAGAAACCATTC
AGCTCGCCATCACCGCCGCCGAAACCGGTCACTTGGTGTTCGGTACCCTACACACCAGTTCCGCCTCTCAAACCGTTGAC
CGGATGGTAGATGTGTTCCCGCCAGAACAGCAAACGCAGGTACGGGTTCAGCTGTCCGGTTCCTTAGTAGCCGTGTTCAG
CCAAACGCTGGTGCCCCGCAAGAACGTGAAGCCTGGAGAGTACGGTCGGACACTGGCCCAAGAAATCATGGTGGTTACAC
CAGCGATCGCCAACCTCATCCGCGAAGGCAAAACAGCGCAAATTTACTCCGCCATTCAAACCGGAGGCAAGGTCGGAATG
GTCACCCTGGAAAAAGTTCTAGCCGATCTCTATCGAGGTGGCCTGATTTCCTTCGAGTCTGCCGTTTCAAAGACTTCTCG
ACCGGATGAGTTGCAGCGCTTAATGGGGTCAGGTCCCGTCGGCGCTGAGCCAGTTGGCAACCAACGACGGCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.179

93.531

0.507
  pilT Pseudomonas stutzeri DSM 10701

53.314

93.531

0.499
  pilT Legionella pneumophila strain Lp02

53.043

92.992

0.493
  pilT Legionella pneumophila strain ERS1305867

53.043

92.992

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.7

96.226

0.488
  pilT Acinetobacter baylyi ADP1

52.161

93.531

0.488
  pilT Acinetobacter baumannii strain A118

51.585

93.531

0.482
  pilT Acinetobacter nosocomialis M2

51.585

93.531

0.482
  pilT Acinetobacter baumannii D1279779

51.585

93.531

0.482
  pilT Neisseria meningitidis 8013

51.632

90.836

0.469
  pilT Neisseria gonorrhoeae MS11

51.335

90.836

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.294

91.644

0.461
  pilT Vibrio cholerae strain A1552

50.294

91.644

0.461
  pilU Pseudomonas stutzeri DSM 10701

40.456

94.609

0.383
  pilU Acinetobacter baylyi ADP1

36.461

100

0.367