Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   R6H26_RS22875 Genome accession   NZ_CP137338
Coordinates   4803115..4805115 (-) Length   666 a.a.
NCBI ID   WP_404785835.1    Uniprot ID   -
Organism   Altericista sp. CCNU0014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4798115..4810115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6H26_RS22850 (R6H26_22690) uppS 4798620..4799372 (+) 753 WP_404785826.1 polyprenyl diphosphate synthase -
  R6H26_RS22855 (R6H26_22695) - 4799402..4799680 (-) 279 WP_404785828.1 DUF3143 domain-containing protein -
  R6H26_RS22860 (R6H26_22700) - 4799840..4800409 (-) 570 WP_404785829.1 J domain-containing protein -
  R6H26_RS22865 (R6H26_22705) - 4800591..4801817 (-) 1227 WP_404785831.1 type II secretion system F family protein -
  R6H26_RS22870 (R6H26_22710) pilT 4801930..4803045 (-) 1116 WP_404785833.1 type IV pilus twitching motility protein PilT Machinery gene
  R6H26_RS22875 (R6H26_22715) pilB/pilB1 4803115..4805115 (-) 2001 WP_404785835.1 GspE/PulE family protein Machinery gene
  R6H26_RS22880 (R6H26_22720) grpE 4805656..4806411 (+) 756 WP_404785837.1 nucleotide exchange factor GrpE -
  R6H26_RS22885 (R6H26_22725) dnaK 4806645..4808618 (+) 1974 WP_404785839.1 molecular chaperone DnaK -
  R6H26_RS22890 (R6H26_22730) dnaJ 4808664..4809803 (+) 1140 WP_404785841.1 molecular chaperone DnaJ -
  R6H26_RS22895 (R6H26_22735) - 4809800..4810066 (+) 267 WP_404785842.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74195.81 Da        Isoelectric Point: 4.7258

>NTDB_id=897515 R6H26_RS22875 WP_404785835.1 4803115..4805115(-) (pilB/pilB1) [Altericista sp. CCNU0014]
MVNSSSSRRALITRQSFSPFGNKLIQTGYIDQEQMLKASVESRQSGKSLVEVLQGLTGRPLPPDLLRQFKQQQLFELKII
YGIDSLDPEVNQIPTDQVGELIERYIPLDVCRTYELIPISLNQEVDPPTLLVAMVNPDNLEAQDRLNRLLRPEGTMLRRM
VIAADDYQQLIQRYLDENAARQAKVEADQMMNIDLADDLENIAGFEELSVDEGETDLGTALQDAETAPIIALVNKVLAKA
LQEGVSDIHIEPQEENLRIRFRKDGVLGYAFPPLPRKIVAAVISRFKIIAELDIAERRQPQDGRIRRLFQGRKVDFRVST
LPSRFGEKVVLRILDNSSTQLGLDKLITDPESLDIVRDMVSRPFGLVLVTGPTGSGKTTTLYSALAECNDPGKNISTAED
PIEYTLPGLTQVQVIRDKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIARLAEM
DVEPFMVSASLIGVVAQRLVRRVCTECRIPYQPSTTDLSRFGLSASSGTEITFYRANTISPEELQTSQSVCIKCNGAGYK
GRVGVYEIMRVTETLQSFITEGAPTERIKEVAVEEGMKTLLAYSLNLVKRGETTLEEVERVTFTDSGLEAELKARRKTSL
TCRVCSADVKQEWLDCPYCMTSRFEE

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=897515 R6H26_RS22875 WP_404785835.1 4803115..4805115(-) (pilB/pilB1) [Altericista sp. CCNU0014]
ATGGTCAACTCTTCTTCTTCGCGGCGTGCCCTGATTACCCGACAAAGCTTCTCTCCCTTTGGGAATAAGCTCATTCAGAC
TGGATATATCGATCAAGAGCAGATGCTCAAGGCTTCTGTAGAAAGCCGCCAGTCGGGCAAGTCGCTGGTTGAGGTCCTTC
AGGGCTTGACCGGTCGGCCATTACCACCGGATTTATTGCGTCAGTTCAAGCAGCAGCAGCTCTTCGAACTCAAAATTATT
TACGGCATTGACTCTCTCGATCCAGAGGTCAACCAAATTCCGACAGATCAGGTGGGCGAGCTCATCGAACGTTACATCCC
GTTAGATGTGTGTCGAACCTACGAATTGATTCCCATTAGCCTCAATCAAGAGGTAGACCCTCCCACCCTATTGGTGGCAA
TGGTCAATCCCGACAATCTAGAGGCGCAGGATCGACTCAATCGGCTTCTGCGCCCCGAAGGCACGATGCTCCGTCGCATG
GTGATTGCGGCAGACGACTACCAGCAATTGATCCAGCGTTACCTGGATGAAAATGCCGCGCGACAGGCAAAGGTCGAAGC
CGATCAGATGATGAATATCGATCTGGCCGACGACCTAGAAAATATTGCTGGGTTTGAAGAGCTATCCGTTGATGAGGGAG
AGACAGACTTAGGGACCGCCCTTCAGGATGCAGAAACAGCCCCCATTATCGCCTTGGTCAATAAGGTTCTGGCAAAAGCC
CTACAGGAAGGGGTCTCCGATATTCACATCGAGCCTCAAGAAGAAAATCTCCGGATTCGCTTTCGCAAAGATGGGGTGTT
GGGCTATGCTTTTCCGCCCTTACCGCGAAAAATTGTGGCGGCGGTGATCTCCAGGTTCAAGATTATTGCGGAGCTGGATA
TTGCAGAGCGACGACAGCCCCAGGACGGTCGGATTCGCCGATTATTCCAGGGACGCAAAGTGGATTTTCGGGTGAGTACG
CTGCCAAGCCGCTTTGGAGAGAAGGTGGTGCTGCGAATTCTCGATAATTCCTCAACCCAGTTGGGATTGGATAAGCTCAT
TACCGATCCAGAGAGCTTGGATATCGTTCGGGATATGGTCAGCCGCCCCTTCGGACTGGTTTTGGTGACGGGGCCGACTG
GCTCTGGGAAAACGACGACACTCTATTCGGCCCTCGCTGAATGTAACGATCCGGGCAAAAACATTAGCACCGCAGAGGAT
CCCATTGAATATACATTGCCCGGACTGACCCAGGTGCAGGTCATTCGGGACAAGGGGATGGATTTTGCCTCTATTTTGAG
AGCGTTTCTGCGACAGGATCCCGATGTCATCCTGGTGGGTGAGACTCGAGATAAGGAGACGGCCAAGACGGCCATTGAGG
CAGCGTTGACGGGACACCTGGTTTTGACAACGCTACACACCAATGACGCCGCCAGCGCGATCGCCCGTCTAGCCGAGATG
GACGTAGAGCCATTCATGGTCTCCGCTTCGTTGATTGGCGTAGTCGCCCAGCGCCTGGTGAGACGAGTCTGTACCGAGTG
TCGCATTCCCTATCAGCCTTCCACGACGGACTTATCCCGATTTGGATTATCTGCCTCGTCGGGGACAGAAATCACTTTTT
ATCGCGCCAACACAATTTCTCCCGAGGAATTGCAAACTAGCCAATCCGTCTGCATCAAATGTAACGGAGCTGGGTACAAA
GGTCGCGTCGGGGTGTACGAAATCATGCGCGTGACCGAAACGCTCCAAAGCTTCATTACTGAGGGTGCGCCTACAGAGCG
CATTAAAGAGGTAGCCGTCGAGGAAGGCATGAAGACGCTATTGGCCTACAGCCTCAATTTAGTCAAACGAGGCGAGACTA
CCCTAGAAGAAGTCGAGCGGGTGACCTTCACGGACTCCGGGCTAGAAGCAGAACTAAAGGCTCGGAGGAAAACATCTTTG
ACCTGCCGCGTTTGCAGCGCAGATGTGAAGCAGGAGTGGCTGGATTGTCCGTACTGCATGACATCCCGATTTGAAGAGTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(235-502)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.663

99.7

0.655