Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R2X24_RS04260 Genome accession   NZ_CP137170
Coordinates   860270..861250 (+) Length   326 a.a.
NCBI ID   WP_275397381.1    Uniprot ID   A0A9X4GJ63
Organism   Citrobacter portucalensis strain F621     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 855270..866250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2X24_RS04230 (R2X24_04230) ansB 855785..856831 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  R2X24_RS04235 (R2X24_04235) hemW 856946..858082 (-) 1137 WP_048236736.1 radical SAM family heme chaperone HemW -
  R2X24_RS04240 (R2X24_04240) - 858075..858668 (-) 594 WP_003825414.1 XTP/dITP diphosphatase -
  R2X24_RS04245 (R2X24_04245) yggU 858676..858966 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  R2X24_RS04250 (R2X24_04250) - 858963..859529 (-) 567 WP_003825417.1 YggT family protein -
  R2X24_RS04255 (R2X24_04255) - 859548..860252 (-) 705 WP_284969307.1 YggS family pyridoxal phosphate-dependent enzyme -
  R2X24_RS04260 (R2X24_04260) pilT 860270..861250 (+) 981 WP_275397381.1 type IV pilus twitching motility protein PilT Machinery gene
  R2X24_RS04265 (R2X24_04265) ruvX 861247..861663 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  R2X24_RS04270 (R2X24_04270) - 861663..862226 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  R2X24_RS04275 (R2X24_04275) gshB 862402..863349 (-) 948 WP_284969306.1 glutathione synthase -
  R2X24_RS04280 (R2X24_04280) rsmE 863369..864100 (-) 732 WP_008785768.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R2X24_RS04285 (R2X24_04285) endA 864175..864882 (-) 708 WP_008785767.1 deoxyribonuclease I -
  R2X24_RS04290 (R2X24_04290) - 864977..865474 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35900.99 Da        Isoelectric Point: 6.3018

>NTDB_id=896673 R2X24_RS04260 WP_275397381.1 860270..861250(+) (pilT) [Citrobacter portucalensis strain F621]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTTPDVENLLMCWLSEQQQVQWREQGQVDFAIALAGSQRLRA
SAFAHQQGTSLALRLLPLDCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQQVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FSATEKEPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=896673 R2X24_RS04260 WP_275397381.1 860270..861250(+) (pilT) [Citrobacter portucalensis strain F621]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGGTAGATTTTGCTATTGCGCTGGCAGGTTCCCAGCGGCTGCGCGCC
AGTGCTTTTGCTCATCAACAGGGAACCTCGCTGGCGCTAAGATTGCTCCCGCTTGATTGCCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTTGAGTACCTTAATCAGCAAGTTGCGGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAACGGGAGGTGGGCGTGCACTGCGCTTCTTTTGCCGCTGGTTTACG
CGGCGCGCTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAGCTACGCGACGTGGAAACCATTCGTCTGGCGTTAACCG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGAGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTTCGGCAACAGAGAAAGAGCCGGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAACAGGGAGGGCGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGCCAGCAGCAGCGT
CAGGCGCAGGGGCGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

46.667

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.364

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

45.593

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.289

100

0.457
  pilT Acinetobacter baumannii D1279779

44.985

100

0.454
  pilT Acinetobacter baumannii strain A118

44.985

100

0.454
  pilT Acinetobacter baylyi ADP1

44.985

100

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Acinetobacter nosocomialis M2

44.681

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368