Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R4540_RS00425 Genome accession   NZ_CP137135
Coordinates   88499..89605 (+) Length   368 a.a.
NCBI ID   WP_000422577.1    Uniprot ID   A0A0H3AMF9
Organism   Vibrio cholerae V52     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 83499..94605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4540_RS00390 (R4540_00390) - 83790..84392 (-) 603 WP_000725014.1 XTP/dITP diphosphatase -
  R4540_RS00395 (R4540_00395) - 84462..84893 (-) 432 WP_001233674.1 DUF4426 domain-containing protein -
  R4540_RS00400 (R4540_00400) yggU 84939..85229 (-) 291 WP_000741914.1 DUF167 family protein YggU -
  R4540_RS00405 (R4540_00405) - 85229..85786 (-) 558 WP_001087261.1 YggT family protein -
  R4540_RS00410 (R4540_00410) proC 85840..86658 (-) 819 WP_000437951.1 pyrroline-5-carboxylate reductase -
  R4540_RS00415 (R4540_00415) - 86711..87421 (-) 711 WP_001256647.1 YggS family pyridoxal phosphate-dependent enzyme -
  R4540_RS00420 (R4540_00420) pilT 87447..88484 (+) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  R4540_RS00425 (R4540_00425) pilU 88499..89605 (+) 1107 WP_000422577.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R4540_RS00430 (R4540_00430) - 89723..90520 (+) 798 WP_001123216.1 helix-turn-helix transcriptional regulator -
  R4540_RS00435 (R4540_00435) tyrS 90629..91909 (+) 1281 WP_001088158.1 tyrosine--tRNA ligase -
  R4540_RS00440 (R4540_00440) ruvX 91947..92369 (-) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  R4540_RS00445 (R4540_00445) - 92450..93013 (-) 564 WP_001054771.1 YqgE/AlgH family protein -
  R4540_RS00450 (R4540_00450) gshB 93049..94005 (-) 957 WP_000593310.1 glutathione synthase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41337.30 Da        Isoelectric Point: 6.5076

>NTDB_id=896416 R4540_RS00425 WP_000422577.1 88499..89605(+) (pilU) [Vibrio cholerae V52]
MELNQYLDSMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=896416 R4540_RS00425 WP_000422577.1 88499..89605(+) (pilU) [Vibrio cholerae V52]
ATGGAGTTGAATCAATATCTGGATAGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAACTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTTGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTACATGAACATAAGCGCTGTATCGTGACGCAGCGAGAAGTCGGGCTGGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACGGGGCATTTGTGTATGGCCACGCTGCACGCCAATAACGCCAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACATGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAAGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3AMF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

99.728

100

0.997
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361