Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R2X26_RS04730 Genome accession   NZ_CP137117
Coordinates   877821..878801 (+) Length   326 a.a.
NCBI ID   WP_071698255.1    Uniprot ID   -
Organism   Citrobacter freundii strain F2119     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 872821..883801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2X26_RS04700 (R2X26_04700) - 873427..874434 (+) 1008 WP_200004640.1 DUF1202 family protein -
  R2X26_RS04705 (R2X26_04705) hemW 874497..875633 (-) 1137 WP_003027105.1 radical SAM family heme chaperone HemW -
  R2X26_RS04710 (R2X26_04710) - 875626..876219 (-) 594 WP_003027104.1 XTP/dITP diphosphatase -
  R2X26_RS04715 (R2X26_04715) yggU 876227..876517 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  R2X26_RS04720 (R2X26_04720) - 876514..877080 (-) 567 WP_003825417.1 YggT family protein -
  R2X26_RS04725 (R2X26_04725) - 877099..877803 (-) 705 WP_071687445.1 YggS family pyridoxal phosphate-dependent enzyme -
  R2X26_RS04730 (R2X26_04730) pilT 877821..878801 (+) 981 WP_071698255.1 type IV pilus twitching motility protein PilT Machinery gene
  R2X26_RS04735 (R2X26_04735) ruvX 878798..879214 (-) 417 WP_003027087.1 Holliday junction resolvase RuvX -
  R2X26_RS04740 (R2X26_04740) - 879214..879777 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  R2X26_RS04745 (R2X26_04745) gshB 879889..880836 (-) 948 WP_003027083.1 glutathione synthase -
  R2X26_RS04750 (R2X26_04750) rsmE 880856..881587 (-) 732 WP_003027080.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R2X26_RS04755 (R2X26_04755) endA 881662..882369 (-) 708 WP_016150969.1 deoxyribonuclease I -
  R2X26_RS04760 (R2X26_04760) - 882464..882961 (-) 498 WP_003838223.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35942.04 Da        Isoelectric Point: 6.4255

>NTDB_id=896302 R2X26_RS04730 WP_071698255.1 877821..878801(+) (pilT) [Citrobacter freundii strain F2119]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRRGKVESAPFTTPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLPDSRRLRA
SAFVHLQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGAHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELFVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=896302 R2X26_RS04730 WP_071698255.1 877821..878801(+) (pilT) [Citrobacter freundii strain F2119]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCCGCGCG
TTGGCGCAGGCGTGGAAAAGTCGAAAGCGCACCGTTTACCACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGCCGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCTGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAATGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGCACACTGCGCCTCTTTCGCCGCCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAAACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTATTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGTTTGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(71-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.009

97.239

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362