Detailed information NTG0893897

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R3J28_RS04615
• Genome accession: NZ_CP136971
• Coordinates: 1021928..1023058 (+)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain Santa29b
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1016928..1028058

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R3J28_RS04595 (R3J28_04585)	-	1017252..1018076 (+)	825	WP_004084943.1	glycosyltransferase family 2 protein	-
R3J28_RS04600 (R3J28_04590)	-	1018693..1019520 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
R3J28_RS04605 (R3J28_04595)	-	1019905..1020117 (-)	213	WP_021358439.1	hypothetical protein	-
R3J28_RS04610 (R3J28_04600)	pilT	1020739..1021773 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
R3J28_RS04615 (R3J28_04605)	pilU	1021928..1023058 (+)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R3J28_RS04620 (R3J28_04610)	-	1023727..1024695 (-)	969	WP_004084952.1	YafY family protein	-
R3J28_RS04625 (R3J28_04615)	-	1025478..1026287 (+)	810	WP_021358437.1	YdiY family protein	-
R3J28_RS04630 (R3J28_04620)	hemC	1026341..1027258 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
R3J28_RS04635 (R3J28_04625)	-	1027266..1028024 (-)	759	WP_004084957.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=893897 R3J28_RS04615 WP_004084950.1 1021928..1023058(+) (pilU) [Xylella fastidiosa subsp. multiplex strain Santa29b]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=893897 R3J28_RS04615 WP_004084950.1 1021928..1023058(+) (pilU) [Xylella fastidiosa subsp. multiplex strain Santa29b]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402