NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	R1T12_RS25315	Genome accession	NZ_CP136909
Coordinates	5418558..5419010 (-)	Length	150 a.a.
NCBI ID	WP_031754077.1	Uniprot ID	P08015
Organism	Pseudomonas aeruginosa PA103
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5413558..5424010

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R1T12_RS25295 (R1T12_25295)	ampD	5414253..5414819 (-)	567	WP_003112844.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
R1T12_RS25300 (R1T12_25300)	-	5414970..5417225 (-)	2256	WP_031754075.1	DUF1631 domain-containing protein	-
R1T12_RS25305 (R1T12_25305)	nadC	5417511..5418359 (+)	849	WP_003161760.1	carboxylating nicotinate-nucleotide diphosphorylase	-
R1T12_RS25315 (R1T12_25315)	pilA	5418558..5419010 (-)	453	WP_031754077.1	pilin	Machinery gene
R1T12_RS25320 (R1T12_25320)	pilB	5419242..5420942 (+)	1701	WP_023094327.1	type IV-A pilus assembly ATPase PilB	Machinery gene
R1T12_RS25325 (R1T12_25325)	pilC	5420946..5422166 (+)	1221	WP_003112840.1	type 4a pilus biogenesis protein PilC	Machinery gene
R1T12_RS25330 (R1T12_25330)	pilD	5422170..5423042 (+)	873	WP_019486071.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
R1T12_RS25335 (R1T12_25335)	coaE	5423039..5423650 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
R1T12_RS25340 (R1T12_25340)	yacG	5423647..5423847 (+)	201	WP_019486072.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15799.09 Da Isoelectric Point: 6.3229

                                >NTDB_id=893382 R1T12_RS25315 WP_031754077.1 5418558..5419010(-) (pilA) [Pseudomonas aeruginosa PA103]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV

GIDEKANKLGTVAVTIKDTGDGTIKFNFATGQSSPKNAGREITLNRTAEGVWTCTSTQEEMFIPKGCNEP

Nucleotide

Download Length: 453 bp

                                >NTDB_id=893382 R1T12_RS25315 WP_031754077.1 5418558..5419010(-) (pilA) [Pseudomonas aeruginosa PA103]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAGCTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT

TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG

AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA

GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAACTATTAAAGACACAGGCGATGGTACTATAAAATTTAA

TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCAGGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT

GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATGAGCCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	P08015

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	65.132	100	0.66
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	46.622	98.667	0.46
pilA	Vibrio cholerae strain A1552	46.622	98.667	0.46
pilA	Vibrio cholerae C6706	46.622	98.667	0.46
pilA/pilAI	Pseudomonas stutzeri DSM 10701	45.333	100	0.453
pilA	Acinetobacter baumannii strain A118	43.919	98.667	0.433
pilA	Vibrio parahaemolyticus RIMD 2210633	41.892	98.667	0.413
comP	Acinetobacter baylyi ADP1	41.333	100	0.413
pilA/pilAII	Pseudomonas stutzeri DSM 10701	42.657	95.333	0.407
pilA/pilA1	Eikenella corrodens VA1	39.189	98.667	0.387
pilA	Haemophilus influenzae 86-028NP	36.667	100	0.367
pilA2	Legionella pneumophila str. Paris	36.735	98	0.36
pilA2	Legionella pneumophila strain ERS1305867	36.735	98	0.36

Detailed information NTG0893382