Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   R1T12_RS25330 Genome accession   NZ_CP136909
Coordinates   5422170..5423042 (+) Length   290 a.a.
NCBI ID   WP_019486071.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa PA103     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5417170..5428042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T12_RS25305 (R1T12_25305) nadC 5417511..5418359 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R1T12_RS25315 (R1T12_25315) pilA 5418558..5419010 (-) 453 WP_031754077.1 pilin Machinery gene
  R1T12_RS25320 (R1T12_25320) pilB 5419242..5420942 (+) 1701 WP_023094327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R1T12_RS25325 (R1T12_25325) pilC 5420946..5422166 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  R1T12_RS25330 (R1T12_25330) pilD 5422170..5423042 (+) 873 WP_019486071.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R1T12_RS25335 (R1T12_25335) coaE 5423039..5423650 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  R1T12_RS25340 (R1T12_25340) yacG 5423647..5423847 (+) 201 WP_019486072.1 DNA gyrase inhibitor YacG -
  R1T12_RS25345 (R1T12_25345) - 5423884..5424093 (-) 210 WP_003094660.1 hypothetical protein -
  R1T12_RS25350 (R1T12_25350) - 5424199..5424888 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  R1T12_RS25355 (R1T12_25355) - 5424885..5425355 (-) 471 WP_019486073.1 hypothetical protein -
  R1T12_RS25360 (R1T12_25360) - 5425352..5425777 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  R1T12_RS25365 (R1T12_25365) - 5425910..5426539 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  R1T12_RS25370 (R1T12_25370) - 5426536..5426985 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  R1T12_RS25375 (R1T12_25375) - 5427011..5427181 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31766.84 Da        Isoelectric Point: 7.7068

>NTDB_id=893385 R1T12_RS25330 WP_019486071.1 5422170..5423042(+) (pilD) [Pseudomonas aeruginosa PA103]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVALHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=893385 R1T12_RS25330 WP_019486071.1 5422170..5423042(+) (pilD) [Pseudomonas aeruginosa PA103]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATACTCCTTGGCCTGCTGGTTGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGTC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTTGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGTCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.714

96.552

0.538
  pilD Vibrio campbellii strain DS40M4

53.004

97.586

0.517
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

48.921

95.862

0.469