Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R1T12_RS25320 Genome accession   NZ_CP136909
Coordinates   5419242..5420942 (+) Length   566 a.a.
NCBI ID   WP_023094327.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa PA103     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5414242..5425942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T12_RS25295 (R1T12_25295) ampD 5414253..5414819 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R1T12_RS25300 (R1T12_25300) - 5414970..5417225 (-) 2256 WP_031754075.1 DUF1631 domain-containing protein -
  R1T12_RS25305 (R1T12_25305) nadC 5417511..5418359 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R1T12_RS25315 (R1T12_25315) pilA 5418558..5419010 (-) 453 WP_031754077.1 pilin Machinery gene
  R1T12_RS25320 (R1T12_25320) pilB 5419242..5420942 (+) 1701 WP_023094327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R1T12_RS25325 (R1T12_25325) pilC 5420946..5422166 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  R1T12_RS25330 (R1T12_25330) pilD 5422170..5423042 (+) 873 WP_019486071.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R1T12_RS25335 (R1T12_25335) coaE 5423039..5423650 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  R1T12_RS25340 (R1T12_25340) yacG 5423647..5423847 (+) 201 WP_019486072.1 DNA gyrase inhibitor YacG -
  R1T12_RS25345 (R1T12_25345) - 5423884..5424093 (-) 210 WP_003094660.1 hypothetical protein -
  R1T12_RS25350 (R1T12_25350) - 5424199..5424888 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  R1T12_RS25355 (R1T12_25355) - 5424885..5425355 (-) 471 WP_019486073.1 hypothetical protein -
  R1T12_RS25360 (R1T12_25360) - 5425352..5425777 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62399.27 Da        Isoelectric Point: 5.7041

>NTDB_id=893383 R1T12_RS25320 WP_023094327.1 5419242..5420942(+) (pilB) [Pseudomonas aeruginosa PA103]
MNDSIQLSGLSRQLVQANLLDEKTALQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
TISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=893383 R1T12_RS25320 WP_023094327.1 5419242..5420942(+) (pilB) [Pseudomonas aeruginosa PA103]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCTCTGCA
GGCGCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGTATCGCCTATTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
ACCATCAGCGAAAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTAGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGACGAATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTGGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.517

100

0.581
  pilB Acinetobacter baylyi ADP1

57.194

99.47

0.569
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

50.887

99.647

0.507
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio cholerae strain A1552

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.645

99.647

0.495
  pilF Thermus thermophilus HB27

40.073

96.996

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.19

92.756

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

38.951

94.346

0.367