Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   R1T12_RS25325 Genome accession   NZ_CP136909
Coordinates   5420946..5422166 (+) Length   406 a.a.
NCBI ID   WP_003112840.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa PA103     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5415946..5427166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T12_RS25305 (R1T12_25305) nadC 5417511..5418359 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R1T12_RS25315 (R1T12_25315) pilA 5418558..5419010 (-) 453 WP_031754077.1 pilin Machinery gene
  R1T12_RS25320 (R1T12_25320) pilB 5419242..5420942 (+) 1701 WP_023094327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R1T12_RS25325 (R1T12_25325) pilC 5420946..5422166 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  R1T12_RS25330 (R1T12_25330) pilD 5422170..5423042 (+) 873 WP_019486071.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R1T12_RS25335 (R1T12_25335) coaE 5423039..5423650 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  R1T12_RS25340 (R1T12_25340) yacG 5423647..5423847 (+) 201 WP_019486072.1 DNA gyrase inhibitor YacG -
  R1T12_RS25345 (R1T12_25345) - 5423884..5424093 (-) 210 WP_003094660.1 hypothetical protein -
  R1T12_RS25350 (R1T12_25350) - 5424199..5424888 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  R1T12_RS25355 (R1T12_25355) - 5424885..5425355 (-) 471 WP_019486073.1 hypothetical protein -
  R1T12_RS25360 (R1T12_25360) - 5425352..5425777 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  R1T12_RS25365 (R1T12_25365) - 5425910..5426539 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  R1T12_RS25370 (R1T12_25370) - 5426536..5426985 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44318.06 Da        Isoelectric Point: 9.8784

>NTDB_id=893384 R1T12_RS25325 WP_003112840.1 5420946..5422166(+) (pilC) [Pseudomonas aeruginosa PA103]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGAIGFTFK
ELHKRSKKFRDTLDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=893384 R1T12_RS25325 WP_003112840.1 5420946..5422166(+) (pilC) [Pseudomonas aeruginosa PA103]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTGAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGTTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCACAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAGAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGTGTGGGGGCGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCGAAAAAATTCCGCGACACACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCTGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baumannii D1279779

60.891

99.507

0.606
  pilC Acinetobacter baylyi ADP1

60.591

100

0.606
  pilC Legionella pneumophila strain ERS1305867

54.545

97.537

0.532
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

41.562

97.783

0.406
  pilC Thermus thermophilus HB27

37.157

98.768

0.367