Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R1C93_RS05555 Genome accession   NZ_CP136754
Coordinates   765378..766358 (+) Length   326 a.a.
NCBI ID   WP_329600986.1    Uniprot ID   -
Organism   Citrobacter koseri strain K820     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 760378..771358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1C93_RS05525 (R1C93_05525) ansB 760889..761935 (+) 1047 WP_049010789.1 L-asparaginase 2 -
  R1C93_RS05530 (R1C93_05530) hemW 762054..763190 (-) 1137 WP_329600985.1 radical SAM family heme chaperone HemW -
  R1C93_RS05535 (R1C93_05535) - 763183..763776 (-) 594 WP_012135070.1 XTP/dITP diphosphatase -
  R1C93_RS05540 (R1C93_05540) yggU 763784..764074 (-) 291 WP_012135069.1 DUF167 family protein YggU -
  R1C93_RS05545 (R1C93_05545) - 764071..764637 (-) 567 WP_012135068.1 YggT family protein -
  R1C93_RS05550 (R1C93_05550) - 764656..765360 (-) 705 WP_049010785.1 YggS family pyridoxal phosphate-dependent enzyme -
  R1C93_RS05555 (R1C93_05555) pilT 765378..766358 (+) 981 WP_329600986.1 type IV pilus twitching motility protein PilT Machinery gene
  R1C93_RS05560 (R1C93_05560) ruvX 766508..766924 (-) 417 WP_115181171.1 Holliday junction resolvase RuvX -
  R1C93_RS05565 (R1C93_05565) - 766924..767487 (-) 564 WP_012135063.1 YqgE/AlgH family protein -
  R1C93_RS05570 (R1C93_05570) gshB 767607..768554 (-) 948 WP_012135062.1 glutathione synthase -
  R1C93_RS05575 (R1C93_05575) rsmE 768567..769298 (-) 732 WP_049010798.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R1C93_RS05580 (R1C93_05580) endA 769376..770083 (-) 708 WP_012135060.1 deoxyribonuclease I -
  R1C93_RS05585 (R1C93_05585) - 770178..770675 (-) 498 WP_071257865.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35613.66 Da        Isoelectric Point: 6.7172

>NTDB_id=892598 R1C93_RS05555 WP_329600986.1 765378..766358(+) (pilT) [Citrobacter koseri strain K820]
MDMEEIVALSVKHNVSDLHLCNAWPARWRKRGKVEIAPFSSPNVEVLLSQWLNDAQRRAWQADGQLDFAVSLPGELRLRA
SAFHHEQGASLALRLLPTTCPQLDALQTPAALPALLGGENGLILVTGATGSGKSTTLAAMVEYLNQHLDGHILTLEDPIE
YRHTSQCCLIQQREVGVHCRSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDS
FPAQEKDPVRSQLAGSLRAVLSQKLETDRQDGRVALFELLINTPAVGNLIREGKIHQLPGVIQTSQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=892598 R1C93_RS05555 WP_329600986.1 765378..766358(+) (pilT) [Citrobacter koseri strain K820]
ATGGATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAACGCATGGCCTGCGCG
CTGGCGTAAACGGGGAAAAGTGGAAATAGCCCCGTTTAGCAGCCCGAATGTCGAGGTTTTGCTGTCACAATGGCTTAACG
ACGCGCAGCGCCGGGCGTGGCAGGCGGATGGTCAGCTCGACTTTGCGGTGTCGCTGCCGGGCGAACTGCGGTTACGCGCC
AGCGCTTTTCATCACGAGCAGGGCGCATCGCTGGCGCTGCGGTTGCTGCCCACTACGTGCCCGCAACTGGACGCGCTCCA
GACGCCTGCCGCGCTACCCGCGCTCCTTGGCGGCGAGAACGGACTGATTCTGGTGACCGGGGCGACGGGAAGCGGGAAAT
CCACCACGCTGGCGGCAATGGTGGAATACCTCAACCAGCATCTCGATGGTCATATCCTGACGCTGGAAGATCCTATTGAG
TACCGCCATACCAGCCAGTGCTGCCTGATTCAGCAGCGGGAGGTTGGCGTACACTGCCGCTCCTTTGCCGCCGGGTTACG
CGCTGCGCTGCGTGAAGACCCGGATGTGATTTTATTAGGCGAACTGCGCGACAGTGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCGACGCTACATACGCGCGGCGCGGCACAGGCGATAGAGCGGCTGGTTGACTCC
TTTCCGGCGCAGGAGAAAGATCCGGTACGCAGTCAACTTGCGGGCAGCCTGCGCGCCGTGTTGTCGCAGAAGCTGGAAAC
CGACAGGCAGGACGGACGCGTGGCGTTATTCGAACTGCTCATCAACACGCCAGCGGTTGGCAATCTTATTCGGGAGGGGA
AAATCCACCAGTTGCCCGGCGTTATTCAGACCAGCCAGCAGGCGGGGATGCAGACGTTTGCCCAGAGTCTGCAACAGCGG
CAGGCGCAGGGCAGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(52-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.376

100

0.515
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.376

100

0.515
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Legionella pneumophila strain ERS1305867

46.894

98.773

0.463
  pilT Legionella pneumophila strain Lp02

46.894

98.773

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Pseudomonas stutzeri DSM 10701

40.909

100

0.414
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

38.58

99.387

0.383