Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RY930_RS04190 Genome accession   NZ_CP136589
Coordinates   889260..890294 (+) Length   344 a.a.
NCBI ID   WP_317114562.1    Uniprot ID   -
Organism   Aeromonas veronii strain BIM B-1812     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 884260..895294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RY930_RS04160 (RY930_04160) - 884917..886113 (+) 1197 WP_157161460.1 NupC/NupG family nucleoside CNT transporter -
  RY930_RS04165 (RY930_04165) - 886215..886634 (-) 420 WP_317114558.1 DUF4426 domain-containing protein -
  RY930_RS04170 (RY930_04170) yggU 886678..886980 (-) 303 WP_317114559.1 DUF167 family protein YggU -
  RY930_RS04175 (RY930_04175) - 886980..887540 (-) 561 WP_317114560.1 YggT family protein -
  RY930_RS04180 (RY930_04180) proC 887552..888376 (-) 825 WP_317114561.1 pyrroline-5-carboxylate reductase -
  RY930_RS04185 (RY930_04185) - 888519..889220 (-) 702 WP_101530187.1 YggS family pyridoxal phosphate-dependent enzyme -
  RY930_RS04190 (RY930_04190) pilT 889260..890294 (+) 1035 WP_317114562.1 type IVa pilus ATPase TapT Machinery gene
  RY930_RS04195 (RY930_04195) pilU 890323..891432 (+) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  RY930_RS04200 (RY930_04200) yaaA 891480..892253 (+) 774 WP_064338818.1 peroxide stress protein YaaA -
  RY930_RS04205 (RY930_04205) - 892448..892873 (+) 426 WP_005340360.1 hypothetical protein -
  RY930_RS04210 (RY930_04210) - 893233..893724 (-) 492 WP_199415370.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  RY930_RS04215 (RY930_04215) arfB 893757..894173 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  RY930_RS04220 (RY930_04220) glnK 894375..894713 (+) 339 WP_005341406.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38258.06 Da        Isoelectric Point: 6.7440

>NTDB_id=891783 RY930_RS04190 WP_317114562.1 889260..890294(+) (pilT) [Aeromonas veronii strain BIM B-1812]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KLLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=891783 RY930_RS04190 WP_317114562.1 889260..890294(+) (pilT) [Aeromonas veronii strain BIM B-1812]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGATTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCTGGCGCGGTATTTCGTACCATCCCCAGCACGGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTACTGGTGACTGGCCCGACCGGTT
CCGGTAAGTCGACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATTCTCACCATCGAAGAT
CCCATCGAATTCGTACACGAGAACAAACGCTGTCTGGTGAACCAGCGGGAAGTACACCGCGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGTGAAATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACTGCCGCCGAAACCGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATTGACGTCTTCCCCGGCGCGGAGAAGGACATGGTGCGTTCCATGCTCTCCGAATCCCTGCGGGCAGTTATCTCCCAGAC
CCTGCTCAAGCGTATTGGCGGTGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTGATCC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTT
AAGCTGCTGGTGAGCCGCGGTGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCCGTCGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Acinetobacter baylyi ADP1

77.259

99.709

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.668

97.965

0.741
  pilT Vibrio cholerae strain A1552

75.668

97.965

0.741
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.609

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407