Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RWT08_RS02500 Genome accession   NZ_CP136247
Coordinates   539036..540166 (-) Length   376 a.a.
NCBI ID   WP_107232233.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain STEN00241     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 534036..545166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RWT08_RS02480 (RWT08_02480) - 534481..535470 (+) 990 WP_249842019.1 fumarylacetoacetate hydrolase family protein -
  RWT08_RS02485 (RWT08_02485) maiA 535485..536210 (+) 726 WP_317657706.1 maleylacetoacetate isomerase -
  RWT08_RS02490 (RWT08_02490) - 536187..537683 (-) 1497 WP_249842017.1 UdgX family uracil-DNA binding protein -
  RWT08_RS02495 (RWT08_02495) - 537664..538947 (-) 1284 WP_249842016.1 putative DNA modification/repair radical SAM protein -
  RWT08_RS02500 (RWT08_02500) pilU 539036..540166 (-) 1131 WP_107232233.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RWT08_RS02505 (RWT08_02505) - 540288..540653 (+) 366 WP_249842015.1 DUF4398 domain-containing protein -
  RWT08_RS02510 (RWT08_02510) - 540650..541534 (+) 885 WP_249842014.1 hypothetical protein -
  RWT08_RS02515 (RWT08_02515) - 541858..542094 (+) 237 WP_057496722.1 YdcH family protein -
  RWT08_RS02520 (RWT08_02520) - 542396..543766 (+) 1371 WP_249842013.1 pyridoxal-phosphate dependent enzyme -
  RWT08_RS02525 (RWT08_02525) - 543890..545074 (+) 1185 WP_249842012.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41626.95 Da        Isoelectric Point: 6.3741

>NTDB_id=889782 RWT08_RS02500 WP_107232233.1 539036..540166(-) (pilU) [Stenotrophomonas maltophilia strain STEN00241]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKSGVIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSSPTQPTITHGF

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=889782 RWT08_RS02500 WP_107232233.1 539036..540166(-) (pilU) [Stenotrophomonas maltophilia strain STEN00241]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCCTGACCACCGGGGCACCGGTCTA
TATCAAGATCGAGGGGAAGCTTTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTGAACATGGCCATTGCCCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCCAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACTCCGCGTGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGATACCCATGCTTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGGAAACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGGTTCACTGGAGGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGAGCGGCGTGATCGAGCAGGAAGAAGCGCTGCGTGCAGCCGATTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCACGATCCGTACGCGGATTTCTCCTCGCCAACCCAGCCCACCATCACCC
ACGGCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.479

0.489
  pilU Acinetobacter baylyi ADP1

49.435

94.149

0.465
  pilU Vibrio cholerae strain A1552

49.153

94.149

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.81

95.213

0.436
  pilT Pseudomonas aeruginosa PAK

42.899

89.894

0.386
  pilT Acinetobacter baumannii strain A118

42.012

89.894

0.378
  pilT Acinetobacter nosocomialis M2

42.012

89.894

0.378
  pilT Acinetobacter baumannii D1279779

42.012

89.894

0.378
  pilT Acinetobacter baylyi ADP1

41.471

90.426

0.375
  pilT Pseudomonas stutzeri DSM 10701

41.42

89.894

0.372
  pilT Legionella pneumophila strain Lp02

40.533

89.894

0.364
  pilT Legionella pneumophila strain ERS1305867

40.533

89.894

0.364
  pilT Vibrio cholerae strain A1552

41.141

88.564

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.564

0.364
  pilT Neisseria gonorrhoeae MS11

39.193

92.287

0.362
  pilT Neisseria meningitidis 8013

39.193

92.287

0.362