Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RND74_RS17155 Genome accession   NZ_CP136135
Coordinates   3788097..3789113 (-) Length   338 a.a.
NCBI ID   WP_406641395.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain ZRIMU1702DQ     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3783097..3794113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RND74_RS17140 nsrR 3783444..3783869 (-) 426 WP_014916532.1 nitric oxide-sensing transcriptional repressor NsrR -
  RND74_RS17145 - 3784197..3785768 (+) 1572 WP_406641392.1 methyl-accepting chemotaxis protein -
  RND74_RS17150 - 3786296..3788128 (+) 1833 WP_039552866.1 diguanylate cyclase -
  RND74_RS17155 pilT 3788097..3789113 (-) 1017 WP_406641395.1 type IV pilus twitching motility protein PilT Machinery gene
  RND74_RS17160 - 3789134..3789847 (+) 714 WP_180788544.1 YggS family pyridoxal phosphate-dependent enzyme -
  RND74_RS17165 proC 3789942..3790763 (+) 822 WP_203450575.1 pyrroline-5-carboxylate reductase -
  RND74_RS17170 - 3790812..3791366 (+) 555 WP_010279107.1 YggT family protein -
  RND74_RS17175 yggU 3791363..3791653 (+) 291 WP_406641398.1 DUF167 family protein YggU -
  RND74_RS17180 rdgB 3791671..3792264 (+) 594 WP_406641400.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  RND74_RS17185 hemW 3792257..3793399 (+) 1143 WP_406641402.1 radical SAM family heme chaperone HemW -
  RND74_RS17190 - 3793628..3793888 (+) 261 WP_039558289.1 type II toxin-antitoxin system ParD family antitoxin -
  RND74_RS17195 - 3793951..3794070 (+) 120 WP_406641403.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36693.89 Da        Isoelectric Point: 6.4951

>NTDB_id=888800 RND74_RS17155 WP_406641395.1 3788097..3789113(-) (pilT) [Pectobacterium brasiliense strain ZRIMU1702DQ]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLQQVGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLATLQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSERHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=888800 RND74_RS17155 WP_406641395.1 3788097..3789113(-) (pilT) [Pectobacterium brasiliense strain ZRIMU1702DQ]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCGGCTCAGAGAGAGCAACTGCAGCAGGTTGGGCAGGTGGATGGGGCATTAATGCTGCCTGATGGACAA
CGCCTGCGGGTGAATGTATTTCGCCAGCGTGAAGGGCTGTCTGCGGCGCTGCGTATTATTCCGTCCGCTCAGCCTTCATT
GGCGACGCTACAGGCACCACCCGTTCTCTCGACGCTACTGGAGAAGCCGAACGGACTAATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGCGAGCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGCGGGAGATCGGTGCGCACAGTGCGTCCTTTGC
GCAGGCGCTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGCGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTTACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCGGTTGACCGT
CTGATTGATGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTAGCTACCTGTTTGCAGGCCGTGGTGACGCA
AAGGCTGTTGCCCGCTGCGCAAGGTGGCCGAATTGCGCTTTATGAAGTGCTGACGGCGACGGCGGCTGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.212

97.633

0.5
  pilT Vibrio cholerae strain A1552

51.212

97.633

0.5
  pilT Neisseria meningitidis 8013

50.602

98.225

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

98.225

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.64

97.929

0.476
  pilT Acinetobacter baumannii strain A118

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

99.704

0.423
  pilU Vibrio cholerae strain A1552

39.067

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.64

98.521

0.391
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367