Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RND66_RS17725 Genome accession   NZ_CP136134
Coordinates   4010046..4011062 (-) Length   338 a.a.
NCBI ID   WP_205594628.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain ZRIMU1701SM     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4005046..4016062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RND66_RS17710 nsrR 4005378..4005803 (-) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -
  RND66_RS17715 - 4006147..4007718 (+) 1572 WP_205564594.1 methyl-accepting chemotaxis protein -
  RND66_RS17720 - 4008245..4010077 (+) 1833 WP_104212826.1 diguanylate cyclase -
  RND66_RS17725 pilT 4010046..4011062 (-) 1017 WP_205594628.1 type IV pilus twitching motility protein PilT Machinery gene
  RND66_RS17730 - 4011083..4011796 (+) 714 WP_039517028.1 YggS family pyridoxal phosphate-dependent enzyme -
  RND66_RS17735 proC 4011891..4012712 (+) 822 WP_039498040.1 pyrroline-5-carboxylate reductase -
  RND66_RS17740 - 4012761..4013315 (+) 555 WP_010295003.1 YggT family protein -
  RND66_RS17745 yggU 4013312..4013602 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  RND66_RS17750 - 4013620..4014213 (+) 594 WP_014916538.1 XTP/dITP diphosphatase -
  RND66_RS17755 hemW 4014206..4015348 (+) 1143 WP_205594629.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36528.71 Da        Isoelectric Point: 6.8191

>NTDB_id=888777 RND66_RS17725 WP_205594628.1 4010046..4011062(-) (pilT) [Pectobacterium brasiliense strain ZRIMU1701SM]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLAPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLAALQAPPVLSTLLEKPNGLILVTGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
NQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=888777 RND66_RS17725 WP_205594628.1 4010046..4011062(-) (pilT) [Pectobacterium brasiliense strain ZRIMU1701SM]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GCTACGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGCACCCGCTCAGCGCGAGCAACTGCGGCAGGCTGGTCAGGTGGACGGCGCGCTAATGCTGCCCGATGGACAA
CGCCTGCGGGTCAATGTATTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTACGTATTATCCCGTCCGCTCAGCCTTCATT
GGCGGCGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGACTGATCCTAGTTACCGGTGCAACGG
GGAGCGGTAAATCTACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGAGGGAGATTGGTGCACACAGTGCGTCCTTTGC
GCAGGCGCTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGGGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTAGCTACCTGCTTGCAGGCCGTGGTGACGCA
AAGGCTGTTGCCTGCTGCGCAAGGTGGCCGAATTGCGCTTTATGAAGTGCTGACGGCGACGGCGGCGGTCAGTAACCTGA
TTAGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
AACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

97.633

0.503
  pilT Vibrio cholerae strain A1552

51.515

97.633

0.503
  pilT Neisseria meningitidis 8013

50.602

98.225

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

98.225

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.547

97.929

0.485
  pilT Pseudomonas aeruginosa PAK

49.245

97.929

0.482
  pilT Legionella pneumophila strain Lp02

49.245

97.929

0.482
  pilT Legionella pneumophila strain ERS1305867

49.245

97.929

0.482
  pilT Acinetobacter baylyi ADP1

49.245

97.929

0.482
  pilT Acinetobacter nosocomialis M2

48.943

97.929

0.479
  pilT Acinetobacter baumannii D1279779

48.943

97.929

0.479
  pilT Acinetobacter baumannii strain A118

48.943

97.929

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.73

99.704

0.426
  pilU Vibrio cholerae strain A1552

39.359

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.498

94.97

0.385
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367