Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RS893_RS07200 Genome accession   NZ_CP135918
Coordinates   1730703..1731830 (+) Length   375 a.a.
NCBI ID   WP_315791901.1    Uniprot ID   -
Organism   Fischerella sp. JS2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1725703..1736830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS893_RS07190 grpE 1727120..1727860 (-) 741 WP_315791900.1 nucleotide exchange factor GrpE -
  RS893_RS07195 pilB/pilB1 1728578..1730590 (+) 2013 WP_315790520.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  RS893_RS07200 pilT 1730703..1731830 (+) 1128 WP_315791901.1 type IV pilus twitching motility protein PilT Machinery gene
  RS893_RS07205 - 1731948..1733168 (+) 1221 WP_315790522.1 type II secretion system F family protein -
  RS893_RS07210 - 1733263..1733811 (+) 549 WP_315790524.1 hypothetical protein -
  RS893_RS07215 - 1734106..1734306 (-) 201 WP_315791902.1 type II toxin-antitoxin system RelE/ParE family toxin -
  RS893_RS07220 - 1734342..1734530 (-) 189 WP_315790525.1 hypothetical protein -
  RS893_RS07225 - 1734841..1735269 (+) 429 WP_315791903.1 EH signature domain-containing protein -
  RS893_RS07230 - 1735498..1736520 (-) 1023 WP_315790526.1 saccharopine dehydrogenase-like oxidoreductase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 40982.37 Da        Isoelectric Point: 7.1091

>NTDB_id=887643 RS893_RS07200 WP_315791901.1 1730703..1731830(+) (pilT) [Fischerella sp. JS2]
MEMMIEDLMEQMIEMGGSDMHLSAGLPPYFRISGKLTPIGEEPLSGDQCQRLIFSMLNNSQRKTLEQNWELDCSYGVKGL
ARFRVNVYKERGSYAACLRALSSKIPNFDKLGLPDVVREMSEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPIEFVYEPIKSLIHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHEKQTQVRVQLSNSLVAVFSQTLVPKKNPKAGEFGRVMAQEIMIVTPAISNLIREGKTAQIYSSIQTGGKLGM
QTLEKVLADLYKVGTISFEAAMSKTSKPDEVQRLIGPAAPPAAGKVGATGAIKTH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=887643 RS893_RS07200 WP_315791901.1 1730703..1731830(+) (pilT) [Fischerella sp. JS2]
ATGGAAATGATGATTGAAGATTTAATGGAGCAAATGATTGAAATGGGTGGTTCGGATATGCATTTATCTGCTGGTTTGCC
CCCCTATTTTCGCATTAGTGGTAAATTAACACCCATTGGCGAAGAGCCTTTATCTGGGGATCAGTGCCAAAGGTTAATTT
TCAGTATGCTCAATAACTCCCAACGAAAAACATTAGAGCAAAACTGGGAGTTAGATTGTTCTTATGGTGTTAAGGGATTA
GCACGTTTCCGCGTTAATGTTTATAAAGAACGCGGTTCTTATGCAGCTTGTTTGCGGGCATTAAGTTCTAAAATACCTAA
CTTTGATAAATTAGGTTTGCCCGATGTAGTACGAGAAATGTCGGAAAAGCCCAGGGGATTAATTCTAGTTACAGGCCCTA
CTGGTTCTGGAAAAACAACTACTCTAGCGGCAATGATTGATTTAATTAACCGCACACGCGCAGAACATATATTGACAGTA
GAAGATCCGATAGAGTTTGTTTATGAACCAATCAAAAGTCTAATTCATCAAAGGCAATTGGGTGAAGATACAAAGAGTTT
TGCTAATGCTTTGAAAGCCGCTCTTAGAGAAGATCCAGATATTATTCTGGTAGGAGAAATGCGGGACTTAGAAACTATTT
CTTTAGCTATTTCTGCTGCTGAAACTGGTCACTTAGTATTTGGTACTTTACATACTAGTTCGGCAGCACAAACTGTTGAC
CGAATTATTGACGTTTTTCCCCATGAAAAACAAACTCAAGTACGAGTACAGTTATCTAACTCGCTAGTAGCAGTATTTAG
CCAAACTTTAGTTCCAAAAAAGAATCCCAAAGCAGGTGAATTTGGTCGAGTAATGGCACAGGAAATAATGATAGTTACTC
CAGCTATTTCTAACTTGATTCGTGAAGGCAAAACAGCACAAATTTACTCATCTATTCAGACAGGTGGTAAATTAGGTATG
CAAACTCTGGAGAAGGTTTTAGCTGATTTATATAAAGTAGGAACTATTTCTTTTGAAGCAGCAATGTCTAAAACTTCTAA
GCCAGATGAAGTGCAACGTCTTATTGGTCCTGCTGCACCACCTGCTGCCGGAAAAGTCGGTGCAACTGGGGCTATAAAAA
CACATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.043

92.533

0.509
  pilT Pseudomonas stutzeri DSM 10701

54.467

92.533

0.504
  pilT Legionella pneumophila strain Lp02

53.89

92.533

0.499
  pilT Acinetobacter baylyi ADP1

53.89

92.533

0.499
  pilT Legionella pneumophila strain ERS1305867

53.89

92.533

0.499
  pilT Acinetobacter baumannii strain A118

52.45

92.533

0.485
  pilT Acinetobacter nosocomialis M2

52.45

92.533

0.485
  pilT Acinetobacter baumannii D1279779

52.45

92.533

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.509

98.4

0.477
  pilT Neisseria gonorrhoeae MS11

51.032

90.4

0.461
  pilT Neisseria meningitidis 8013

50.737

90.4

0.459
  pilT Vibrio cholerae strain A1552

50

91.2

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

91.2

0.456
  pilU Acinetobacter baylyi ADP1

38.527

94.133

0.363
  pilU Pseudomonas stutzeri DSM 10701

38.527

94.133

0.363