Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RS893_RS07195 Genome accession   NZ_CP135918
Coordinates   1728578..1730590 (+) Length   670 a.a.
NCBI ID   WP_315790520.1    Uniprot ID   -
Organism   Fischerella sp. JS2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1723578..1735590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS893_RS07185 dnaK 1724846..1726807 (-) 1962 WP_315790519.1 molecular chaperone DnaK -
  RS893_RS07190 grpE 1727120..1727860 (-) 741 WP_315791900.1 nucleotide exchange factor GrpE -
  RS893_RS07195 pilB/pilB1 1728578..1730590 (+) 2013 WP_315790520.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  RS893_RS07200 pilT 1730703..1731830 (+) 1128 WP_315791901.1 type IV pilus twitching motility protein PilT Machinery gene
  RS893_RS07205 - 1731948..1733168 (+) 1221 WP_315790522.1 type II secretion system F family protein -
  RS893_RS07210 - 1733263..1733811 (+) 549 WP_315790524.1 hypothetical protein -
  RS893_RS07215 - 1734106..1734306 (-) 201 WP_315791902.1 type II toxin-antitoxin system RelE/ParE family toxin -
  RS893_RS07220 - 1734342..1734530 (-) 189 WP_315790525.1 hypothetical protein -
  RS893_RS07225 - 1734841..1735269 (+) 429 WP_315791903.1 EH signature domain-containing protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74787.53 Da        Isoelectric Point: 5.1771

>NTDB_id=887642 RS893_RS07195 WP_315790520.1 1728578..1730590(+) (pilB/pilB1) [Fischerella sp. JS2]
MTNSSPQRRSTALSTRAEFSPFGNKLVQSGFVNSEQMRQALIESRKSGIPLIEVLESVSGRQLSPELLRQYKKQQLFELK
ILYGVESLDPEISSVGSTTVAHLIDTLIPVDVCRRHRLVPLSKNEDQNPPSVLVAMVDPDNLEASDDLNRILRPQGLTLQ
RMVITQEDYQLLINVYLNDLAVRQKHLEQEKYTDINQDLENLENLNLNDAPEESEADLGAAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIIANLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSATQLGLDKLITDPETLHIVQEMVSRPFGLILVTGPTGSGKTTSLYSALAERNEPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCPDCRIPYTPTVEELARYGLSASQEVEVTFYKANTLTTEEIQAAKTQGHICPTCNG
VGYKGRCGVYEVMRISESLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGSTTLEEVERVTFTDTGLEAELKAKR
KTSLTCRTCNAGLRPEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=887642 RS893_RS07195 WP_315790520.1 1728578..1730590(+) (pilB/pilB1) [Fischerella sp. JS2]
ATGACTAACTCGTCACCACAACGGCGTAGTACAGCTCTATCTACCAGAGCAGAATTTTCACCCTTTGGTAACAAACTTGT
ACAATCTGGCTTTGTCAACAGCGAACAAATGAGACAGGCGCTAATTGAAAGCCGCAAGTCTGGTATCCCTTTAATAGAAG
TACTCGAATCAGTATCGGGGCGACAATTATCACCAGAGTTACTCAGGCAGTACAAAAAACAGCAGTTATTTGAACTTAAA
ATACTTTACGGCGTAGAATCCCTCGATCCGGAAATTAGTTCGGTAGGTAGCACCACAGTAGCCCACCTGATTGATACCCT
CATTCCAGTAGATGTTTGTCGTCGTCACCGCTTAGTACCACTGTCGAAAAACGAGGATCAAAACCCGCCCTCTGTGCTAG
TGGCGATGGTTGATCCAGATAATCTAGAAGCTTCAGATGACTTAAACCGGATTCTGCGCCCCCAAGGCTTGACCTTGCAG
CGCATGGTAATTACTCAAGAAGATTATCAACTTTTAATCAACGTATATCTGAATGATTTAGCTGTTCGGCAAAAGCATTT
AGAACAAGAAAAATATACAGACATCAATCAAGATTTAGAAAATCTAGAAAATCTTAACCTAAATGACGCTCCAGAAGAAT
CCGAAGCTGACTTAGGGGCGGCGATGAAGGGTGCTGAAGATGCACCTGTGATCAATCTGGTGAACAGAATCTTGGCTAAA
GCACTGCATGAGAAAGTATCTGATATTCATATAGAACCACAAGAAGAAAACCTCCGCATTCGCTTTCGTAAAGATGGGGT
TTTGCGGGAGGCTTTCGACCCTTTGCCAAAAAAAATCATTCCTGCTGTGACAGCGCGGTTTAAAATCATTGCCAATTTAG
ATATTGCTGAACGACGCTTACCTCAAGACGGACGGATTAGACGCTTGTTCGAGGGGCGTAAAGTTGACTTCCGTGTAAAT
ACTTTACCCAGTCGCTACGGGGAAAAAGTAGTACTACGGATTTTGGATAACTCTGCCACGCAACTCGGTTTAGATAAGCT
AATTACCGATCCAGAGACGTTGCATATTGTTCAAGAAATGGTTAGCCGTCCCTTTGGCTTAATTTTAGTAACAGGACCGA
CAGGTTCTGGTAAGACAACTTCGCTGTATTCAGCGCTAGCAGAAAGAAACGAGCCGGGAATTAATATCAGTACTGTAGAA
GATCCAATTGAGTACAGTTTGCCGGGAATTACTCAAGTACAGGTAATTCGGGAAAAAGGGTTAGATTTTGCAACGGCATT
AAGGGCATTTTTGCGACAAGATCCAGATGTGCTACTCGTAGGTGAAACACGGGATAAAGAAACAGCAAAAACGGCGATTG
AAGCAGCATTAACTGGTCACTTAGTATTAACTACTTTACATACTAATGATGCTCCTGGTGCGATCGCTCGTTTAGGAGAA
ATGGGTATTGAACCTTTCATGGTTTCTAGTTCATTGATTGGTGTCTTGGCCCAGCGTTTGGTACGGCGTGTGTGTCCTGA
CTGTCGCATTCCCTACACACCCACAGTAGAAGAACTCGCCCGTTATGGTTTGTCGGCTTCCCAAGAAGTAGAAGTAACCT
TTTATAAAGCTAATACTCTGACCACAGAGGAAATTCAAGCAGCCAAAACTCAAGGTCATATTTGTCCCACCTGTAATGGC
GTAGGCTACAAAGGACGTTGTGGTGTCTATGAGGTAATGCGAATCAGTGAAAGTCTGCAAACCCTCATTAACGAAGAAGC
ACCGACAGAACGCATCAAAGAAGTAGCAGTAGAAGAGGGTATGAAAACCTTGCTGGCCTACAGCTTGGACTTAGTACGTC
AAGGTTCAACCACCCTAGAAGAGGTAGAACGAGTCACCTTTACTGACACAGGCTTAGAAGCAGAACTGAAAGCCAAACGC
AAGACTAGTCTGACTTGCCGGACTTGTAATGCGGGGTTGCGGCCAGAATGGTTGGATTGTCCGTATTGCATGACGCCTCG
TTTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.923

100

0.661
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.098

91.194

0.366
  pilB Vibrio parahaemolyticus RIMD 2210633

39.737

90.896

0.361