Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LDN81_RS22130 Genome accession   NZ_AP024847
Coordinates   4589493..4590515 (-) Length   340 a.a.
NCBI ID   WP_041036668.1    Uniprot ID   A0AAT9EHZ0
Organism   Serratia marcescens strain 2020-O-9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4584493..4595515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDN81_RS22100 (SMGES_43840) - 4585297..4585809 (+) 513 WP_033654666.1 SprT family zinc-dependent metalloprotease -
  LDN81_RS22105 (SMGES_43850) endA 4585911..4586606 (+) 696 WP_041036666.1 deoxyribonuclease I -
  LDN81_RS22110 (SMGES_43860) rsmE 4586676..4587407 (+) 732 WP_033654665.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LDN81_RS22115 (SMGES_43870) gshB 4587418..4588368 (+) 951 WP_033654664.1 glutathione synthase -
  LDN81_RS22120 (SMGES_43880) - 4588511..4589074 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  LDN81_RS22125 (SMGES_43890) ruvX 4589074..4589496 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  LDN81_RS22130 (SMGES_43900) pilT 4589493..4590515 (-) 1023 WP_041036668.1 type IV pilus twitching motility protein PilT Machinery gene
  LDN81_RS22135 (SMGES_43920) - 4590536..4591243 (+) 708 WP_041036670.1 YggS family pyridoxal phosphate-dependent enzyme -
  LDN81_RS22140 (SMGES_43930) proC 4591263..4592084 (+) 822 WP_041036672.1 pyrroline-5-carboxylate reductase -
  LDN81_RS22145 (SMGES_43940) - 4592116..4592670 (+) 555 WP_033635944.1 YggT family protein -
  LDN81_RS22150 (SMGES_43950) yggU 4592667..4592957 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  LDN81_RS22155 (SMGES_43960) - 4592975..4593568 (+) 594 WP_033635946.1 XTP/dITP diphosphatase -
  LDN81_RS22160 (SMGES_43970) hemW 4593561..4594703 (+) 1143 WP_041036677.1 radical SAM family heme chaperone HemW -
  LDN81_RS22165 - 4594740..4594826 (-) 87 Protein_4333 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36430.69 Da        Isoelectric Point: 6.8881

>NTDB_id=88726 LDN81_RS22130 WP_041036668.1 4589493..4590515(-) (pilT) [Serratia marcescens strain 2020-O-9]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQIDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGESAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=88726 LDN81_RS22130 WP_041036668.1 4589493..4590515(-) (pilT) [Serratia marcescens strain 2020-O-9]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGATCGATCTGGCGCTGCACAGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAACTGCGCGATATCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTTAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGAGTGCGGGGGAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Neisseria gonorrhoeae MS11

49.851

98.529

0.491
  pilT Pseudomonas aeruginosa PAK

49.555

99.118

0.491
  pilT Neisseria meningitidis 8013

50.453

97.353

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.961

99.118

0.485
  pilT Legionella pneumophila strain ERS1305867

49.398

97.647

0.482
  pilT Legionella pneumophila strain Lp02

49.398

97.647

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.82

100

0.459
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371

Multiple sequence alignment