Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RS400_RS02710 Genome accession   NZ_CP135602
Coordinates   587738..588865 (-) Length   375 a.a.
NCBI ID   WP_099596198.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain SI1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 582738..593865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS400_RS02690 (RS400_02690) - 583166..584155 (+) 990 WP_099596200.1 fumarylacetoacetate hydrolase family protein -
  RS400_RS02695 (RS400_02695) maiA 584170..584838 (+) 669 WP_065183729.1 maleylacetoacetate isomerase -
  RS400_RS02700 (RS400_02700) - 584883..586343 (-) 1461 WP_099596199.1 UdgX family uracil-DNA binding protein -
  RS400_RS02705 (RS400_02705) - 586324..587607 (-) 1284 WP_185695421.1 putative DNA modification/repair radical SAM protein -
  RS400_RS02710 (RS400_02710) pilU 587738..588865 (-) 1128 WP_099596198.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RS400_RS02715 (RS400_02715) - 588987..589352 (+) 366 WP_024957357.1 DUF4398 domain-containing protein -
  RS400_RS02720 (RS400_02720) - 589349..590233 (+) 885 WP_005407962.1 hypothetical protein -
  RS400_RS02725 (RS400_02725) - 590558..590794 (+) 237 WP_005407964.1 YdcH family protein -
  RS400_RS02730 (RS400_02730) - 591095..592465 (+) 1371 WP_005412160.1 pyridoxal-phosphate dependent enzyme -
  RS400_RS02735 (RS400_02735) - 592589..593773 (+) 1185 WP_099596196.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41425.73 Da        Isoelectric Point: 6.3741

>NTDB_id=887140 RS400_RS02710 WP_099596198.1 587738..588865(-) (pilU) [Stenotrophomonas maltophilia strain SI1]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLLNTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKAGTIEQEEALRAADSRDGLALKFRLSEGSNGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=887140 RS400_RS02710 WP_099596198.1 587738..588865(-) (pilU) [Stenotrophomonas maltophilia strain SI1]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGTCGC
TTCCGCGTCAATGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTCATCCGTGCCATCCGCAGCCGGATTCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCACGCGGGCTGGTACTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAAGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGATACCCATGCCTTCCACAA
CGCGCTGAAGAACGCCATGCGTGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCAGAGACGATGGAAGCGG
CGATCGCCTTTGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTGAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGCCGTCGCCTGCCGGCCACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTGCACGAAATCAAGGCGGCGATGGAAGCATCGCTGGAAGAGGGCATGGAGAGTTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCACCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCAACGGCGAGCATGACCCGTACGCGGATTTCTCGGCCGGCGCACCCAGCATCACCCACG
GTTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.975

95.733

0.488
  pilU Acinetobacter baylyi ADP1

49.02

95.2

0.467
  pilU Vibrio cholerae strain A1552

49.157

94.933

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.04

99.467

0.448
  pilT Pseudomonas aeruginosa PAK

42.604

90.133

0.384
  pilT Acinetobacter baumannii strain A118

41.42

90.133

0.373
  pilT Acinetobacter nosocomialis M2

41.42

90.133

0.373
  pilT Acinetobacter baumannii D1279779

41.42

90.133

0.373
  pilT Acinetobacter baylyi ADP1

41.176

90.667

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.124

90.133

0.371
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

41.141

88.8

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.8

0.365
  pilT Neisseria gonorrhoeae MS11

38.905

92.533

0.36
  pilT Neisseria meningitidis 8013

38.905

92.533

0.36