Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RQP59_RS19550 Genome accession   NZ_CP135253
Coordinates   4020555..4021535 (-) Length   326 a.a.
NCBI ID   WP_265194864.1    Uniprot ID   A0AA96M0D2
Organism   Enterobacter chuandaensis strain FAHZZU5885     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4015555..4026535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RQP59_RS19520 (RQP59_19495) - 4016444..4016941 (+) 498 WP_265194862.1 SprT family zinc-dependent metalloprotease -
  RQP59_RS19525 (RQP59_19500) endA 4017036..4017743 (+) 708 WP_080330045.1 deoxyribonuclease I -
  RQP59_RS19530 (RQP59_19505) rsmE 4017795..4018526 (+) 732 WP_041908327.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RQP59_RS19535 (RQP59_19510) gshB 4018546..4019493 (+) 948 WP_033146605.1 glutathione synthase -
  RQP59_RS19540 (RQP59_19515) - 4019568..4020128 (+) 561 WP_265194863.1 YqgE/AlgH family protein -
  RQP59_RS19545 (RQP59_19520) ruvX 4020128..4020544 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  RQP59_RS19550 (RQP59_19525) pilT 4020555..4021535 (-) 981 WP_265194864.1 type IV pilus twitching motility protein PilT Machinery gene
  RQP59_RS19555 (RQP59_19530) - 4021553..4022254 (+) 702 WP_265194865.1 YggS family pyridoxal phosphate-dependent enzyme -
  RQP59_RS19560 (RQP59_19535) - 4022276..4022842 (+) 567 WP_080330042.1 YggT family protein -
  RQP59_RS19565 (RQP59_19540) yggU 4022839..4023135 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  RQP59_RS19570 (RQP59_19545) - 4023139..4023732 (+) 594 WP_048979374.1 XTP/dITP diphosphatase -
  RQP59_RS19575 (RQP59_19550) hemW 4023725..4024867 (+) 1143 WP_265194866.1 radical SAM family heme chaperone HemW -
  RQP59_RS19580 (RQP59_19555) - 4024915..4025031 (+) 117 Protein_3830 DUF559 domain-containing protein -
  RQP59_RS19585 (RQP59_19560) - 4025107..4025823 (-) 717 WP_080330040.1 DUF2884 domain-containing protein -
  RQP59_RS19590 (RQP59_19565) - 4025881..4026207 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35531.62 Da        Isoelectric Point: 6.4994

>NTDB_id=886312 RQP59_RS19550 WP_265194864.1 4020555..4021535(-) (pilT) [Enterobacter chuandaensis strain FAHZZU5885]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGNQRLRG
SAFKQMKGVSVTLRLLPRSCPQLASLGVPRALPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNRQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRGALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQVGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=886312 RQP59_RS19550 WP_265194864.1 4020555..4021535(-) (pilT) [Enterobacter chuandaensis strain FAHZZU5885]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGACTAGGCCGCCTTGAACCGGCCCCGTTTCCGCCGCCTGACGTCGGGGCGTTATTAAAGGCGTGGCTGAACG
ACGAGCAGCAGGGAGCCTGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCGCAGGAAACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCGTCTCCGTTACCCTGCGCCTGTTGCCGCGTTCCTGTCCGCAGCTCGCTTCGCTGGG
TGTGCCGCGGGCGCTTCCCGAACTGTTATCCAGTGACAACGGGTTGATTCTGGTGACGGGGGCCACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCGATGGTCGATTTTCTCAACCGCCAGACTGACGGCCATATCCTCACGCTCGAAGATCCGGTGGAG
TTTATGTATCAGAGTGAACGTTGCCTGATTCAGCAGCGCGAGATTGGGCAACACAGCCCGTCCTTTGCCGAGGCGCTACG
CGGCGCATTACGCGAGGATCCGGACGTTATTTTGCTGGGAGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGTGGGGCAGCGCAGGCAATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTGCTTCC
CGATCTCCAGGGCGGGCGCGTCGCGCTGTATGAACTGCTGGTGAACACCGCTGCGGCGGCGAATCTGATTCGTGAAGGGA
AGACGTGGCAACTGCCCGGCATCATTCAAACCGGCCAGCAGGTGGGCATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362