Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RN347_RS02065 Genome accession   NZ_CP134866
Coordinates   449973..451016 (+) Length   347 a.a.
NCBI ID   WP_263585280.1    Uniprot ID   A0AA96EC85
Organism   Halomonas sp. PAMB 3264     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444973..456016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN347_RS02035 (RN347_02035) sfsA 445143..445874 (+) 732 WP_311959770.1 DNA/RNA nuclease SfsA -
  RN347_RS02040 (RN347_02040) metW 445871..446464 (-) 594 WP_263587354.1 methionine biosynthesis protein MetW -
  RN347_RS02045 (RN347_02045) - 446464..447660 (-) 1197 WP_311959773.1 homoserine O-acetyltransferase -
  RN347_RS02050 (RN347_02050) - 447698..448291 (-) 594 WP_311959775.1 YggT family protein -
  RN347_RS02055 (RN347_02055) proC 448346..449170 (-) 825 WP_311959777.1 pyrroline-5-carboxylate reductase -
  RN347_RS02060 (RN347_02060) - 449236..449940 (-) 705 WP_311959779.1 YggS family pyridoxal phosphate-dependent enzyme -
  RN347_RS02065 (RN347_02065) pilT 449973..451016 (+) 1044 WP_263585280.1 type IV pilus twitching motility protein PilT Machinery gene
  RN347_RS02070 (RN347_02070) - 451024..452178 (-) 1155 WP_263585279.1 anhydro-N-acetylmuramic acid kinase -
  RN347_RS02075 (RN347_02075) tyrS 452302..453498 (+) 1197 WP_311959784.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38087.63 Da        Isoelectric Point: 6.0873

>NTDB_id=884182 RN347_RS02065 WP_263585280.1 449973..451016(+) (pilT) [Halomonas sp. PAMB 3264]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIHRLNVPAMSHEQVLSLVHAIMNDRQRQEYDEHLEIDFSYALAEVAR
FRVNAFTQGRGAGAVFRTIPSQVLSMKALGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAALIDYINEYRYEHILTIED
PVEFVHESKSCLVNQREVHRDTHSFANALKSALREDPDVIMVGELRDLETIRLALTAAETGHLVFATLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVVAQTLLKREGGGRVAAHEILIATSAVRNLIREDKVAQIYSAMQTGVNLGMQTLNASL
LKLVKEGAVSFEEAQTRAQGALTPPRD

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=884182 RN347_RS02065 WP_263585280.1 449973..451016(+) (pilT) [Halomonas sp. PAMB 3264]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAACAGAATGCCTCCGACCTGCATCTGAGCGCCGGTTTGCCGCCCAT
GATTCGCGTCGATGGCGATATTCATCGGCTCAACGTCCCCGCCATGAGCCACGAACAGGTGCTGTCGCTGGTTCACGCCA
TCATGAACGACCGTCAACGCCAGGAGTATGACGAGCATCTGGAGATCGACTTCTCCTACGCCTTGGCAGAGGTCGCACGC
TTTCGCGTCAACGCCTTCACCCAGGGGCGTGGCGCCGGGGCGGTGTTTCGCACCATCCCAAGCCAGGTGCTGTCGATGAA
AGCGCTGGGCCTGGGAGAGGTATTCGAGCGCCTGGCAATGCTGCCGCGTGGGCTGGTGCTGGTCACCGGTCCGACCGGGT
CGGGCAAGAGTACGACCCTGGCCGCGCTGATCGACTACATCAACGAATACCGCTATGAGCACATTCTCACCATCGAAGAC
CCGGTGGAATTCGTGCACGAAAGCAAGAGCTGTCTGGTCAATCAACGCGAAGTGCACCGCGATACCCACAGCTTCGCCAA
CGCCTTGAAAAGCGCGCTGCGTGAGGATCCGGACGTGATCATGGTGGGGGAGCTTCGCGATCTCGAAACCATTCGGCTGG
CGCTGACCGCGGCGGAAACCGGGCACCTGGTCTTCGCCACGCTGCACACCACCTCGGCGGCGAAGACCATTGATCGCATC
ATCGACGTTTTCCCCGGCGAGGAGAAAGCCATGGTGCGCTCGATGCTATCGGAGTCGCTGCAGGCGGTCGTCGCCCAGAC
GCTGTTAAAGCGCGAAGGCGGCGGGCGCGTGGCGGCCCACGAAATCCTGATCGCCACCTCGGCGGTGCGCAACCTCATCC
GCGAGGACAAGGTGGCGCAGATCTACTCGGCGATGCAGACCGGCGTCAATCTCGGCATGCAGACCCTCAACGCTTCGCTA
TTAAAGCTGGTCAAGGAGGGCGCGGTCAGCTTCGAGGAGGCTCAGACCCGCGCCCAGGGCGCGCTTACTCCGCCCCGCGA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

74.041

97.695

0.723
  pilT Pseudomonas stutzeri DSM 10701

73.451

97.695

0.718
  pilT Acinetobacter nosocomialis M2

72.566

97.695

0.709
  pilT Acinetobacter baumannii D1279779

72.566

97.695

0.709
  pilT Acinetobacter baumannii strain A118

72.566

97.695

0.709
  pilT Acinetobacter baylyi ADP1

70.501

97.695

0.689
  pilT Legionella pneumophila strain Lp02

70.606

95.101

0.671
  pilT Legionella pneumophila strain ERS1305867

70.606

95.101

0.671
  pilT Neisseria gonorrhoeae MS11

66.374

98.559

0.654
  pilT Neisseria meningitidis 8013

66.374

98.559

0.654
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.667

97.695

0.651
  pilT Vibrio cholerae strain A1552

66.667

97.695

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.622

97.695

0.504
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.542

0.418
  pilU Vibrio cholerae strain A1552

41.52

98.559

0.409
  pilU Acinetobacter baylyi ADP1

40.476

96.83

0.392