Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RN346_RS02030 Genome accession   NZ_CP134865
Coordinates   436418..437461 (+) Length   347 a.a.
NCBI ID   WP_263585280.1    Uniprot ID   A0AA96EC85
Organism   Halomonas sp. PAMB 3232     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 431418..442461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN346_RS02000 (RN346_02000) sfsA 431588..432319 (+) 732 WP_311961969.1 DNA/RNA nuclease SfsA -
  RN346_RS02005 (RN346_02005) metW 432316..432909 (-) 594 WP_263587354.1 methionine biosynthesis protein MetW -
  RN346_RS02010 (RN346_02010) - 432909..434105 (-) 1197 WP_311961970.1 homoserine O-acetyltransferase -
  RN346_RS02015 (RN346_02015) - 434143..434736 (-) 594 WP_263585283.1 YggT family protein -
  RN346_RS02020 (RN346_02020) proC 434791..435615 (-) 825 WP_311961971.1 pyrroline-5-carboxylate reductase -
  RN346_RS02025 (RN346_02025) - 435681..436385 (-) 705 WP_263585281.1 YggS family pyridoxal phosphate-dependent enzyme -
  RN346_RS02030 (RN346_02030) pilT 436418..437461 (+) 1044 WP_263585280.1 type IV pilus twitching motility protein PilT Machinery gene
  RN346_RS02035 (RN346_02035) - 437469..438623 (-) 1155 WP_311961972.1 anhydro-N-acetylmuramic acid kinase -
  RN346_RS02040 (RN346_02040) tyrS 438747..439943 (+) 1197 WP_311959784.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38087.63 Da        Isoelectric Point: 6.0873

>NTDB_id=884164 RN346_RS02030 WP_263585280.1 436418..437461(+) (pilT) [Halomonas sp. PAMB 3232]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIHRLNVPAMSHEQVLSLVHAIMNDRQRQEYDEHLEIDFSYALAEVAR
FRVNAFTQGRGAGAVFRTIPSQVLSMKALGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAALIDYINEYRYEHILTIED
PVEFVHESKSCLVNQREVHRDTHSFANALKSALREDPDVIMVGELRDLETIRLALTAAETGHLVFATLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVVAQTLLKREGGGRVAAHEILIATSAVRNLIREDKVAQIYSAMQTGVNLGMQTLNASL
LKLVKEGAVSFEEAQTRAQGALTPPRD

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=884164 RN346_RS02030 WP_263585280.1 436418..437461(+) (pilT) [Halomonas sp. PAMB 3232]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAACAGAATGCCTCCGACCTGCATCTGAGCGCCGGTTTGCCGCCCAT
GATTCGCGTCGATGGCGATATTCATCGGCTCAACGTCCCCGCCATGAGCCACGAACAGGTGCTGTCGCTGGTTCACGCCA
TCATGAACGACCGTCAACGCCAGGAGTATGACGAGCATCTGGAGATCGACTTCTCCTACGCCTTGGCGGAGGTCGCACGC
TTTCGCGTCAACGCCTTCACCCAGGGGCGTGGCGCCGGGGCGGTGTTTCGCACCATCCCAAGCCAGGTGCTGTCGATGAA
AGCGCTGGGCCTGGGAGAGGTATTCGAGCGCCTGGCAATGCTGCCGCGTGGGCTGGTGCTGGTCACCGGCCCGACCGGGT
CGGGCAAGAGTACGACCCTGGCCGCGCTGATCGACTACATCAACGAATACCGCTATGAGCACATTCTCACCATCGAGGAC
CCGGTGGAATTCGTCCACGAAAGCAAGAGCTGTCTGGTCAATCAACGCGAAGTGCACCGCGACACCCACAGCTTCGCCAA
TGCCTTGAAAAGCGCGCTGCGTGAGGATCCGGACGTGATCATGGTGGGGGAGCTTCGCGATCTCGAAACCATTCGGCTGG
CGCTGACCGCGGCGGAAACCGGGCACCTGGTCTTCGCCACGCTGCACACCACCTCGGCGGCGAAGACCATCGATCGCATC
ATCGACGTTTTCCCCGGCGAGGAGAAAGCCATGGTGCGCTCGATGCTATCGGAGTCGCTGCAGGCGGTCGTCGCCCAGAC
GCTGTTAAAGCGCGAAGGCGGCGGGCGCGTGGCGGCCCACGAAATCCTGATCGCCACCTCGGCGGTGCGCAACCTCATCC
GCGAGGACAAGGTGGCGCAGATCTACTCGGCGATGCAGACCGGCGTCAATCTCGGCATGCAGACCCTCAACGCTTCGCTA
TTAAAGCTGGTCAAGGAGGGCGCGGTCAGCTTCGAGGAGGCTCAGACCCGCGCCCAGGGCGCGCTTACTCCGCCCCGCGA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

74.041

97.695

0.723
  pilT Pseudomonas stutzeri DSM 10701

73.451

97.695

0.718
  pilT Acinetobacter nosocomialis M2

72.566

97.695

0.709
  pilT Acinetobacter baumannii D1279779

72.566

97.695

0.709
  pilT Acinetobacter baumannii strain A118

72.566

97.695

0.709
  pilT Acinetobacter baylyi ADP1

70.501

97.695

0.689
  pilT Legionella pneumophila strain Lp02

70.606

95.101

0.671
  pilT Legionella pneumophila strain ERS1305867

70.606

95.101

0.671
  pilT Neisseria gonorrhoeae MS11

66.374

98.559

0.654
  pilT Neisseria meningitidis 8013

66.374

98.559

0.654
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.667

97.695

0.651
  pilT Vibrio cholerae strain A1552

66.667

97.695

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.622

97.695

0.504
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.542

0.418
  pilU Vibrio cholerae strain A1552

41.52

98.559

0.409
  pilU Acinetobacter baylyi ADP1

40.476

96.83

0.392