Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RKE57_RS02295 Genome accession   NZ_CP134450
Coordinates   494807..495934 (-) Length   375 a.a.
NCBI ID   WP_010480630.1    Uniprot ID   A0A0L8A582
Organism   Stenotrophomonas geniculata strain BR23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 489807..500934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKE57_RS02275 (RKE57_02275) - 490287..491276 (+) 990 WP_010480625.1 fumarylacetoacetate hydrolase family protein -
  RKE57_RS02280 (RKE57_02280) maiA 491291..491974 (+) 684 WP_010480627.1 maleylacetoacetate isomerase -
  RKE57_RS02285 (RKE57_02285) - 492024..493484 (-) 1461 WP_010480628.1 UdgX family uracil-DNA binding protein -
  RKE57_RS02290 (RKE57_02290) - 493465..494748 (-) 1284 WP_010480629.1 putative DNA modification/repair radical SAM protein -
  RKE57_RS02295 (RKE57_02295) pilU 494807..495934 (-) 1128 WP_010480630.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RKE57_RS02300 (RKE57_02300) - 496056..496421 (+) 366 WP_010480631.1 DUF4398 domain-containing protein -
  RKE57_RS02305 (RKE57_02305) - 496418..497302 (+) 885 WP_010480632.1 hypothetical protein -
  RKE57_RS02310 (RKE57_02310) - 497627..497863 (+) 237 WP_005407964.1 YdcH family protein -
  RKE57_RS02315 (RKE57_02315) - 498164..499534 (+) 1371 WP_043398797.1 pyridoxal-phosphate dependent enzyme -
  RKE57_RS02320 (RKE57_02320) - 499658..500833 (+) 1176 WP_311068910.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41410.75 Da        Isoelectric Point: 6.3741

>NTDB_id=880001 RKE57_RS02295 WP_010480630.1 494807..495934(-) (pilU) [Stenotrophomonas geniculata strain BR23]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKAGTIEQEEALRAADSRDGLALKFRLSEGSIGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=880001 RKE57_RS02295 WP_010480630.1 494807..495934(-) (pilU) [Stenotrophomonas geniculata strain BR23]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCCTGACCACCGGAGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTTTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGATACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGGAGACGATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCATTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCAATGGAAGGCTCGCTGGAGGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGTTGGCCAAGGCTGGCACCATCGAGCAGGAGGAGGCCCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCATCGGCGAACACGACCCGTACGCGGATTTCTCGGCCGGCGCGCCGAGCATCACCCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L8A582

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.512

91.733

0.427
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.471

90.667

0.376
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363