Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RJ649_RS05220 Genome accession   NZ_CP134362
Coordinates   1049172..1050152 (+) Length   326 a.a.
NCBI ID   WP_001335651.1    Uniprot ID   A0A454A7C2
Organism   Escherichia coli strain TUM13754     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1044172..1055152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ649_RS05190 (RJ649_05190) yggM 1044777..1045784 (+) 1008 WP_000745230.1 DUF1202 family protein -
  RJ649_RS05195 (RJ649_05195) hemW 1045849..1046985 (-) 1137 WP_000239984.1 radical SAM family heme chaperone HemW -
  RJ649_RS05200 (RJ649_05200) rdgB 1046978..1047571 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  RJ649_RS05205 (RJ649_05205) yggU 1047579..1047869 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  RJ649_RS05210 (RJ649_05210) yggT 1047866..1048432 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  RJ649_RS05215 (RJ649_05215) yggS 1048450..1049154 (-) 705 WP_000997804.1 pyridoxal phosphate homeostasis protein -
  RJ649_RS05220 (RJ649_05220) pilT 1049172..1050152 (+) 981 WP_001335651.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RJ649_RS05225 (RJ649_05225) ruvX 1050326..1050742 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  RJ649_RS05230 (RJ649_05230) yqgE 1050742..1051305 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  RJ649_RS05235 (RJ649_05235) gshB 1051414..1052364 (-) 951 WP_000593259.1 glutathione synthase -
  RJ649_RS05240 (RJ649_05240) rsmE 1052377..1053108 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RJ649_RS05245 (RJ649_05245) endA 1053188..1053895 (-) 708 WP_001298248.1 deoxyribonuclease I -
  RJ649_RS05250 (RJ649_05250) yggI 1053990..1054487 (-) 498 WP_032140833.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36073.30 Da        Isoelectric Point: 5.7980

>NTDB_id=879708 RJ649_RS05220 WP_001335651.1 1049172..1050152(+) (pilT) [Escherichia coli strain TUM13754]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLWEWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=879708 RJ649_RS05220 WP_001335651.1 1049172..1050152(+) (pilT) [Escherichia coli strain TUM13754]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGTGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A454A7C2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365