Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RJ609_RS05805 Genome accession   NZ_CP134269
Coordinates   1438444..1439481 (+) Length   345 a.a.
NCBI ID   WP_036109068.1    Uniprot ID   A0A108U802
Organism   Lysobacter capsici strain XL2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1433444..1444481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ609_RS05780 (RJ609_05780) - 1434193..1435212 (+) 1020 WP_310980574.1 lipase secretion chaperone -
  RJ609_RS05785 (RJ609_05785) - 1435409..1435882 (-) 474 WP_310980575.1 DUF4426 domain-containing protein -
  RJ609_RS05790 (RJ609_05790) proC 1435879..1436715 (-) 837 WP_310980576.1 pyrroline-5-carboxylate reductase -
  RJ609_RS05795 (RJ609_05795) - 1436925..1437449 (+) 525 WP_310980577.1 hypothetical protein -
  RJ609_RS05800 (RJ609_05800) - 1437567..1438268 (-) 702 WP_310980578.1 YggS family pyridoxal phosphate-dependent enzyme -
  RJ609_RS05805 (RJ609_05805) pilT 1438444..1439481 (+) 1038 WP_036109068.1 type IV pilus twitching motility protein PilT Machinery gene
  RJ609_RS05810 (RJ609_05810) pilU 1439587..1440753 (+) 1167 WP_046655750.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38546.39 Da        Isoelectric Point: 6.7270

>NTDB_id=879510 RJ609_RS05805 WP_036109068.1 1438444..1439481(+) (pilT) [Lysobacter capsici strain XL2]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIEQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYAHILSVED
PIEFVHTSQKCLINQREIHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQGYGMMTLDQHL
QDLVKRGMILRPQAREYAKDKRLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=879510 RJ609_RS05805 WP_036109068.1 1438444..1439481(+) (pilT) [Lysobacter capsici strain XL2]
ATGGATATCGCCGAACTACTGGCGTTCTCGGTTAAGAACAAAGCATCCGACTTGCATCTGTCCGCGGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTGCGCCGGATCAACATCCCGGCCCTGGACCACAAACAGGTCCATGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTTCGAAGAATTCCTCGAGGTCGACTTCTCCTTCGAAATCCCCGGCCTGGCGCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGTACCATTCCCTCCGAAGTGCTGACCCTGGA
AGACCTCGGCTGCCCGCCGATCTTCCGTCAGCTCATCGAACAGCCGCAGGGCCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCGGCGATGATCGACCACATCAACAAGAACGAATACGCGCACATCCTGTCGGTGGAAGAC
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTAATCAACCAGCGCGAAATCCACCGCGATACGCACGGGTTCAACGA
GGCCCTGCGCTCGGCGTTGCGCGAAGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACTTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCGTCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAAAAACCGATGGTGCGCTCGATGCTGTCGGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTCAAGAAGGTCGGCGGCGGCCGTACCGCGGCCTGGGAAATCATGGTCGGCATCCCGGCGATCCGAAACCTCATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCCAGGGCTACGGCATGATGACGCTCGATCAGCATCTG
CAGGACCTGGTCAAGCGCGGCATGATCCTGCGCCCGCAGGCGCGCGAGTACGCGAAGGACAAGCGCCTGTTCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A108U802

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.71

0.774
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

73.256

99.71

0.73
  pilT Legionella pneumophila strain ERS1305867

73.256

99.71

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.605

95.362

0.664
  pilT Vibrio cholerae strain A1552

69.605

95.362

0.664
  pilT Neisseria meningitidis 8013

65.789

99.13

0.652
  pilT Neisseria gonorrhoeae MS11

65.497

99.13

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38