Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RHJ80_RS04915 Genome accession   NZ_CP134181
Coordinates   1042901..1043998 (-) Length   365 a.a.
NCBI ID   WP_149817851.1    Uniprot ID   -
Organism   Thermosynechococcus sp. QS41     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1037901..1048998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHJ80_RS04885 (RHJ80_04885) mreD 1038320..1038847 (+) 528 WP_310801581.1 rod shape-determining protein MreD -
  RHJ80_RS13175 - 1038893..1039063 (-) 171 Protein_954 hypothetical protein -
  RHJ80_RS04890 (RHJ80_04890) - 1039311..1039993 (-) 683 Protein_955 IS630 transposase-related protein -
  RHJ80_RS04895 (RHJ80_04895) - 1040543..1040896 (+) 354 WP_206201314.1 hypothetical protein -
  RHJ80_RS04900 (RHJ80_04900) - 1040893..1041165 (-) 273 WP_149817855.1 DUF3143 domain-containing protein -
  RHJ80_RS04905 (RHJ80_04905) - 1041162..1041635 (-) 474 WP_310801582.1 J domain-containing protein -
  RHJ80_RS04910 (RHJ80_04910) - 1041644..1042864 (-) 1221 WP_310801583.1 type II secretion system F family protein -
  RHJ80_RS04915 (RHJ80_04915) pilT 1042901..1043998 (-) 1098 WP_149817851.1 type IV pilus twitching motility protein PilT Machinery gene
  RHJ80_RS04920 (RHJ80_04920) pilB/pilB1 1044066..1046090 (-) 2025 WP_310801584.1 GspE/PulE family protein Machinery gene
  RHJ80_RS04925 (RHJ80_04925) - 1046352..1046564 (+) 213 WP_206201319.1 hypothetical protein -
  RHJ80_RS04930 (RHJ80_04930) - 1046565..1046762 (+) 198 WP_206201320.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  RHJ80_RS04935 (RHJ80_04935) - 1046759..1048285 (+) 1527 WP_310744171.1 right-handed parallel beta-helix repeat-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40366.43 Da        Isoelectric Point: 6.7579

>NTDB_id=879178 RHJ80_RS04915 WP_149817851.1 1042901..1043998(-) (pilT) [Thermosynechococcus sp. QS41]
MELMIEDLMEQVVANGGSDLHISAGLPPYIRISGKLTPTDYEPLTPEQCQRLIFSMLNNTQRKHLEQNWELDCSYGVRGL
ARFRVNVYKDRGTYAACLRALSSKIPTFEQLGLPNIVREMSERPRGLILVTGPTGSGKTTTLAAMIDLINKTRAEHILTI
EDPIEFVYEPIKSLIHQRQVGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQQQIRVQLSNSLVAVFSQTLVPKKNPKPGEFGRIMAQEIMVVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYRAGIITYEAAMAKSSRQDELQRLIGTGTPAAAAR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=879178 RHJ80_RS04915 WP_149817851.1 1042901..1043998(-) (pilT) [Thermosynechococcus sp. QS41]
ATGGAGCTAATGATTGAAGACCTAATGGAGCAAGTGGTGGCCAATGGTGGCTCCGATTTGCATATCTCCGCTGGCTTACC
CCCCTACATTCGCATTAGCGGCAAATTAACCCCGACGGACTACGAACCCCTCACCCCAGAGCAATGCCAGCGCCTGATCT
TCAGCATGCTCAACAATACCCAACGCAAGCACCTAGAGCAAAACTGGGAGTTGGACTGCTCCTATGGTGTACGTGGCCTC
GCCCGGTTCCGCGTCAATGTATATAAAGACCGGGGGACCTATGCTGCTTGCTTGCGAGCACTCAGTTCCAAAATTCCCAC
GTTTGAGCAGTTGGGATTGCCCAACATTGTCCGCGAAATGAGTGAGCGGCCACGGGGCTTGATTCTGGTGACTGGGCCGA
CAGGATCAGGGAAAACCACAACCTTGGCGGCGATGATTGACTTGATCAACAAAACCCGCGCTGAGCATATCCTGACGATT
GAAGACCCCATTGAGTTTGTCTATGAACCCATCAAGAGCCTGATCCACCAACGGCAGGTGGGGGAAGATACCAAGAGCTT
TGCCAATGCTCTGCGGGCAGCGCTGCGGGAAGACCCCGATATCATCCTTGTGGGTGAGATGCGTGACTTGGAAACAATTC
AGCTTGCCATCTCAGCAGCGGAAACAGGGCACTTGGTCTTTGGTACCTTGCACACCAGTTCAGCCGCCCAAACCGTTGAC
CGTATGGTGGATGTGTTCCCGCCAGAGCAGCAACAGCAAATTCGCGTCCAGTTGTCGAACTCGTTGGTGGCGGTCTTTAG
CCAAACGCTGGTTCCTAAGAAAAATCCCAAGCCCGGTGAATTTGGGCGGATTATGGCGCAAGAAATTATGGTGGTGACCC
CTGCTATCTCCAACCTGATTCGCGAAGGCAAAACCTCGCAGATTTATTCGGCAATTCAAACGGGCGGCAAGCTGGGGATG
CAGACCCTTGAAAAAGTCTTGGCCGATTATTATCGTGCGGGCATCATCACCTATGAAGCAGCAATGGCCAAGTCCTCGCG
TCAGGACGAGTTGCAACGTCTGATTGGTACGGGTACACCTGCGGCAGCAGCACGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.425

93.425

0.518
  pilT Legionella pneumophila strain ERS1305867

54.203

94.521

0.512
  pilT Legionella pneumophila strain Lp02

54.203

94.521

0.512
  pilT Pseudomonas stutzeri DSM 10701

53.936

93.973

0.507
  pilT Acinetobacter baylyi ADP1

54.572

92.877

0.507
  pilT Acinetobacter baumannii strain A118

53.412

92.329

0.493
  pilT Acinetobacter nosocomialis M2

53.412

92.329

0.493
  pilT Acinetobacter baumannii D1279779

53.412

92.329

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.286

95.89

0.482
  pilT Neisseria meningitidis 8013

49.284

95.616

0.471
  pilT Neisseria gonorrhoeae MS11

49.284

95.616

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

93.151

0.466
  pilT Vibrio cholerae strain A1552

50

93.151

0.466
  pilU Pseudomonas stutzeri DSM 10701

39.155

97.26

0.381
  pilU Acinetobacter baylyi ADP1

38.109

95.616

0.364