Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RHJ80_RS04920 Genome accession   NZ_CP134181
Coordinates   1044066..1046090 (-) Length   674 a.a.
NCBI ID   WP_310801584.1    Uniprot ID   -
Organism   Thermosynechococcus sp. QS41     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1039066..1051090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHJ80_RS04890 (RHJ80_04890) - 1039311..1039993 (-) 683 Protein_955 IS630 transposase-related protein -
  RHJ80_RS04895 (RHJ80_04895) - 1040543..1040896 (+) 354 WP_206201314.1 hypothetical protein -
  RHJ80_RS04900 (RHJ80_04900) - 1040893..1041165 (-) 273 WP_149817855.1 DUF3143 domain-containing protein -
  RHJ80_RS04905 (RHJ80_04905) - 1041162..1041635 (-) 474 WP_310801582.1 J domain-containing protein -
  RHJ80_RS04910 (RHJ80_04910) - 1041644..1042864 (-) 1221 WP_310801583.1 type II secretion system F family protein -
  RHJ80_RS04915 (RHJ80_04915) pilT 1042901..1043998 (-) 1098 WP_149817851.1 type IV pilus twitching motility protein PilT Machinery gene
  RHJ80_RS04920 (RHJ80_04920) pilB/pilB1 1044066..1046090 (-) 2025 WP_310801584.1 GspE/PulE family protein Machinery gene
  RHJ80_RS04925 (RHJ80_04925) - 1046352..1046564 (+) 213 WP_206201319.1 hypothetical protein -
  RHJ80_RS04930 (RHJ80_04930) - 1046565..1046762 (+) 198 WP_206201320.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  RHJ80_RS04935 (RHJ80_04935) - 1046759..1048285 (+) 1527 WP_310744171.1 right-handed parallel beta-helix repeat-containing protein -
  RHJ80_RS04940 (RHJ80_04940) - 1048282..1049244 (-) 963 WP_310744172.1 hypothetical protein -
  RHJ80_RS04945 (RHJ80_04945) - 1049335..1050291 (+) 957 WP_310801585.1 glycosyltransferase -
  RHJ80_RS04950 (RHJ80_04950) leuD 1050284..1050874 (-) 591 WP_302679005.1 3-isopropylmalate dehydratase small subunit -

Sequence

Protein


Download         Length: 674 a.a.        Molecular weight: 74377.69 Da        Isoelectric Point: 5.4297

>NTDB_id=879179 RHJ80_RS04920 WP_310801584.1 1044066..1046090(-) (pilB/pilB1) [Thermosynechococcus sp. QS41]
MVNTSSSSSRKALTVRGRAASPTERAIVASGYASIDQVREAMNTARKTGKSLVTVLQEITGTTMPPDVLRQYHKQQLFEL
KVIYGVDCLDPELNRFPTEQIEELINTIVPIDTCRTYQVIPIAKHLDADPPYLLVAMVDPDNLQAIDNLTRLLRAHNLTL
KRMVITLEDYQRLIDPILNKQVAETTASKSAPAAIGDINIEEDIEAMGGLEELEGEQEVDLAEALKGAEDAPIIALVNKI
LAKALTEGVSDIHIEPQEEYLRIRFRKDGVLHQAFDPLPKKIVPAVVSRFKILADLDIAERRAPQDGRIRKMFQGRRVDF
RVNTLPSRWGEKVVLRILDNSATQLGLDKLITDPESLAIVREMTKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVNIS
TAEDPIEYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPSAIAR
LSEMGIEPFMVSASLIGVVAQRLMRRVCSECRIPYTPTREELARFGLSASKDVNLTLYKANKLTPEQIQAAKATGQPICS
KCGGLGYKGRVGVYEIMRVTERLQSLITEGAPTEQIKEAAVEEGMKTLLAYSLNLVKEGVTTLEEVERVTFTDTGLESEL
KAKRKSSLTCRTCGAEAQPEWLECPYCMTPRFVD

Nucleotide


Download         Length: 2025 bp        

>NTDB_id=879179 RHJ80_RS04920 WP_310801584.1 1044066..1046090(-) (pilB/pilB1) [Thermosynechococcus sp. QS41]
ATGGTCAACACATCGTCATCTTCTTCTCGGAAAGCACTTACCGTGCGCGGCAGAGCCGCTAGCCCCACAGAACGAGCCAT
TGTTGCCTCCGGTTACGCCAGTATCGATCAGGTGCGGGAAGCCATGAACACCGCCCGCAAAACGGGCAAGTCCCTCGTAA
CCGTGTTGCAGGAGATTACGGGCACCACCATGCCCCCCGATGTGTTACGCCAGTACCACAAGCAACAGCTTTTTGAACTC
AAAGTGATCTACGGGGTTGATTGCCTCGATCCCGAACTCAATCGTTTCCCCACCGAGCAAATCGAGGAACTGATCAACAC
GATTGTACCGATTGACACCTGCCGCACCTACCAAGTCATCCCCATTGCCAAGCACCTCGACGCTGATCCCCCCTATCTGC
TCGTGGCGATGGTGGATCCAGATAACCTCCAAGCCATTGACAACCTCACCCGCTTACTCCGCGCCCACAACCTCACCCTC
AAGCGAATGGTGATCACCCTAGAGGATTACCAACGCCTCATTGACCCGATTCTCAATAAACAGGTAGCTGAAACCACCGC
TAGCAAAAGCGCCCCGGCTGCGATCGGTGACATCAACATCGAAGAAGACATCGAAGCCATGGGCGGCCTCGAAGAGCTGG
AAGGGGAGCAGGAGGTTGACCTTGCTGAGGCGCTGAAAGGTGCAGAAGATGCTCCGATTATTGCCCTTGTCAACAAAATT
TTGGCCAAAGCCCTCACCGAAGGCGTTTCCGACATTCACATCGAACCTCAAGAGGAATACCTGCGGATTCGTTTCCGCAA
AGATGGGGTGCTTCACCAAGCCTTTGATCCACTGCCGAAGAAAATCGTGCCAGCCGTCGTTTCCCGCTTCAAAATCTTGG
CTGACCTTGATATTGCCGAGCGGCGTGCCCCCCAAGATGGTCGTATCCGCAAAATGTTCCAAGGGCGACGGGTGGACTTC
CGAGTGAATACCCTCCCCAGCCGCTGGGGCGAAAAAGTCGTACTGCGGATTCTCGATAACTCCGCCACCCAACTCGGTCT
CGACAAGCTGATTACTGACCCTGAGAGCCTTGCTATTGTGCGGGAGATGACCAAGCGCCCCTTTGGCCTCATCCTCGTTA
CAGGTCCCACTGGTTCAGGGAAAACCACCACGCTTTACTCTGCGCTGGCTGAATGTAATAGTCCCGGTGTCAACATCAGT
ACAGCGGAAGACCCGATTGAATACACGCTCCCCGGCCTAACGCAGGTACAGGTTATTCGCGAAAAAGGCATGGACTTTGC
CTCAATTTTGCGAGCCTTTCTCCGCCAAGACCCCGATGTAATTCTGGTGGGCGAAACCCGTGACAAAGAAACCGCAAAAA
CCGCCATTGAAGCGGCTTTGACGGGGCACTTGGTCTTGACAACCCTGCACACTAACGATGCCCCCAGTGCAATTGCCCGC
CTCTCGGAAATGGGCATTGAACCCTTTATGGTTTCCGCCTCCCTCATTGGGGTTGTTGCCCAACGGCTGATGCGGCGGGT
ATGCAGTGAGTGTCGGATTCCCTATACACCCACCCGTGAGGAACTGGCGCGCTTTGGCTTGTCTGCCTCCAAGGATGTCA
ACCTCACCCTCTACAAAGCCAATAAACTGACACCCGAGCAGATTCAAGCGGCTAAGGCAACTGGCCAACCCATTTGCAGT
AAATGTGGTGGTTTGGGCTATAAAGGACGGGTCGGCGTCTACGAAATTATGCGGGTGACGGAGCGCCTGCAAAGCCTGAT
TACTGAGGGTGCCCCCACCGAGCAAATCAAGGAGGCGGCCGTCGAAGAGGGCATGAAAACCCTGTTGGCCTATAGCCTGA
ACCTTGTCAAAGAAGGGGTCACCACCCTCGAGGAAGTCGAGCGCGTCACCTTTACGGACACGGGTCTCGAATCAGAACTC
AAGGCCAAACGTAAGAGTTCTTTAACCTGCCGTACCTGCGGTGCCGAGGCGCAGCCCGAATGGTTAGAATGCCCCTATTG
CATGACACCCCGCTTTGTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(239-506)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(74-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.244

97.329

0.635
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.462

94.51

0.364