Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RHH53_RS04960 Genome accession   NZ_CP134169
Coordinates   1036798..1037895 (-) Length   365 a.a.
NCBI ID   WP_206201317.1    Uniprot ID   -
Organism   Thermosynechococcus sp. PKX82     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1031798..1042895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHH53_RS04930 (RHH53_04930) mreD 1032221..1032748 (+) 528 WP_302676618.1 rod shape-determining protein MreD -
  RHH53_RS13220 - 1032794..1032964 (-) 171 Protein_957 hypothetical protein -
  RHH53_RS04935 (RHH53_04935) - 1033212..1033890 (-) 679 Protein_958 IS630 transposase-related protein -
  RHH53_RS04940 (RHH53_04940) - 1034440..1034793 (+) 354 WP_206201314.1 hypothetical protein -
  RHH53_RS04945 (RHH53_04945) - 1034790..1035062 (-) 273 WP_149817855.1 DUF3143 domain-containing protein -
  RHH53_RS04950 (RHH53_04950) - 1035059..1035532 (-) 474 WP_206201315.1 J domain-containing protein -
  RHH53_RS04955 (RHH53_04955) - 1035541..1036761 (-) 1221 WP_206201316.1 type II secretion system F family protein -
  RHH53_RS04960 (RHH53_04960) pilT 1036798..1037895 (-) 1098 WP_206201317.1 type IV pilus twitching motility protein PilT Machinery gene
  RHH53_RS04965 (RHH53_04965) pilB/pilB1 1037963..1039987 (-) 2025 WP_302679001.1 GspE/PulE family protein Machinery gene
  RHH53_RS04970 (RHH53_04970) - 1040249..1040461 (+) 213 WP_206201319.1 hypothetical protein -
  RHH53_RS04975 (RHH53_04975) - 1040462..1040659 (+) 198 WP_206201320.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  RHH53_RS04980 (RHH53_04980) - 1040656..1042182 (+) 1527 WP_310744171.1 right-handed parallel beta-helix repeat-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40352.41 Da        Isoelectric Point: 6.7579

>NTDB_id=878986 RHH53_RS04960 WP_206201317.1 1036798..1037895(-) (pilT) [Thermosynechococcus sp. PKX82]
MELMIEDLMEQVVANGGSDLHISAGLPPYIRISGKLTPTDYEPLTPEQCQRLIFSMLNNTQRKHLEQNWELDCSYGVRGL
ARFRVNVYKDRGTYAACLRALSSKIPTFEQLGLPNVVREMSERPRGLILVTGPTGSGKTTTLAAMIDLINKTRAEHILTI
EDPIEFVYEPIKSLIHQRQVGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQQQIRVQLSNSLVAVFSQTLVPKKNPKPGEFGRIMAQEIMVVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYRAGIITYEAAMAKSSRQDELQRLIGTGTPAAAAR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=878986 RHH53_RS04960 WP_206201317.1 1036798..1037895(-) (pilT) [Thermosynechococcus sp. PKX82]
ATGGAGCTAATGATTGAAGACCTAATGGAGCAAGTGGTGGCCAATGGTGGCTCCGATTTGCATATCTCCGCTGGCTTACC
CCCCTACATTCGCATTAGCGGCAAATTAACCCCGACCGACTACGAACCCCTCACCCCAGAGCAATGCCAGCGCCTGATTT
TCAGCATGCTCAACAATACCCAACGCAAGCACCTAGAGCAAAACTGGGAGTTGGACTGCTCCTATGGTGTACGTGGCCTC
GCCCGGTTCCGCGTCAATGTGTATAAAGACCGGGGAACCTATGCTGCTTGCTTGCGAGCACTCAGTTCCAAAATTCCCAC
GTTTGAGCAGTTGGGATTGCCCAATGTTGTCCGCGAAATGAGTGAGCGGCCACGGGGCTTGATTCTGGTGACTGGGCCGA
CAGGATCAGGGAAAACCACAACCTTGGCGGCGATGATTGACTTGATCAACAAAACCCGCGCTGAGCATATCCTGACGATT
GAAGACCCCATTGAGTTTGTCTATGAACCCATCAAGAGCCTGATCCACCAACGGCAGGTGGGGGAAGATACCAAGAGCTT
TGCCAATGCTCTGCGGGCAGCGCTGCGGGAAGACCCCGATATTATCCTTGTGGGTGAGATGCGGGACTTGGAAACAATTC
AGCTTGCCATCTCAGCGGCAGAAACCGGTCACTTGGTCTTTGGTACCTTGCACACCAGTTCAGCTGCCCAAACCGTTGAC
CGTATGGTGGATGTGTTCCCGCCAGAGCAGCAGCAGCAAATTCGCGTCCAGTTGTCCAACTCGTTAGTGGCGGTCTTTAG
CCAAACGCTGGTTCCTAAGAAAAATCCCAAGCCCGGTGAATTTGGGCGGATTATGGCGCAAGAAATTATGGTGGTGACCC
CTGCTATCTCCAACCTGATTCGCGAAGGCAAAACCTCGCAGATTTATTCGGCAATTCAAACGGGCGGCAAGCTGGGGATG
CAGACCCTTGAAAAAGTCTTGGCCGATTATTATCGTGCGGGCATCATCACCTATGAAGCAGCAATGGCCAAGTCCTCGCG
TCAGGACGAGTTGCAACGTCTGATTGGTACGGGTACACCTGCGGCAGCAGCACGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.718

93.425

0.521
  pilT Legionella pneumophila strain ERS1305867

53.913

94.521

0.51
  pilT Legionella pneumophila strain Lp02

53.913

94.521

0.51
  pilT Pseudomonas stutzeri DSM 10701

54.227

93.973

0.51
  pilT Acinetobacter baylyi ADP1

54.277

92.877

0.504
  pilT Acinetobacter baumannii strain A118

53.116

92.329

0.49
  pilT Acinetobacter nosocomialis M2

53.116

92.329

0.49
  pilT Acinetobacter baumannii D1279779

53.116

92.329

0.49
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.286

95.89

0.482
  pilT Neisseria gonorrhoeae MS11

48.997

95.616

0.468
  pilT Neisseria meningitidis 8013

48.997

95.616

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.706

93.151

0.463
  pilT Vibrio cholerae strain A1552

49.706

93.151

0.463
  pilU Pseudomonas stutzeri DSM 10701

39.437

97.26

0.384
  pilU Acinetobacter baylyi ADP1

37.822

95.616

0.362