Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RHH53_RS04965 Genome accession   NZ_CP134169
Coordinates   1037963..1039987 (-) Length   674 a.a.
NCBI ID   WP_302679001.1    Uniprot ID   -
Organism   Thermosynechococcus sp. PKX82     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1032963..1044987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHH53_RS04935 (RHH53_04935) - 1033212..1033890 (-) 679 Protein_958 IS630 transposase-related protein -
  RHH53_RS04940 (RHH53_04940) - 1034440..1034793 (+) 354 WP_206201314.1 hypothetical protein -
  RHH53_RS04945 (RHH53_04945) - 1034790..1035062 (-) 273 WP_149817855.1 DUF3143 domain-containing protein -
  RHH53_RS04950 (RHH53_04950) - 1035059..1035532 (-) 474 WP_206201315.1 J domain-containing protein -
  RHH53_RS04955 (RHH53_04955) - 1035541..1036761 (-) 1221 WP_206201316.1 type II secretion system F family protein -
  RHH53_RS04960 (RHH53_04960) pilT 1036798..1037895 (-) 1098 WP_206201317.1 type IV pilus twitching motility protein PilT Machinery gene
  RHH53_RS04965 (RHH53_04965) pilB/pilB1 1037963..1039987 (-) 2025 WP_302679001.1 GspE/PulE family protein Machinery gene
  RHH53_RS04970 (RHH53_04970) - 1040249..1040461 (+) 213 WP_206201319.1 hypothetical protein -
  RHH53_RS04975 (RHH53_04975) - 1040462..1040659 (+) 198 WP_206201320.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  RHH53_RS04980 (RHH53_04980) - 1040656..1042182 (+) 1527 WP_310744171.1 right-handed parallel beta-helix repeat-containing protein -
  RHH53_RS04985 (RHH53_04985) - 1042179..1043141 (-) 963 WP_310744172.1 hypothetical protein -
  RHH53_RS04990 (RHH53_04990) - 1043232..1044188 (+) 957 WP_310744173.1 glycosyltransferase -
  RHH53_RS04995 (RHH53_04995) leuD 1044181..1044771 (-) 591 WP_286626447.1 3-isopropylmalate dehydratase small subunit -

Sequence

Protein


Download         Length: 674 a.a.        Molecular weight: 74516.89 Da        Isoelectric Point: 5.4345

>NTDB_id=878987 RHH53_RS04965 WP_302679001.1 1037963..1039987(-) (pilB/pilB1) [Thermosynechococcus sp. PKX82]
MVNTSSSSSRKALTVRGRAASPTERAIVASGYASIDQVREAMNTARKTGKSLVTVLQEITGTTMPPDVLRQYHKQQLFEL
KVIYGVDCLDPELNRFPTEQIEELINTIVPIDTCRTYQVIPIAKHLDADPPYLLVAMVDPDNLQAIDNLTRLLRAHNLTL
KRMVITLEDYQRLIDPILNKQVAETTASKNAPAEIGDINIEEDIEAMGSLEELEGEQEVDLAEALKGAEDAPIIALVNKI
LAKALTEGVSDIHIEPQEEYLRIRFRKDGVLHQAFDPLPKKIVPAVVSRFKILADLDIAERRAPQDGRIRKMFQGRRVDF
RVNTLPSRWGEKVVLRILDNSATQLGLDKLITDPESLAIVREMTKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVNIS
TAEDPIEYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPSAIAR
LSEMGIEPFMVSASLIGVVAQRLMRRVCSECRIPYTPTREELARFGLSASKDVNLTLYKANKLTPEQIQAAKATGQPICS
KCGGLGYKGRVGVYEIMRITERLQRLITEGAPTEQIKEAAVEEGMKTLLAYSLGLVKEGLTTLEEVERVTFTDTGLEAEL
KAKRKSSLTCRTCGAEAQPEWLECPYCMTPRFVD

Nucleotide


Download         Length: 2025 bp        

>NTDB_id=878987 RHH53_RS04965 WP_302679001.1 1037963..1039987(-) (pilB/pilB1) [Thermosynechococcus sp. PKX82]
ATGGTCAACACATCGTCATCTTCTTCTCGGAAAGCACTTACCGTGCGCGGCAGAGCCGCTAGCCCCACAGAACGAGCCAT
TGTTGCCTCCGGTTACGCCAGTATCGATCAGGTGCGGGAAGCCATGAACACCGCCCGCAAAACGGGCAAGTCCCTCGTAA
CCGTGTTGCAGGAGATTACGGGCACCACCATGCCCCCCGATGTGTTACGCCAGTACCACAAGCAACAGCTTTTTGAACTC
AAAGTGATCTACGGGGTTGATTGCCTCGATCCCGAACTCAATCGTTTCCCCACCGAGCAAATCGAGGAACTGATCAACAC
GATTGTACCGATTGACACCTGCCGCACCTACCAAGTCATCCCCATTGCCAAGCACCTCGACGCTGATCCCCCCTATCTGC
TCGTGGCGATGGTGGATCCAGATAACCTCCAAGCCATTGACAACCTCACCCGCTTACTCCGCGCCCACAACCTCACCCTC
AAGCGAATGGTGATCACCCTAGAGGATTACCAACGCCTCATTGACCCGATTCTCAATAAACAGGTAGCTGAAACCACCGC
TAGCAAAAATGCCCCGGCTGAGATCGGTGACATCAACATCGAAGAAGACATCGAAGCCATGGGCAGCCTCGAAGAGCTGG
AAGGGGAGCAGGAGGTTGACCTTGCTGAGGCGCTGAAAGGTGCAGAAGATGCGCCGATTATTGCCCTTGTCAACAAAATT
TTGGCCAAAGCCCTCACCGAAGGCGTTTCCGACATTCACATCGAACCTCAAGAGGAATACCTGCGGATTCGTTTCCGCAA
AGATGGGGTGCTTCACCAAGCCTTTGATCCACTGCCGAAGAAAATCGTGCCAGCCGTCGTTTCCCGCTTCAAAATCTTGG
CTGACCTTGATATTGCTGAGCGGCGTGCCCCCCAAGATGGTCGTATCCGCAAAATGTTCCAAGGGCGACGGGTGGACTTC
CGAGTGAATACCCTCCCCAGCCGCTGGGGCGAAAAAGTCGTACTGCGGATTCTCGATAACTCCGCCACCCAACTCGGTCT
CGACAAGCTGATTACTGACCCTGAGAGCCTTGCTATTGTGCGGGAGATGACCAAGCGCCCCTTTGGCCTCATCCTCGTTA
CAGGTCCCACTGGTTCAGGGAAAACCACCACGCTTTACTCTGCGCTGGCGGAGTGTAATAGTCCCGGTGTCAACATCAGT
ACAGCGGAAGACCCGATTGAATACACGCTCCCCGGCCTAACGCAGGTACAGGTTATTCGCGAAAAAGGCATGGACTTTGC
CTCAATTTTGCGAGCCTTTCTCCGCCAAGACCCCGATGTAATTCTGGTGGGCGAAACCCGTGACAAAGAAACCGCAAAAA
CCGCCATTGAAGCGGCTTTGACGGGGCACTTGGTCTTGACAACCCTGCACACTAACGATGCCCCCAGTGCAATTGCCCGC
CTCTCGGAAATGGGCATTGAACCCTTTATGGTTTCCGCCTCCCTCATTGGGGTTGTTGCCCAACGGCTGATGCGGCGGGT
ATGCAGTGAGTGTCGGATTCCCTATACACCCACCCGTGAGGAACTGGCGCGCTTTGGCTTGTCTGCCTCCAAGGATGTCA
ACCTCACCCTCTACAAAGCCAATAAACTGACACCCGAGCAAATTCAAGCGGCTAAGGCAACTGGCCAACCCATTTGCAGT
AAATGTGGTGGTTTGGGCTACAAAGGACGGGTCGGCGTCTACGAAATTATGCGGATCACCGAGCGACTGCAACGGCTGAT
TACCGAAGGTGCCCCCACCGAGCAAATCAAGGAGGCGGCCGTCGAAGAGGGCATGAAAACCCTGCTTGCCTATAGCTTGG
GGTTAGTCAAGGAAGGACTCACCACCCTCGAAGAAGTGGAGCGCGTCACCTTTACGGACACTGGACTAGAGGCAGAACTC
AAGGCCAAACGTAAGAGTTCTTTAACCTGCCGTACCTGCGGTGCCGAGGCGCAGCCCGAATGGTTAGAATGCCCCTATTG
CATGACACCCCGCTTTGTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(239-506)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(74-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.985

97.033

0.631
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.776

94.51

0.366